Results 81 to 90 of about 4,042,809 (333)

Development of EST derived-SSRs of Theobroma grandiflorum for molecular breeding : S04P05 [PDF]

open access: yes, 2013
Simple sequence repeats (SSRs) markers are widely used in breeding programs, and SSRs associated to expressed sequence tags (ESTs) could be quite efficient in the selection of genotypes of interest.
Alves, Rafael Moyses   +7 more
core  

Family-specific degenerate primer design: a tool to design consensus degenerated oligonucleotides [PDF]

open access: yes, 2013
Designing degenerate PCR primers for templates of unknown nucleotide sequence may be a very difficult task. In this paper, we present a new method to design degenerate primers, implemented in family-specific degenerate primer design (FAS-DPD) computer ...
Borio, Cristina Silvia   +5 more
core   +3 more sources

Function‐driven design of a surrogate interleukin‐2 receptor ligand

open access: yesFEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang   +9 more
wiley   +1 more source

Mechanisms of parasite‐mediated disruption of brain vessels

open access: yesFEBS Letters, EarlyView.
Parasites can affect the blood vessels of the brain, often causing serious neurological problems. This review explains how different parasites interact with and disrupt these vessels, what this means for brain health, and why these processes matter. Understanding these mechanisms may help us develop better ways to prevent or treat brain infections in ...
Leonor Loira   +3 more
wiley   +1 more source

The Effects of DNA Extraction Kits and Primers on Prokaryotic and Eukaryotic Microbial Community in Freshwater Sediments [PDF]

open access: gold, 2022
Zihan Shi   +7 more
openalex   +1 more source

One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes

open access: yesPersoonia, 2015
The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode.
J. B. Stielow   +62 more
semanticscholar   +1 more source

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

open access: yesFEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf   +6 more
wiley   +1 more source

Constructing a DNA ladder Range for Lambda Phage by multiplex PCR

open access: yesIranian Journal of Microbiology, 2010
Background and Objectives: DNA ladder contains DNA fragments of different length but with known size, used to determine the size of unknown DNA molecules. Different DNA ladders are available for expected DNA length.
R Gopalakrishnan, S Joseph, S Sellappa
doaj  

Development of environmental DNA metabarcoding primers for marine mollusks and comparison with published primers

open access: yesBMC Ecology and Evolution
Monitoring mollusk biodiversity is a great challenge due to their large diversity and broad distribution. Environmental DNA (eDNA) technology is increasingly applied for biodiversity monitoring, but relevant studies on marine mollusks are still limited ...
Xiaojing Shi   +3 more
doaj   +1 more source

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