Results 271 to 280 of about 1,480,527 (305)
Some of the next articles are maybe not open access.
DNA sequencing by synthesis with degenerate primers
Journal of Genetics and Genomics, 2008The degenerate primer-based sequencing was developed by a synthesis method (DP-SBS) for high-throughput DNA sequencing, in which a set of degenerate primers are hybridized on the arrayed DNA templates and extended by DNA polymerase on microarrays.
Chao, Tang +3 more
openaire +2 more sources
DNA amplification fingerprinting using arbitrary oligonucleotide primers
Applied Biochemistry and Biotechnology, 1993DNA amplification fingerprinting (DAF) is a strategy for genetic typing and mapping that uses one or more very short (> or = 5 nt) arbitrary oligonucleotides to direct the enzymatic amplification of discrete portions of a DNA template resulting in a spectrum of products characteristic of the DNA starting material.
G, Caetano-Anollés, B J, Bassam
openaire +2 more sources
Fluorescent labeling of a DNA sequencing primer
DNA Sequence, 1993Several oligonucleotides containing one to four fluorescein labels in various positions were synthesized and the fluorescence intensity and thermal stability of the duplex forms with their complementary sequences were measured. Oligomers that contain two fluorescein molecules, one attached at the 5' terminus and the other at an internal phosphate, were
J, Mineno +3 more
openaire +2 more sources
Introduction: DNA Basics—A Primer on DNA
2020To better understand the contents of this book, the lay reader needs to know some basic facts about DNA, and the aim of the following brief introduction is to provide such a necessary background. The reader may also bump in the next chapters into some unusual terms so the Glossary at the end of this book should help him/her to understand the meaning of
openaire +1 more source
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Science, 1988A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus , greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity ...
R K, Saiki +7 more
openaire +2 more sources
DNA Synthesis by Primer Exchange Reaction Cascades
ChemBioChem, 2018Swap and extend: The autonomous synthesis of single-stranded DNA molecules of arbitrary size and sequence composition can easily be achieved by primer exchange reaction (PER) cascades, in which the sequential polymerase-mediated extension of DNA primers is guided by catalytic hairpins.
openaire +2 more sources
“Primer” in DNA Polymerase Reactions
1963Publisher Summary This chapter reviews two degrees of primer activation. The first concerns the secondary structure of the DNA molecule, and for the calf thymus and phage-induced E. coli polymerase this type of activation, typically produced by heat, acid, or alkaline denaturation, results in rate increases of one to two orders of magnitude.
openaire +1 more source
DNA Extraction and Primer Selection
2013Talk regarding pitfalls in DNA extraction and 16S amplicon primer choice when performing community analysis of complex microbial communities. The talk was a part of Workshop 2 "Principles, Potential, and Limitations of Novel Molecular Methods in Water Engineering; from Amplicon Sequencing to -omics Methods" given at the MEWE 2013 conference in Ann ...
Karst, Søren Michael; id_orcid 0000-0002-0233-160X +3 more
openaire +2 more sources
Ribosomal DNA Probes and Primers
1998Ribosomal DNA (rDNA) is the region of the genome coding for the RNA component of ribosomes. The ubiquity and conservation of rDNA sequences has made these DNA regions important tools for reconstructing phylogenetic relationships. In eukaryotes, rDNA sequences may be found in the nucleus and organelles.
openaire +1 more source
Designing PCR Primer for DNA Methylation Mapping
2007DNA methylation is an epigenetic mechanism of gene regulation, and aberrant methylation has been associated with various types of diseases, especially cancers. Detection of DNA methylation has thus become an important approach for studying gene regulation and has potential diagnostic application.
openaire +2 more sources