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Fluorescent silver nanoclusters as DNA probes.

Nanoscale, 2013
Fluorescent silver nanoclusters (few atoms, quantum sized) have attracted much attention as promising substitutes for conventional fluorophores. Due to their unique environmental sensitivities, new fluorescent probes have been developed based on silver ...
J. Obliosca, Cong Liu, H. Yeh
semanticscholar   +1 more source

Absolute and direct microRNA quantification using DNA-gold nanoparticle probes.

Journal of the American Chemical Society, 2014
DNA-gold nanoparticle probes are implemented in a simple strategy for direct microRNA (miRNA) quantification. Fluorescently labeled DNA-probe strands are immobilized on PEGylated gold nanoparticles (AuNPs).
Federica Degliangeli   +4 more
semanticscholar   +1 more source

Characterization of DNA Probes Immobilized on Gold Surfaces

, 1997
We have characterized thiol-derivatized, single-stranded DNA (5‘-HS-(CH2)6-CAC GAC GTT GTA AAA CGA CGG CCA G-3‘, abbreviated HS-ssDNA) attached to gold via a sulfur−gold linkage using X-ray photoelectron spectroscopy (XPS), ellipsometry, and 32P ...
T. Herne, M. Tarlov
semanticscholar   +1 more source

Internal Labeling of DNA Probes

2003
Ralph Rapley and BimalD.M. Theophilus, 'Internal labeling of DNA Probes' in R. Rapley and B. D. M. Theophilus, eds., PCR Mutation Detection Protocols (Totowa: Humana Press 2002) ISBN: 978-0-89603-617-8, eISBN: 978-1-59259-273-9, DOI: 10.1385/1-59259-273-2:013 © 2002 Humana Press Inc., Totowa, NJ.
Bimal D. M. Theophilus, Ralph Rapley
openaire   +3 more sources

Array-Based Electrical Detection of DNA with Nanoparticle Probes

Science, 2002
A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target-probe binding events.
So-Jung Park, T. Taton, C. Mirkin
semanticscholar   +1 more source

Diagnostic Applications of DNA Probes [PDF]

open access: possibleInfection Control & Hospital Epidemiology, 1991
The use of recombinant DNA technology to develop specific molecular (usually DNA) probes has provided medical and veterinary diagnostic laboratories with powerful new tools to enhance the diagnosis of infectious diseases, genetic disorders, and malignancies, as well as more sensitive and specific means to accomplish such tasks as tissue typing and ...
openaire   +2 more sources

Graphene oxide-protected DNA probes for multiplex microRNA analysis in complex biological samples based on a cyclic enzymatic amplification method.

Chemical Communications, 2012
Based on graphene oxide-protected DNA probes, we have developed a cyclic enzymatic amplification method for sensitive miRNA detection in complex biological samples.
Liang Cui   +6 more
semanticscholar   +1 more source

Probing RNA Translocases with DNA

2014
For some helicases, it is possible to investigate RNA translocase activity on DNA substrates because the enzyme acts on both substrates. Potassium permanganate (KMnO4) footprinting is a method used to chemically probe the conformation of DNA as well as the binding of proteins.
Kimberly A. Reynolds   +2 more
openaire   +3 more sources

Design aspects of bright red emissive silver nanoclusters/DNA probes for microRNA detection.

ACS Nano, 2012
The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated ...
Pratik Shah   +8 more
semanticscholar   +1 more source

[7] Probes of DNA structure

1992
Publisher Summary This chapter discusses the structure of DNA by enzymes and chemicals probing. Enzyme and chemical probing experiments have generated important insight into a number of altered DNA structures, including cruciform structures, left-handed Z-DNA, A tract-containing curved DNA, H-triplexes, G-tetrad structures, and parallel-stranded DNA,
openaire   +3 more sources

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