In the present study we describe sandwich design hybridization probes consisting of magnetic particles (MP) and quantum dots (QD) with target DNA, and their application in the detection of avian influenza virus (H5N1) sequences. Hybridization of 25-, 40-,
Alexey Dan Chin Yu +4 more
doaj +1 more source
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye.
W. Gregory Cox, Victoria L. Singer
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Electrochemical detection of the toxic dinoflagellate Alexandrium ostenfeldii with a DNA-biosensor [PDF]
The steady rise of observations of harmful or toxic algal blooms throughout the world in the past decades constitute a menace for coastal ecosystems and human interests.
Huljic, S. +3 more
core +1 more source
A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome-specific DNA libraries and YAC clones [PDF]
The identification of marker chromosomes in clinical and tumor cytogenetics by chromosome banding analysis can create problems. In this study, we present a strategy to define minute chromosomal rearrangements by multicolor fluorescence in situ ...
A Kallioniemi +31 more
core +1 more source
Multicolor chromosome bar codes [PDF]
Chromosome bar codes are multicolor banding patterns produced by fluorescence in situ hybridization (FISH) with differentially labeled and pooled sub-regional DNA probes.
Müller, Stefan, Wienberg, Johannes
core +1 more source
DNA fingerprinting and cloning of hypervariable minisatellite repeats in salmonids [PDF]
We used heterologous jeffreys' 33.6 core sequence and microsatellites (CAC)(5) and (CA)(12) as probes and compared them with probes based on the minisatellite sequences from tilapia (Oreochromis niloticus) and Atlantic salmon (Salmo salar) in ...
Leung, FCC +3 more
core +1 more source
Sequence-specific cleavage of double helical DNA by triple helix formation [PDF]
Homopyrimidine oligodeoxyribonucleotides with EDTA-Fe attached at a single position bind the corresponding homopyrimidine-homopurine tracts within large double-stranded DNA by triple helix formation and cleave at that site.
Dervan, Peter B., Moser, Heinz E.
core +1 more source
DNA tetrahedron for fluorescence anisotropy of intercalators
BackgroundThe fluorescence anisotropy of intercalative dye plays important roles in nucleic acid analysis, the construction of fluorescent probes, and biosensing.
HUANG Qiuling +4 more
doaj +1 more source
DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus [PDF]
We used three short repetitive nucleotide sequences [(GTG)5, (TAC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted Citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic ...
Asif, M +7 more
core +2 more sources
Sequence-specific detection of nucleic acids has been intensively studied in the field of molecular diagnostics. In particular, the detection and analysis of single-nucleotide polymorphisms (SNPs) is crucial for the identification of disease-causing ...
Gil Tae Hwang
doaj +1 more source

