Probing the Mechanical Unzipping of DNA [PDF]
Physical Review Letters, 2006 A study of the micromechanical unzipping of DNA in the framework of the Peyrard-Bishop-Dauxois model is presented. We introduce a Monte Carlo technique that allows accurate determination of the dependence of the unzipping forces on unzipping speed and temperature. Our findings agree quantitatively with experimental results for homogeneous DNA, and for $
Kim Ø. Rasmussen +3 more
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A novel cassette method for probe evaluation in the designed biochips [PDF]
, 2014 A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Al-humam, Abdulmohsen A. +7 more
core +8 more sources
Probing Allostery Through DNA [PDF]
Science, 2013 Allostery Across DNA Proteins, such as transcription factors and RNA polymerase, bind close to each other on DNA and their function is coordinated. Kim et al. (p. 816 ; see the Perspective by Crothers
Yujie Sun +13 more
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Fluorescent Probes of DNA Repair [PDF]
ACS Chemical Biology, 2017 DNA repair is now understood to play a key role in a variety of disease states, most notably cancer. Tools for studying DNA have typically relied on traditional biochemical methods which are often laborious and indirect. Efforts to study the biology and therapeutic relevance of DNA repair pathways can be limited by such methods.
David L. Wilson, Eric T. Kool
openaire +2 more sources
Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]
, 1992 Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke +33 more
core +1 more source
Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens [PDF]
, 2003 BACKGROUND: We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
Blackburn, Gary F +7 more
core +3 more sources
Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]
, 1992 Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Brooks-Wilson +15 more
core +1 more source
Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy [PDF]
, 1995 Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single ...
Jalocha, A. +5 more
core +8 more sources
A cellulose-based bioassay for the colorimetric detection of pathogen DNA [PDF]
, 2014 Cellulose-paper-based colorimetric bioassays may be used at the point of sampling without sophisticated equipment. This study reports the development of a colorimetric bioassay based on cellulose that can detect pathogen DNA.
Goyal, Madhu +3 more
core +1 more source
Electrically Guided DNA Immobilization and Multiplexed DNA Detection with Nanoporous Gold Electrodes. [PDF]
, 2018 Molecular diagnostics have significantly advanced the early detection of diseases, where the electrochemical sensing of biomarkers (e.g., DNA, RNA, proteins) using multiple electrode arrays (MEAs) has shown considerable promise.
Daggumati, Pallavi +3 more
core +3 more sources