Results 61 to 70 of about 1,030,716 (269)

Subtype‐specific enhancer RNAs define transcriptional regulators and prognosis in breast cancers

Molecular Oncology, EarlyView.
This study employed machine learning methodologies to perform the subtype‐specific classification of RNA‐seq data sets, which are mapped on enhancers from TCGA‐derived breast cancer patients. Their integration with gene expression (referred to as ProxCReAM eRNAs) and chromatin accessibility profiles has the potential to identify lineage‐specific and ...
Aamena Y. Patel, Peyman Zarrineh, Nathnael T. Tuffa, Jigar H. Sheth, Sumitra Mohan, Mudassar Iqbal, Sankari Nagarajan   +6 more
wiley   +1 more source

Insights into genotoxic effects of electromagnetic fields [PDF]

, 2008
The increasing use of appliances, which generate electromagnetic fields (EMFs), has provoked public concern about their safety. Scientific research into possible health effects however produced conflicting results.
Focke, Frauke
core   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Interactions of DNA with a new Platinum(IV) Azide Dipyridine complex activated by UVA and visible light : relationship to toxicity in tumor cells [PDF]

, 2012
The Pt IV diazido complex trans,trans,trans-[Pt(N 3) 2(OH) 2(pyridine) 2] (1) is unreactive in the dark but is cytotoxic when photoactivated by UVA and visible light.
Sadler, P. J., Jitka Pracharova, Pracharova, Jitka, Kasparkova, Jana, Jana Stepankova, Farrer, Nicola J., Brabec, V. (Viktor), Lenka Zerzankova, Nicola J. Farrer, Peter J. Sadler, Jana Kasparkova, Zerzankova, Lenka, Novakova, Olga, Stepankova, Jana, Viktor Brabec, Olga Novakova   +15 more
core   +1 more source

Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering

Frontiers in Microbiology, 2014
DNA polymerases are widely used for DNA manipulation in vitro, including DNA cloning, sequencing, DNA labeling, mutagenesis, and other experiments. Thermostable DNA polymerases are especially useful and became quite valuable after the development of PCR ...
Takeshi eYamagami, Sonoko eIshino, Yutaka eKawarabayasi, Yutaka eKawarabayasi, Yoshizumi eIshino   +4 more
doaj   +1 more source

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

Molecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos, Gilar Gorji‐bahri, Lejla Gradjan, Julia Zajac, Kacper Gil, Yvonne Thokozile Nduku, Anna M. Blom   +6 more
wiley   +1 more source

Analyses of the archaeal transcription cycle reveal a mosaic of eukaryotic RNA polymerase II and III-like features

, 2009
Novel mechanistic properties of the archaeal transcription system that reveal similarities especially to the eukaryotic RNA polymerase II and III systems were presented in thesis.
Spitalny, Patrizia
core   +1 more source

A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification

BioTechniques, 2014
The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used.
Konstantin B. Ignatov, Ekaterina V. Barsova, Arkady F. Fradkov, Konstantin A. Blagodatskikh, Tatiana V. Kramarova, Vladimir M. Kramarov   +5 more
doaj   +1 more source

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Chimerization of antibodies by isolation of rearranged genomic variable regions by the polymerase chain reaction [PDF]

, 1991
We describe a new method for amplification, by polymerase chain reaction (PCR), of rearranged segments encoding the variable part of light and heavy chains of an antibody (Ab) from the chromosomal DNA of hybridoma cells for the chimerization ofAbs.
Schwirzke, Marina, Kaluzab, Brigitte, Weissenhorn, Winfried, Weiss, Elisabeth H., Weidle, Ulrich H.   +4 more
core   +1 more source