Results 281 to 290 of about 1,066,757 (310)

“Primer” in DNA Polymerase Reactions

1963
Publisher Summary This chapter reviews two degrees of primer activation. The first concerns the secondary structure of the DNA molecule, and for the calf thymus and phage-induced E. coli polymerase this type of activation, typically produced by heat, acid, or alkaline denaturation, results in rate increases of one to two orders of magnitude.
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DNA Extraction and Primer Selection

2013
Talk regarding pitfalls in DNA extraction and 16S amplicon primer choice when performing community analysis of complex microbial communities. The talk was a part of Workshop 2 "Principles, Potential, and Limitations of Novel Molecular Methods in Water Engineering; from Amplicon Sequencing to -omics Methods" given at the MEWE 2013 conference in Ann ...
Karst, Søren Michael; id_orcid 0000-0002-0233-160X, Nielsen, Per Halkjær, Albertsen, Mads; id_orcid 0000-0002-6151-190X, Ziegler, Anja Sloth   +3 more
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DNA Synthesis by Primer Exchange Reaction Cascades

ChemBioChem, 2018
Swap and extend: The autonomous synthesis of single-stranded DNA molecules of arbitrary size and sequence composition can easily be achieved by primer exchange reaction (PER) cascades, in which the sequential polymerase-mediated extension of DNA primers is guided by catalytic hairpins.
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HPV DNA Forward and Reverse Primer Identification

2023
[ES] Los investigadores amplifican manualmente determinadas secuencias sintéticas de ADN del virus del papiloma humano (VPH) para facilitar la detección y discriminación de múltiples tipos de VPH. El objetivo de esta investigación era crear un programa informático que, utilizando parámetros seleccionados por el usuario, pudiera encontrar ...
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Designing PCR Primer for DNA Methylation Mapping

2007
DNA methylation is an epigenetic mechanism of gene regulation, and aberrant methylation has been associated with various types of diseases, especially cancers. Detection of DNA methylation has thus become an important approach for studying gene regulation and has potential diagnostic application.
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DNA sequencing and primer extension mapping

1997
The two DNA sequencing techniques currently in use are the chemical degradation method developed by Maxam and Gilbert (1979) and the enzymatic method developed by Sanger et al. (1977). Due to the less laborious protocol and the easy availability of oligonucleotides, the ‘Sanger’ method is now the most widely used one, being applicable not only for the ...
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A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322

Gene, 1981
R Bhatt, R B Wallace, M J Johnson
exaly  

Studies on the minimal lengths required for DNA primers to be extended by the Tetrahymena telomerase: implications for primer positioning by the enzyme

Nucleic Acids Research, 2002
Haim Manor
exaly  

Application of DNA barcoding in fish identification of supermarkets in Henan province, China: More and longer COI gene sequences were obtained by designing new primers

Food Research International, 2020
Chuanjiang Zhou, Yongtao Tang, Wenwen Ma   +2 more
exaly  

New primers for DNA barcoding of digeneans and cestodes (Platyhelminthes)

Molecular Ecology Resources, 2015
Niels Van Steenkiste, David Marcogliese, Magalie Castelin   +2 more
exaly