Results 81 to 90 of about 3,903,497 (411)

The Saccharomyces cerevisiae amino acid transporter Lyp1 has a broad substrate spectrum

FEBS Letters, EarlyView.
In Saccharomyces cerevisiae, Yeast Amino acid Transporter family members mediate the import of amino acids, ranging from substrate specialists to generalists. Here, we show that the specialist transporter, Lyp1, has a broader substrate spectrum than previously described, with affinity constants spanning from micromolar to millimolar.
Foteini Karapanagioti, Sebastian Obermaier, Dirk J. Slotboom, Bert Poolman   +3 more
wiley   +1 more source

miRNA‐29 regulates epidermal and mesenchymal functions in skin repair

FEBS Letters, EarlyView.
miRNA‐29 inhibits cell‐to‐cell and cell‐to‐matrix adhesion by silencing mRNA targets. Adhesion is controlled by complex interactions between many types of molecules coded by mRNAs. This is crucial for keeping together the layers of the skin and for regenerating the skin after wounding.
Lalitha Thiagarajan, Rosa Sanchez‐Alvarez, Chiho Kambara, Poojitha Rajasekar, Yuluang Wang, François Halloy, Jonathan Hall, Hans‐Jürgen Stark, Iris Martin, Petra Boukamp, Svitlana Kurinna   +10 more
wiley   +1 more source

Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis

Brazilian Journal of Infectious Diseases, 2011
Objective: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous ...
Diego Molina de Oliveira, M.D., Maria Valdrinez Campana Lonardoni, M.D.Ph.D., Ueslei Teodoro, Thais Gomes Verzignassi Silveira   +3 more
doaj  

Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens [PDF]

, 2003
BACKGROUND: We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
Blackburn, Gary F, Chan, Vivian, Chen, Yin-Peng, Farkas, Daniel H, Miller, Donna L, Reeves, William C, Unger, Elizabeth R, Vernon, Suzanne D   +7 more
core   +3 more sources

Environmental DNA sequencing primers for eutardigrades and bdelloid rotifers [PDF]

BMC Ecology, 2009
The time it takes to isolate individuals from environmental samples and then extract DNA from each individual is one of the problems with generating molecular data from meiofauna such as eutardigrades and bdelloid rotifers. The lack of consistent morphological information and the extreme abundance of these classes makes morphological identification of ...
Jeremy Whiting, Elizabeth K. Costello, Steve K. Schmidt, Andrew P. Martin, Kristen R. Freeman, Michael S. Robeson, Byron J. Adams   +6 more
openaire   +4 more sources

A cellular system to study responses to a collision between the transcription complex and a protein‐bound nick in the DNA template

FEBS Letters, EarlyView.
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring, Morten Roedgaard, Camilla Myrup Holst, Helene Christensen, Birgitta R. Knudsen, Lotte Bjergbaek, Anni Hangaard Andersen   +6 more
wiley   +1 more source

Effectiveness of annealing blocking primers versus restriction enzymes for characterization of generalist diets: unexpected prey revealed in the gut contents of two coral reef fish species. [PDF]

PLoS ONE, 2013
Characterization of predator-prey interactions is challenging as researchers have to rely on indirect methods that can be costly, biased and too imprecise to elucidate the complexity of food webs.
Matthieu Leray, Natalia Agudelo, Suzanne C Mills, Christopher P Meyer   +3 more
doaj   +1 more source

Standard and Light-Cycler PCR methods for animal DNA species detection in animal feedstuffs [PDF]

, 2008
In this work four species-specific primers and probes were designed and evaluated for the detection and quantification of bovine, ovine, swine and chicken mitochondrial DNA in feeds.
Ajmone_Marsan, P, Chegdani, F, Chiappini, B, Faggionato, E, Fontana, C, Frezza, D, Giambra, V, Lanni, L, Losio, N, Maccabiani, G, Saccares, S, Vaccari, G, von Holst, C   +12 more
core   +1 more source

Template-primer analogs as substrates for DNA polymerase

Nucleic Acids Research, 1987
In order to gain more understanding about the mode of action of DNA polymerase, eight related partially self-complementary "hairpin" shaped oligodeoxynucleotides were prepared. Four of the oligomers contained either 1-beta-D-arabinofuranosyluracil (ara-U) or 1-beta-D-2'deoxyxylofuranosylthymine (dxT) nucleoside analogs at their 3' termini.
Peter Ng, Thomas R. Webb, Parkash Jhurani   +2 more
openaire   +4 more sources

One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes

Persoonia, 2015
The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode.
J. B. Stielow, C. Lévesque, K. Seifert, W. Meyer, L. Iriny, D. Smits, R. Renfurm, G. Verkley, M. Groenewald, D. Chaduli, A. Lomascolo, S. Welti, L. Lesage-Meessen, A. Favel, A. Al‐Hatmi, U. Damm, N. Yilmaz, J. Houbraken, L. Lombard, W. Quaedvlieg, M. Binder, L. Vaas, D. Vu, Andrey M. Yurkov, D. Begerow, O. Roehl, M. Guerreiro, A. Fonseca, K. Samerpitak, A. V. van Diepeningen, S. Dolatabadi, L. Moreno, S. Casaregola, S. Mallet, N. Jacques, L. Roscini, E. Egidi, C. Bizet, D. Garcia-Hermoso, M. P. Martín, S. Deng, J. Groenewald, T. Boekhout, Z. W. de Beer, I. Barnes, T. Duong, M. Wingfield, G. de Hoog, P. Crous, C. Lewis, S. Hambleton, T. Moussa, H. Al-Zahrani, O. Almaghrabi, G. Louis-Seize, R. Assabgui, W. McCormick, G. Omer, K. Dukik, G. Cardinali, U. Eberhardt, M. de Vries, V. Robert   +62 more
semanticscholar   +1 more source