Results 261 to 270 of about 1,480,527 (305)

Multiple‐primer DNA sequencing method

ELECTROPHORESIS, 2003
AbstractA multiple‐primer DNA sequencing approach suitable for genotyping, detection and identification of microorganisms and viruses has been developed. In this new method two or more sequencing primers, combined in a pool, are added to a DNA sample of interest. The oligonucleotide that hybridizes to the DNA sample will function as a primer during the
Baback, Gharizadeh, Mehran, Ghaderi, Declan, Donnelly, Bahram, Amini, Keng-Ling, Wallin, Pål, Nyrén   +5 more
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Specific-primer-directed DNA sequencing

Analytical Biochemistry, 1986
A simple and rapid strategy for DNA sequence analysis based on the Sanger chain-termination method is described. This procedure utilizes full-sized inserts of 1 to 4 kb of DNA cloned into M13 bacteriophage vectors. After the sequence of the first 600-650 bp of the insert DNA has been determined with the commercially available universal vector primer, a
E C, Strauss, J A, Kobori, G, Siu, L E, Hood   +3 more
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DNA sequencing with arbitrary primer pairs

Molecular Ecology, 1994
Keywords: molecular population genetics; nucleotide diversity; nucleotide polymorphism; PCR; RAPDs ...
A, Burt, D A, Carter, T J, White, J W, Taylor   +3 more
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A primer to scaffolded DNA origami

Nature Methods, 2011
Molecular self-assembly with scaffolded DNA origami enables building custom-shaped nanometer-scale objects with molecular weights in the megadalton regime. Here we provide a practical guide for design and assembly of scaffolded DNA origami objects. We also introduce a computational tool for predicting the structure of DNA origami objects and provide ...
Carlos Ernesto, Castro, Fabian, Kilchherr, Do-Nyun, Kim, Enrique Lin, Shiao, Tobias, Wauer, Philipp, Wortmann, Mark, Bathe, Hendrik, Dietz   +7 more
openaire   +2 more sources

Are “universal” DNA primers really universal?

Journal of Applied Genetics, 2014
"Universal" DNA primers LCO 1490 and HCO 2198 were originally designed from three coding and six anticoding strands by comparing highly conserved regions of mitochondrial cytochrome c oxidase subunit I (COI) genes across 15 taxa. These primers have been successful in amplifying a 710-bp fragment of highly conserved regions of the COI gene for more than
Sharma, P., Kobayashi, T.
openaire   +3 more sources

Nuclear DNA Primers

1998
Since its introduction in 1985, the polymerase chain reaction (PCR) (1) has profoundly affected the way in which molecular studies are conducted. Olsen et al. (2) first proposed that short single copy DNA sequences could be amplified by PCR from cloned DNA using specific primers.
Joanne Russell, Gordon C. Machray, Robbie Waugh   +2 more
openaire   +1 more source

DNA Editing: A Primer

Blood, 2015
Abstract RNA-guided Cas9 nucleases based on the prokaryotic CRISPR-Cas systems provide an unprecedented ease in being able to edit the genomes of diverse organisms. As a single unifying factor capable of co-localizing RNA, DNA, and protein, I believe tools and techniques based on Cas9 will grant exquisite control over cellular ...
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Universal COI primers for DNA barcoding amphibians

Molecular Ecology Resources, 2011
AbstractDNA barcoding is a proven tool for the rapid and unambiguous identification of species, which is essential for many activities including the vouchering tissue samples in the genome 10K initiative, genealogical reconstructions, forensics and biodiversity surveys, among many other applications.
Jing, Che, Hong-Man, Chen, Jun-Xiao, Yang, Jie-Qiong, Jin, Ke, Jiang, Zhi-Yong, Yuan, Robert W, Murphy, Ya-Ping, Zhang   +7 more
openaire   +2 more sources

Primers for Animal Mitochondrial DNA: The Importance of Species-Specific Primers

1998
In this chapter we outline the procedure for designing oligonucleotide primers for the direct-sequencing of in vitro amplified animal mitochondrial DNA (mtDNA). Although this chapter deals with animal mtDNA in particular, the approach is of course applicable to any gene, nuclear or mitochondrial. There are four main ‘steps’:identification of a suitable
Palsboll, Per, ARCTANDER, P
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DNA amplification using PCR with abutting primers

Molecular Biology, 2015
DNA analysis of ñîmplex biological objects (wastewater, soil, archaeological and forensic samples, etc.) is currently of great interest. DNA of these objects is characterized by low suitability for research due to the violation of its integrity and chemical structure; thus, the detection of specific nucleic acid fragments can be achieved by PCR with ...
R R, Garafutdinov, A A, Galimova, A R, Sakhabutdinova, V A, Vakhitov, A V, Chemeris   +4 more
openaire   +2 more sources