A novel cassette method for probe evaluation in the designed biochips. [PDF]
PLoS ONE, 2014 A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Vitaly Zinkevich +7 more
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Comparison of Single-Stranded DNA Probes Conjugated with Magnetic Particles for Trans-Cleavage in Cas12a-Based Biosensors [PDF]
Biosensors, 2023 Biosensors based on endonuclease Cas12 provide high specificity in pathogen detection. Sensitive detection using Cas12-based assays can be achieved using trans-cleaved DNA probes attached to simply separated carriers, such as magnetic particles (MPs ...
Aleksandr V. Ivanov +4 more
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Biosensing using hairpin DNA probes [PDF]
Reviews in Analytical Chemistry, 2015 Hairpin DNA probes (HDPs) are specially designed single-stranded DNA and have excellent sensing specificity. The past decade has witnessed the fast development of HDP-based biosensors due to the tremendous applications in biology, medicine, environmental
Huang Jiahao, Wu Jueqi, Li Zhigang
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Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens [PDF]
BMC Infectious Diseases, 2003 Background We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
Miller Donna L +7 more
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Hapten-labeled DNA probes can be stored and used in fluorescence in situ hybridization for decades [PDF]
Frontiers in GeneticsIn molecular cytogenetics, fluorescence in situ hybridization (FISH) is the main technique used. In both research and diagnostics, FISH depends on well-defined and mapped DNA probes that produce brilliant signals with minimal background, visible in ...
Thomas Liehr +2 more
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A Feature Selection Algorithm to Compute Gene Centric Methylation from Probe Level Methylation Data. [PDF]
PLoS ONE, 2016 DNA methylation is an important epigenetic event that effects gene expression during development and various diseases such as cancer. Understanding the mechanism of action of DNA methylation is important for downstream analysis.
Brittany Baur, Serdar Bozdag
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Automated High-Throughput Probe Production for DNA Microarray Analysis [PDF]
BioTechniques, 2003 DNA microarrays have become an established tool for gene expression profiling. Construction of these microarrays using immobilized cDNAs is a common experimental strategy.
P.R. Hoyt +5 more
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An electrophoretic mobility shift assay with chemiluminescent readout to evaluate DNA-targeting oligonucleotide-based probes. [PDF]
PLoS ONEA comprehensive and user-friendly method for evaluating recognition of double-stranded DNA (dsDNA) targets by oligonucleotide-based probes is presented. Thus, dsDNA-targeting probes such as single-stranded locked nucleic acids (LNAs) and double-stranded ...
Michaela E Everly +3 more
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Embryo sexing methods in bovine and its application in animal breed
Journal of Animal Reproduction and Biotechnology, 2022 The ability to determine the sex of bovine embryos before the transfer is advantageous in livestock management, especially in dairy production, where female calves are preferred in milk industry.
Shelema Kelbessa Bora
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Factors Impacting Invader-Mediated Recognition of Double-Stranded DNA
Molecules, 2022 The development of chemically modified oligonucleotides enabling robust, sequence-unrestricted recognition of complementary chromosomal DNA regions has been an aspirational goal for scientists for many decades.
Caroline P. Shepard +3 more
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