Results 261 to 270 of about 1,066,757 (310)

DNA amplification using PCR with abutting primers

Molecular Biology, 2015
DNA analysis of ñîmplex biological objects (wastewater, soil, archaeological and forensic samples, etc.) is currently of great interest. DNA of these objects is characterized by low suitability for research due to the violation of its integrity and chemical structure; thus, the detection of specific nucleic acid fragments can be achieved by PCR with ...
Ravil Garafutdinov, Chemeris A V, Garafutdinov R R   +2 more
exaly   +3 more sources

A primer to scaffolded DNA origami

Nature Methods, 2011
Molecular self-assembly with scaffolded DNA origami enables building custom-shaped nanometer-scale objects with molecular weights in the megadalton regime. Here we provide a practical guide for design and assembly of scaffolded DNA origami objects. We also introduce a computational tool for predicting the structure of DNA origami objects and provide ...
Carlos Ernesto, Castro, Fabian, Kilchherr, Do-Nyun, Kim, Enrique Lin, Shiao, Tobias, Wauer, Philipp, Wortmann, Mark, Bathe, Hendrik, Dietz   +7 more
openaire   +2 more sources

Multiple‐primer DNA sequencing method

ELECTROPHORESIS, 2003
AbstractA multiple‐primer DNA sequencing approach suitable for genotyping, detection and identification of microorganisms and viruses has been developed. In this new method two or more sequencing primers, combined in a pool, are added to a DNA sample of interest. The oligonucleotide that hybridizes to the DNA sample will function as a primer during the
Baback, Gharizadeh, Mehran, Ghaderi, Declan, Donnelly, Bahram, Amini, Keng-Ling, Wallin, Pål, Nyrén   +5 more
openaire   +2 more sources

DNA sequencing by synthesis with degenerate primers

Journal of Genetics and Genomics, 2008
The degenerate primer-based sequencing was developed by a synthesis method (DP-SBS) for high-throughput DNA sequencing, in which a set of degenerate primers are hybridized on the arrayed DNA templates and extended by DNA polymerase on microarrays.
Chao, Tang, Xiaolong, Shi, Xiujie, Li, Zuhong, Lu   +3 more
openaire   +2 more sources

Fluorescent labeling of a DNA sequencing primer

DNA Sequence, 1993
Several oligonucleotides containing one to four fluorescein labels in various positions were synthesized and the fluorescence intensity and thermal stability of the duplex forms with their complementary sequences were measured. Oligomers that contain two fluorescein molecules, one attached at the 5' terminus and the other at an internal phosphate, were
J, Mineno, Y, Ishino, T, Ohminami, I, Kato   +3 more
openaire   +2 more sources

An Environmental DNA Primer for Microbial and Restoration Ecology

Microbial Ecology, 2023
Environmental DNA (eDNA) sequencing-DNA collected from the environment from living cells or shed DNA-was first developed for working with microbes and has greatly benefitted microbial ecologists for decades since. These tools have only become increasingly powerful with the advent of metabarcoding and metagenomics.
Michael Tessler, Seth W. Cunningham, Melissa R. Ingala, Sally D. Warring, Mercer R. Brugler   +4 more
openaire   +2 more sources

DNA sequencing with arbitrary primer pairs

Molecular Ecology, 1994
Keywords: molecular population genetics; nucleotide diversity; nucleotide polymorphism; PCR; RAPDs ...
A, Burt, D A, Carter, T J, White, J W, Taylor   +3 more
openaire   +2 more sources

Specific-primer-directed DNA sequencing

Analytical Biochemistry, 1986
A simple and rapid strategy for DNA sequence analysis based on the Sanger chain-termination method is described. This procedure utilizes full-sized inserts of 1 to 4 kb of DNA cloned into M13 bacteriophage vectors. After the sequence of the first 600-650 bp of the insert DNA has been determined with the commercially available universal vector primer, a
E C, Strauss, J A, Kobori, G, Siu, L E, Hood   +3 more
openaire   +2 more sources