Results 111 to 120 of about 1,454,497 (332)

Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability In Citrus (P148) [PDF]

open access: yes, 2011
Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences (BES) of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and ...
Navarro, Luis   +5 more
core  

Family-specific degenerate primer design: a tool to design consensus degenerated oligonucleotides [PDF]

open access: yes, 2013
Designing degenerate PCR primers for templates of unknown nucleotide sequence may be a very difficult task. In this paper, we present a new method to design degenerate primers, implemented in family-specific degenerate primer design (FAS-DPD) computer ...
Borio, Cristina Silvia   +5 more
core   +3 more sources

Reciprocal control of viral infection and phosphoinositide dynamics

open access: yesFEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Rapid, solid-phase based automated analysis of chromatin structure and transcription factor occupancy in living eukaryotic cells [PDF]

open access: yes, 2005
Transcription factors, chromatin components and chromatin modification activities are involved in many diseases including cancer. However, the means by which alterations in these factors influence the epigenotype of specific cell types is poorly ...
Bonifer, C.   +3 more
core   +2 more sources

Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples

open access: yesMitochondrial DNA. Part B. Resources, 2017
We describe six novel ungulate-specific conserved primers for sequencing the complete mitochondrial cytochrome c oxidase I (COI) gene of selected threatened species from degraded samples for effective conservation planning.
Ajit Kumar   +5 more
doaj   +1 more source

A large‐scale retrospective study in metastatic breast cancer patients using circulating tumour DNA and machine learning to predict treatment outcome and progression‐free survival

open access: yesMolecular Oncology, EarlyView.
There is an unmet need in metastatic breast cancer patients to monitor therapy response in real time. In this study, we show how a noninvasive and affordable strategy based on sequencing of plasma samples with longitudinal tracking of tumour fraction paired with a statistical model provides valuable information on treatment response in advance of the ...
Emma J. Beddowes   +20 more
wiley   +1 more source

PCR-based identification of toxinogenic Fusarium species

open access: yesCzech Journal of Food Sciences, 2009
Aim of this study was to develop sensitive PCR assay for mycotoxin producing Fusarium species. Strains Fusarium oxysporum 4199 and Fusarium culmorum 4044 were used as representatives of this group.
Iva Lacmanová   +3 more
doaj   +1 more source

Multidimensional OMICs reveal ARID1A orchestrated control of DNA damage, splicing, and cell cycle in normal‐like and malignant urothelial cells

open access: yesMolecular Oncology, EarlyView.
Loss of the frequently mutated chromatin remodeler ARID1A, a subunit of the SWI/SNF cBAF complex, results in less open chromatin, alternative splicing, and the failure to stop cells from progressing through the cell cycle after DNA damage in bladder (cancer) cells. Created in BioRender. Epigenetic regulators, such as the SWI/SNF complex, with important
Rebecca M. Schlösser   +11 more
wiley   +1 more source

Analysis of DNA Fragment Obtained from Groupers (Epinephelus fuscoguttatus) Challenged by Vibrio alginolyticus

open access: yesJurnal Ilmu Dasar, 2010
The objective of this study was to analyse the size of DNA fragment from both resistant and susceptible groupers (Epinephelus fuscoguttatus) after infection by Vibrio alginolyticus.
St Hidayah Triana
doaj  

The design and testing of mini-barcode markers in marine lobsters.

open access: yesPLoS ONE, 2019
Full-length mitochondrial cytochrome c oxidase I (COI) sequence information from lobster phyllosoma larvae can be difficult to obtain when DNA is degraded or fragmented. Primers that amplify smaller fragments are also more useful in metabarcoding studies.
Ashrenee Govender   +3 more
doaj   +1 more source

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