Comparison of Single-Stranded DNA Probes Conjugated with Magnetic Particles for Trans-Cleavage in Cas12a-Based Biosensors [PDF]
Biosensors, 2023 Biosensors based on endonuclease Cas12 provide high specificity in pathogen detection. Sensitive detection using Cas12-based assays can be achieved using trans-cleaved DNA probes attached to simply separated carriers, such as magnetic particles (MPs ...
Aleksandr V. Ivanov +4 more
doaj +2 more sources
A novel cassette method for probe evaluation in the designed biochips. [PDF]
PLoS ONE, 2014 A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Vitaly Zinkevich +7 more
doaj +5 more sources
DNA probes that store mechanical information reveal transient piconewton forces applied by T cells. [PDF]
Proc Natl Acad Sci U S A, 2019 Significance To defend against cancer and viral infections, the T cell receptor (TCR) must recognize antigens on the surface of target cells. TCR antigen recognition involves the transmission of forces which are often weak, infrequent, and short lived ...
Ma R +6 more
europepmc +2 more sources
Quantitative Analysis of Nucleic Acid Hybridization on Magnetic Particles and Quantum Dot-Based Probes [PDF]
Sensors, 2009 In the present study we describe sandwich design hybridization probes consisting of magnetic particles (MP) and quantum dots (QD) with target DNA, and their application in the detection of avian influenza virus (H5N1) sequences. Hybridization of 25-, 40-,
Alexey Dan Chin Yu +4 more
doaj +6 more sources
Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens [PDF]
BMC Infectious Diseases, 2003 Background We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
Miller Donna L +7 more
doaj +4 more sources
A Feature Selection Algorithm to Compute Gene Centric Methylation from Probe Level Methylation Data. [PDF]
PLoS ONE, 2016 DNA methylation is an important epigenetic event that effects gene expression during development and various diseases such as cancer. Understanding the mechanism of action of DNA methylation is important for downstream analysis.
Brittany Baur, Serdar Bozdag
doaj +6 more sources
LNA probes substantially improve the detection of bacterial endosymbionts in whole mount of insects by fluorescent
BMC Microbiology, 2012 Background Detection of unculturable bacteria and their localization in the host, by fluorescent in-situ hybridization (FISH), is a powerful technique in the study of host-bacteria interaction.
Priya Natarajan +2 more
doaj +3 more sources
Sensors, 2010 The CombiMatrix microarray with 12,544 electrodes supports in situ electrochemical synthesis of user-defined DNA probes. As an alternative, we immobilized commercially synthesized DNA probes on individual electrodes coated with electropolymerized ...
David Danley +5 more
doaj +2 more sources
A repetitive probe for FISH analysis of bovine interphase nuclei [PDF]
Genetics Selection Evolution, 2000 The purpose of this study was to generate repetitive DNA sequence probes for the analysis of interphase nuclei by fluorescent in situ hybridisation (FISH).
Cribiu Edmond +5 more
doaj +6 more sources
Embryo sexing methods in bovine and its application in animal breed
Journal of Animal Reproduction and Biotechnology, 2022 The ability to determine the sex of bovine embryos before the transfer is advantageous in livestock management, especially in dairy production, where female calves are preferred in milk industry.
Shelema Kelbessa Bora
doaj +1 more source