A hybrid G-quadruplex structure formed between RNA and DNA explains the extraordinary stability of the mitochondrial R-loop [PDF]
In human mitochondria the transcription machinery generates the RNA primers needed for initiation of DNA replication. A critical feature of the leading-strand origin of mitochondrial DNA replication is a CG-rich element denoted conserved sequence block ...
Falkenberg, Maria+5 more
core +1 more source
AFLP: a new technique for DNA fingerprinting.
A novel DNA fingerprinting technique called AFLP is described. The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic DNA.
P. Vos+10 more
semanticscholar +1 more source
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro+2 more
wiley +1 more source
Development of RAPD, DAMD and ISSR markers for authentication of medicinal plant Cassia auriculata and its adulterant Cassia surattensis [PDF]
Cassia auriculata is an important traditional medicinal plant commonly used in many Ayurvedic formulations, meant for diabetes, rheumatism, conjunctivitis, infertility and etc.
M, P. P. (Panapitiya), N, W. (Welikala)
core
Template-dependent multiple displacement amplification for profiling human circulating RNA [PDF]
Multiple displacement amplification (MDA) is widely used in whole-genome/transcriptome amplification. However, template-independent amplification (TIA) in MDA is a commonly observed phenomenon, particularly when using high concentrations of random ...
Crosby, Seth D+6 more
core +2 more sources
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt+4 more
wiley +1 more source
Simultaneous Qualitative And Quantitative Identification Of Multiple Targets By Polymerase Chain Reaction On Microarray [PDF]
An approach for multiplex qualitative and quantitative microarray-based PCR analysis has been proposed. The characteristics of PCR executed on a gel-based oligonucleotide microarray with immobilized forward primers and a single common reverse primer in solution were investigated for several DNA targets.
arxiv
Polymerase/nicking enzyme powered dual-template multi-cycled G-triplex machine for HIV-1 determination [PDF]
We proposed a dual-template multi-cycled DNA nanomachine driven by polymerase nicking enzyme with high efficiency. The reaction system simply consists of two templates (T1, T2) and two enzymes (KF polymerase, Nb.BbvCI). The two templates are similar in structure (X-X-Y, Y-Y-C): primer recognition region, primer analogue generation region, output region
arxiv
Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules [PDF]
We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure ...
arxiv +1 more source
Evolutionary interplay between viruses and R‐loops
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi+4 more
wiley +1 more source