Theory of Nucleosome Corkscrew Sliding in the Presence of Synthetic DNA Ligands
Histone octamers show a heat-induced mobility along DNA. Recent theoretical studies have established two mechanisms that are qualitatively and quantitatively compatible with in vitro experiments on nucleosome sliding: Octamer repositiong through one ...
Kulic, Igor M. +2 more
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The Design and Fabrication of Platform Device for Dna Amplification [PDF]
Thermalcycler were extensively used machine for amplify DNA sample. One of the major problems in the working time was that it spent most of time for cooling and heating.
Chien, Ch. -Heng, Yu, Hui-Min
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White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase
White spot syndrome virus (WSSV) is the causative agent of white spot syndrome, one of the most devastating diseases in shrimp aquaculture. The genome of WSSV includes a gene that encodes a putative family B DNA polymerase (ORF514), which is 16 ...
Rogerio R. Sotelo-Mundo +7 more
doaj +1 more source
DNA extraction and polymerase chain reaction
DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
Nalini Gupta
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Titular: Consejo Superior de Investigaciones Científicas (CSIC)
Blanco Dávila, Luis +2 more
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COMPARISON OF SIX COMMERCIALLY-AVAILABLE DNA POLYMERASES FOR DIRECT PCR
SUMMARY The use of a “direct PCR” DNA polymerase enables PCR amplification without any prior DNA purification from blood samples due to the enzyme's resistance to inhibitors present in blood components. Such DNA polymerases are now commercially available.
Masashi Miura +3 more
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T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids. [PDF]
Body fluid DNA sequencing is a powerful noninvasive approach for the diagnosis of genetic defects, infectious agents and diseases. The success relies on the quantity and quality of the DNA samples.
Chen, Chien-Jen +8 more
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An interaction between replication protein A and SV40 T antigen appears essential for primosome assembly during SV40 DNA replication [PDF]
Replication protein A from human cells (hRPA) is a multisubunit single-stranded DNA-binding protein (ssb) and is essential for SV40 DNA replication in vitro.
Melendy, T., Stillman, B.
core
Mutations that Separate the Functions of the Proofreading Subunit of the Escherichia coli Replicase
The dnaQ gene of Escherichia coli encodes the Ɛ subunit of DNA polymerase III, which provides the 3\u27 - 5\u27 exonuclease proofreading activity of the replicative polymerase.
Kreuzer, Kenneth N., Whatley, Zakiya
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Evidence for DNA-mediated nuclear compartmentalization distinct from phase separation. [PDF]
RNA Polymerase II (Pol II) and transcription factors form concentrated hubs in cells via multivalent protein-protein interactions, often mediated by proteins with intrinsically disordered regions.
Darzacq, Xavier +9 more
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