Results 31 to 40 of about 1,030,716 (269)

REV1 restrains DNA polymerase zeta to ensure frame fidelity during translesion synthesis of UV photoproducts in vivo

, 2008
Exposure to ultraviolet light induces a number of forms of damage in DNA, of which (6-4) photoproducts present the most formidable challenge to DNA replication.
Marcus, AP, Iwai, S, Szüts, D, Himoto, M, Sale, JE   +4 more
core   +1 more source

Bacteriophage T7 DNA polymerase — Sequenase

Frontiers in Microbiology, 2014
An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: 1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; 2) high processivity; 3) high ...
Bin eZhu
doaj   +1 more source

Bst polymerase — a humble relative of Taq polymerase

Computational and Structural Biotechnology Journal, 2023
DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair.
Igor Oscorbin, Maxim Filipenko
doaj   +1 more source

A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification

Iranian Journal of Microbiology, 2019
Background and Objectives: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3´ to 5´ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs).
Prabu Siva Sankar, Marimuthu Citartan, Aminah Ahmed Siti, Boris V. Skryabin, Timofey S. Rozhdestvensky, Goot Heah Khor, Thean Hock Tang   +6 more
doaj   +1 more source

Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches

Bioengineering, 2023
DNA-dependent DNA polymerases have been intensively studied for more than 60 years and underlie numerous biotechnological and diagnostic applications.
Aleksandra A. Kuznetsova, Nikita A. Kuznetsov   +1 more
doaj   +1 more source

DNA polymerase α (swi7) and the flap endonuclease fen1 (rad2) act together in the s-phase alkylation damage response in S. pombe [PDF]

, 2012
Polymerase α is an essential enzyme mainly mediating Okazaki fragment synthesis during lagging strand replication. A specific point mutation in Schizosaccharomyces pombe polymerase α named swi7-1, abolishes imprinting required for mating-type switching ...
Koulintchenko Milana, Koulintchenko, Milana, Milana Koulintchenko, Sonya Vengrova, Vengrova S., Dalgaard Jacob Z., Trevor Eydmann, Prakash Arumugam, Eydmann Trevor, Koulintchenko M., Arumugam P., Vengrova, Sonya, Arumugam Prakash, Jacob Z. Dalgaard, Arumugam, Prakash, Eydmann T., Eydmann, Trevor, Dalgaard, Jacob Z., Jacob Z Dalgaard, Dalgaard J.Z., Vengrova Sonya   +20 more
core   +1 more source

Collision with duplex DNA renders Escherichia coli DNA polymerase III holoenzyme susceptible to DNA polymerase IV-mediated polymerase switching on the sliding clamp

Scientific Reports, 2017
Organisms possess multiple DNA polymerases (Pols) and use each for a different purpose. One of the five Pols in Escherichia coli, DNA polymerase IV (Pol IV), encoded by the dinB gene, is known to participate in lesion bypass at certain DNA adducts.
Thanh Thi Le, Asako Furukohri, Masahiro Tatsumi-Akiyama, Hisaji Maki   +3 more
doaj   +1 more source

Fidelity of DNA polymerases in the detection of intraindividual variation of mitochondrial DNA

Mitochondrial DNA. Part B. Resources, 2020
Here we investigated the consequences of PCR amplification errors in the identification of intraindividual mtDNA variation. The bumblebee Bombus morio was chosen as model for the COI gene amplification tests with two DNA polymerases (Taq and Q5 ...
Paulo Cseri Ricardo, Elaine Françoso, Maria Cristina Arias   +2 more
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway

, 2015
DNA polymerase theta (Polθ) has been identified as a crucial alternative non-homologous end-joining factor in mammalian cells. Polθ is upregulated in a range of cancer cell types defective in homologous recombination, and knockdown has been shown to ...
Aitkenhead, H, Gileadi, Opher, Cooper, Christopher D O, Newman, JA, Cooper, CD, Gileadi, O, Cooper, Christopher D.O., Newman, Joseph A., Aitkenhead, Hazel   +8 more
core   +1 more source