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Are “universal” DNA primers really universal?
Journal of Applied Genetics, 2014"Universal" DNA primers LCO 1490 and HCO 2198 were originally designed from three coding and six anticoding strands by comparing highly conserved regions of mitochondrial cytochrome c oxidase subunit I (COI) genes across 15 taxa. These primers have been successful in amplifying a 710-bp fragment of highly conserved regions of the COI gene for more than
Sharma, P., Kobayashi, T.
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1998
Since its introduction in 1985, the polymerase chain reaction (PCR) (1) has profoundly affected the way in which molecular studies are conducted. Olsen et al. (2) first proposed that short single copy DNA sequences could be amplified by PCR from cloned DNA using specific primers.
Joanne Russell +2 more
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Since its introduction in 1985, the polymerase chain reaction (PCR) (1) has profoundly affected the way in which molecular studies are conducted. Olsen et al. (2) first proposed that short single copy DNA sequences could be amplified by PCR from cloned DNA using specific primers.
Joanne Russell +2 more
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Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Science, 1988A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus , greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity ...
R K, Saiki +7 more
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Primers for Animal Mitochondrial DNA: The Importance of Species-Specific Primers
1998In this chapter we outline the procedure for designing oligonucleotide primers for the direct-sequencing of in vitro amplified animal mitochondrial DNA (mtDNA). Although this chapter deals with animal mtDNA in particular, the approach is of course applicable to any gene, nuclear or mitochondrial. There are four main ‘steps’:identification of a suitable
Palsboll, Per, ARCTANDER, P
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Blood, 2015
Abstract RNA-guided Cas9 nucleases based on the prokaryotic CRISPR-Cas systems provide an unprecedented ease in being able to edit the genomes of diverse organisms. As a single unifying factor capable of co-localizing RNA, DNA, and protein, I believe tools and techniques based on Cas9 will grant exquisite control over cellular ...
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Abstract RNA-guided Cas9 nucleases based on the prokaryotic CRISPR-Cas systems provide an unprecedented ease in being able to edit the genomes of diverse organisms. As a single unifying factor capable of co-localizing RNA, DNA, and protein, I believe tools and techniques based on Cas9 will grant exquisite control over cellular ...
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Introduction: DNA Basics—A Primer on DNA
2020To better understand the contents of this book, the lay reader needs to know some basic facts about DNA, and the aim of the following brief introduction is to provide such a necessary background. The reader may also bump in the next chapters into some unusual terms so the Glossary at the end of this book should help him/her to understand the meaning of
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Effect of Irradiation of the Primer DNA on the Incorporation of Precursors
International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1961SummaryThe rate of enzymatic incorporation of 3H-labelled thymidine into primer DNA was studied after the DNA had been irradiated with doses up to 360 000 roentgens of gamma-rays. Non-irradiated DNA-synthesizing enzyme obtained from regenerating rat-liver was used together with DNA prepared by the method of Kay, Simmons and Dounce (1952) in the ...
C M, WHEELER, S, OKADA
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Universal COI primers for DNA barcoding amphibians
Molecular Ecology Resources, 2011AbstractDNA barcoding is a proven tool for the rapid and unambiguous identification of species, which is essential for many activities including the vouchering tissue samples in the genome 10K initiative, genealogical reconstructions, forensics and biodiversity surveys, among many other applications.
Jing, Che +7 more
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Ribosomal DNA Probes and Primers
1998Ribosomal DNA (rDNA) is the region of the genome coding for the RNA component of ribosomes. The ubiquity and conservation of rDNA sequences has made these DNA regions important tools for reconstructing phylogenetic relationships. In eukaryotes, rDNA sequences may be found in the nucleus and organelles.
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SERS Primers and Their Mode of Action for Pathogen DNA Detection
Analytical Chemistry, 2013SERS primers have been used to directly detect specific PCR products utilizing the difference in adsorption of single-stranded and double-stranded DNA onto nanoparticle surfaces. Herein, seven parameters important for improved positive SERS assays for real applications were investigated.
van Lierop, Danny +3 more
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