Results 31 to 40 of about 1,506,691 (357)

Molecular dissection of the domain architecture and catalytic activities of human PrimPol [PDF]

, 2014
PrimPol is a primase–polymerase involved in nuclear and mitochondrial DNA replication in eukaryotic cells. Although PrimPol is predicted to possess an archaeo-eukaryotic primase and a UL52-like zinc finger domain, the role of these domains has not been ...
Aidan J. Doherty, Aravind, Benjamin A. Keen, Bianchi, Biertümpfel, Biswas, Bocquier, Boudsocq, Brautigam, Chen, Chen, Connolly, Corn, Della, Duncan, Engelke, Fogg, Frick, Garcia-Gomez, Graziewicz, Griep, Hays, Iyer, Johnson, Julie Bianchi, Kim, Lao-Sirieix, Lao-Sirieix, Laura J. Bailey, Lehmann, Lipps, Liu, Maga, Masutani, Mendelman, Ohashi, Pelletier, Pinz, Pitcher, Rudd, Shannon, Stanislaw K. Jozwiakowski, Tabor, van Loon, Wan, Washington   +45 more
core   +2 more sources

DNA polymerases in Biotechnology [PDF]

Frontiers in Microbiology, 2014
DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology.
Gardner, Andrew F., Kelman, Zvi
openaire   +5 more sources

Proficient replication of the yeast genome by a viral DNA polymerase [PDF]

, 2016
DNA replication in eukaryotic cells requires minimally three B-family DNA polymerases: Pol α, Pol δ, and Pol ϵ. Pol δ replicates and matures Okazaki fragments on the lagging strand of the replication fork.
Burgers, Peter M, Stith, Carrie M, Stodola, Joseph L   +2 more
core   +2 more sources

DNA Polymerase-Catalyzed DNA Network Growth [PDF]

Journal of the American Chemical Society, 2008
The distinct base pairing property of DNA is an advantageous phenomenon that has been exploited in the usage of DNA as scaffold for directed self-organization to form nanometer-sized objects in a desirable fashion. Herein we report the construction of three-dimensional DNA-based networks that can be generated and amplified by the DNA polymerase chain ...
Keller, Sascha, Marx, Andreas, Wang, Jie, Chandra, Madhaviah, Berger, Rüdiger   +4 more
openaire   +4 more sources

Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

Genes and Environment, 2022
Urea (Ua) is produced in DNA as the result of oxidative damage to thymine and guanine. It was previously reported that Klenow fragment (Kf) exo− incorporated dATP opposite Ua, and that DNA polymerase β was blocked by Ua.
Taishu Kawada, Katsuhito Kino, Kyousuke Tokorodani, Ryuto Anabuki, Masayuki Morikawa, Takanobu Kobayashi, Kazuaki Ohara, Takayuki Ohshima, Hiroshi Miyazawa   +8 more
doaj   +1 more source

Polymerase Mu Is a DNA-Directed DNA/RNA Polymerase [PDF]

Molecular and Cellular Biology, 2003
DNA polymerases are defined as such because they use deoxynucleotides instead of ribonucleotides with high specificity. We show here that polymerase mu (pol μ), implicated in the nonhomologous end-joining pathway for repair of DNA double-strand breaks, incorporates both ribonucleotides and deoxynucleotides in a template-directed manner.
Stephanie A, Nick McElhinny, Dale A, Ramsden   +1 more
openaire   +2 more sources

Mismatch repair in recombination of bacteriophage T4

Biomolecular Concepts, 2012
The review focuses on the mechanism of mismatch repair in bacteriophage T4. It was first observed in T4 as an extra recombination mechanism, which contributed to the general recombination only when particular rII mutations were used as genetic markers ...
Shcherbakov Victor P.
doaj   +1 more source

A novel role of DNA polymerase λ in translesion synthesis in conjunction with DNA polymerase ζ

Life Science Alliance, 2021
As an integral scaffolding component of DNA polymerase (Pol) zeta, Pol lambda adapts Pol zeta–dependent translesion synthesis to operate in a predominantly error-free manner in human cells.
Jung-Hoon Yoon, Debashree Basu, Karthi Sellamuthu, Robert E Johnson, Satya Prakash, Louise Prakash   +5 more
doaj   +1 more source

Exploration of factors driving incorporation of unnatural dNTPS into DNA by Klenow fragment (DNA polymerase I) and DNA polymerase {alpha} [PDF]

, 2006
In order to further understand how DNA polymerases discriminate against incorrect dNTPs, we synthesized two sets of dNTP analogues and tested them as substrates for DNA polymerase a (pol alpha) and Klenow fragment (exo-) of DNA polymerase I (Escherichia ...
Kincaid, Kristi, Beckman, Jeff, Zivkovic, Aleksandra, Halcomb, Randall L., Engels, Joachim W. (Prof. Dr.), Kuchta, Robert D.   +5 more
core   +1 more source

Promutagenicity of 8-Chloroguanine, A Major Inflammation-Induced Halogenated DNA Lesion

Molecules, 2019
Chronic inflammation is closely associated with cancer development. One possible mechanism for inflammation-induced carcinogenesis is DNA damage caused by reactive halogen species, such as hypochlorous acid, which is released by myeloperoxidase to kill ...
Yi Kou, Myong-Chul Koag, Seongmin Lee
doaj   +1 more source