Results 31 to 40 of about 2,624,902 (305)
Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose. [PDF]
PLoS ONE, 2014 We previously reported that multiply-primed rolling circle amplification (MRPCA) using modified random RNA primers can amplify tiny amounts of circular DNA without producing any byproducts.
Hirokazu Takahashi +10 more
doaj +1 more source
DNA polymerases and cancer [PDF]
Nature Reviews Cancer, 2011 There are 15 different DNA polymerases encoded in mammalian genomes, which are specialized for replication, repair or the tolerance of DNA damage. New evidence is emerging for lesion-specific and tissue-specific functions of DNA polymerases. Many point mutations that occur in cancer cells arise from the error-generating activities of DNA polymerases ...
Sabine S, Lange +2 more
openaire +2 more sources
Engineering processive DNA polymerases with maximum benefit and minimum cost
Frontiers in Microbiology, 2014 DNA polymerases need to be engineered to achieve optimal performance for biotechnological applications, which often require high fidelity replication when using modified nucleotides and when replicating difficult DNA sequences.
Linda J. Reha-Krantz +2 more
doaj +1 more source
A nuclear family A DNA polymerase from Entamoeba histolytica bypasses thymine glycol. [PDF]
PLoS Neglected Tropical Diseases, 2010 BACKGROUND: Eukaryotic family A DNA polymerases are involved in mitochondrial DNA replication or translesion DNA synthesis. Here, we present evidence that the sole family A DNA polymerase from the parasite protozoan E.
Guillermo Pastor-Palacios +2 more
doaj +1 more source
DNA Polymerase Profiling [PDF]
, 2008 We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template strand. A resulting distance alteration is reported by fluorescence resonance energy transfer between two dyes ...
openaire +3 more sources
Topoisomerase IIα promotes activation of RNA polymerase I transcription by facilitating pre-initiation complex formation [PDF]
, 2013 Type II DNA topoisomerases catalyse DNA double-strand cleavage, passage and re-ligation to effect topological changes. There is considerable interest in elucidating topoisomerase II roles, particularly as these proteins are targets for anti-cancer drugs.
Porter, Andrew C. G. +22 more
core +1 more source
Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors
Frontiers in Bioengineering and BiotechnologyWe present a live culture PCR (LC-PCR) workflow that enables direct screening of randomly mutagenized Thermus aquaticus (Taq) and Klentaq1 DNA polymerase libraries without enzyme purification.
Milko B. Kermekchiev +3 more
doaj +1 more source
Brazilian Journal of Medical and Biological Research, 1998 For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that
R.S. Diaz, E.C. Sabino
doaj +1 more source
Bst polymerase — a humble relative of Taq polymerase
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair.
Igor Oscorbin, Maxim Filipenko
doaj +1 more source
Contributions of ubiquitin- and PCNA-binding domains to the activity of Polymerase η in Saccharomyces cerevisiae [PDF]
, 2007 Bypassing of DNA lesions by damage-tolerant DNA polymerases depends on the interaction of these enzymes with the monoubiquitylated form of the replicative clamp protein, PCNA.
Bielen, Aleksandra B. +3 more
core +1 more source