Results 91 to 100 of about 1,454,497 (332)

Effectiveness of annealing blocking primers versus restriction enzymes for characterization of generalist diets: unexpected prey revealed in the gut contents of two coral reef fish species. [PDF]

PLoS ONE, 2013
Characterization of predator-prey interactions is challenging as researchers have to rely on indirect methods that can be costly, biased and too imprecise to elucidate the complexity of food webs.
Matthieu Leray, Natalia Agudelo, Suzanne C Mills, Christopher P Meyer   +3 more
doaj   +1 more source

Single cis‐elements in brassinosteroid‐induced upregulated genes are insufficient to recruit both redox states of the BIL1/BZR1 DNA‐binding domain

FEBS Letters, EarlyView.
Phytohormone brassinosteroid‐induced gene regulation by the transcription factor BIL1/BZR1 involves redox‐dependent DNA‐binding alternation and interaction with the transcription factor PIF4. The reduced BIL1/BZR1 dimer binds preferred cis‐elements, while oxidation alters its oligomerization state and disrupts DNA‐binding ability.
Shohei Nosaki, Masae Ohtsuka, Takeshi Nakano, Masaru Tanokura, Takuya Miyakawa   +4 more
wiley   +1 more source

Rapid identification of European (Anguilla anguilla) and North American eel (Anguilla rostrata) by polymerase chain reaction. [PDF]

, 2006
A rapid and cost effective DNA test is described to identify European eel (Anguilla anguilla) and North American eel (Anguilla rostrata). By means of polymerase chain reaction (PCR) technique parts of the mitochondrial cytochrome b gene are amplified ...
Trautner, Jochen
core  

Human Cytomegalovirus: detection of congenital and perinatal infection in Argentina [PDF]

, 2004
BACKGROUND: Human cytomegalovirus (CMV) is one of the most commonly found agents of congenital infections. Primary maternal infection is associated with risk of symptomatic congenital diseases, and high morbidity is frequently associated with very low ...
Alicia Alonso, Angélica Lidia Distéfano, B Zweygberg Wirgart, D Shibata, DJ Morris, E Gonczol, Fabián Martin, Fabián Pardon, GJ Demmler, J Darlington, JC Mendez, JR Murph, KB Fowler, M Barbi, M Barbi, M Brytting, M Shibata, MG Revello, MG Revello, NM Jiwa, PD Griffiths, PV Coyle, RJ Whitley, S Chou, SA Plotkin, SB Boppana   +25 more
core   +3 more sources

C9orf72 ALS‐causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules

FEBS Letters, EarlyView.
Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient‐derived neurons and C. elegans models, we find that the nucleoporin Nup107 is dysregulated in C9orf72‐associated ALS. Conversely, reducing Nup107 levels mitigates disease‐related changes.
Saygın Bilican, Yara Nabawi, William Hongyu Zhang, Dunja Petrovic, Markus Wehrmann, Sara Muñoz‐García, Seda Koyuncu, David Vilchez   +7 more
wiley   +1 more source

In silico estimation of annealing specificity of query searches in DNA databases [PDF]

, 2005
We consider DNA implementations of databases for digital signals with retrieval and mining capabilities. Digital signals are encoded in DNA sequences and retrieved through annealing between query DNA primers and data carrying DNA target sequences.
Hatzimanikati, Vassily, Katsaggelos, Aggelos K., Tsaftaris, Sotirios A.   +2 more
core  

Desenvolvimento de um método molecular para detecção e identificação de Xanthomonas campestris pv. viticola [PDF]

, 2007
Com o objetivo de desenvolver um método molecular para detecção e identificação de Xanthomonas campestris pv. viticola (Xcv), agente causal do cancro bacteriano da videira, oligonucleotídeos (primers) foram desenhados com base na seqüência parcial do ...
Ferreira, Marisa Alvares da Silva Velloso, Lopes, Daniela Biaggioni, Marques, Eder, Trindade, Loiselene Carvalho da   +3 more
core   +1 more source

Cancer cell death induced by the NAD antimetabolite Vacor discloses the antitumor potential of SARM1

FEBS Letters, EarlyView.
Vacor, a compound converted into the toxic metabolite Vacor adenine dinucleotide (VAD) by the nicotinamide salvage pathway enzymes NAMPT and NMNAT2, exhibits antitumor activity by inducing rapid and complete NAD depletion. We report that Vacor toxicity is limited to cell lines expressing high levels of SARM1, a NAD glycohydrolase.
Giuseppe Ranieri, Andrea Lapucci, Giuseppe Orsomando, Nadia Raffaelli, Alberto Chiarugi, Daniela Buonvicino   +5 more
wiley   +1 more source

Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices [PDF]

, 2015
Background: In order to provide a system fully integrated with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMOs in food/feed matrices, two bidirectional DNA walking ...
De Loose, Marc, Deforce, Dieter, Fraiture, Marie-Alice, Herman, Philippe, Lefèvre, Loic, Roosens, Nancy H, Taverniers, Isabel   +6 more
core   +6 more sources

RAD50 missense variants differentially affect the DNA damage response and mitotic progression

FEBS Letters, EarlyView.
RAD50 incorporates into the MRN complex and initiates the DNA damage response. Furthermore, RAD50 promotes mitotic progression. RAD50 missense variants capable of forming an MRN complex supported the DNA damage response and mitotic features to different extents in complementation experiments, indicating these functions are separable and might impact ...
Hanna Redeker, Swantje Kebel, Lea Völkening, Anna Vatselia, Louisa Weinhold, Girmay Asgedom, Axel Schambach, Detlev Schindler, Thilo Dörk, Kristine Bousset   +9 more
wiley   +1 more source