Results 41 to 50 of about 763,201 (203)

Microarray-based ultra-high resolution discovery of genomic deletion mutations [PDF]

, 2014
BACKGROUND: Oligonucleotide microarray-based comparative genomic hybridization (CGH) offers an attractive possible route for the rapid and cost-effective genome-wide discovery of deletion mutations.
Baban, D., Belfield, E., Brown, C., Gan, X., Harberd, N., Jiang, C., Mithani, A., Mott, R., Ragoussis, J.   +8 more
core   +3 more sources

Fluorescent Probes of DNA Repair [PDF]

ACS Chemical Biology, 2017
DNA repair is now understood to play a key role in a variety of disease states, most notably cancer. Tools for studying DNA have typically relied on traditional biochemical methods which are often laborious and indirect. Efforts to study the biology and therapeutic relevance of DNA repair pathways can be limited by such methods.
David L. Wilson, Eric T. Kool
openaire   +2 more sources

Long Oligonucleotide Arrays on Nylon for Large-Scale Gene Expression Analysis

BioTechniques, 2002
To date, most studies of multigenic expression patterns by long DNA array have used DNA fragments as probes. These probes are usually obtained as PCR products, and this represents a time-consuming and error-prone approach, requiring strict quality ...
M. El Atifi, I. Dupré, B. Rostaing, E.M. Chambaz, A.L. Benabid, F. Berger   +5 more
doaj   +1 more source

Use of a Multiplexed CMOS Microarray to Optimize and Compare Oligonucleotide Binding to DNA Probes Synthesized or Immobilized on Individual Electrodes

Sensors, 2010
The CombiMatrix microarray with 12,544 electrodes supports in situ electrochemical synthesis of user-defined DNA probes. As an alternative, we immobilized commercially synthesized DNA probes on individual electrodes coated with electropolymerized ...
David Danley, Wanda Lyon, John Cooper, Jodi Wilmoth, Nina Yazvenko, Karl Maurer   +5 more
doaj   +1 more source

Characterization of two marker chromosomes in a patient with acute nonlymphocytic leukemia by two-color fluorescence in situ hybridization [PDF]

, 1993
A patient with acute nonlymphocytic leukemia (ANLL), M5b according to French-American-British (FAB) classification, showed monosomy 16, an extra 1p−, and a 21q+. These derivative chromosomes could not be defined by GTG-banding.
Anna Jauch, Brothman, Burke, Christoph Lengauer, Collins, Cooke, Cremer, Cremer, Jauch, Klever, Lengauer, Lengauer, Lengauer, Lichter, Lichter, Marosi, Masafumi Taniwaki, Michael R. Speicher, Moyzis, Pinkel, Pinkel, Rowley, Selleri, Smit, Speleman, Susanne Popp, Thomas Cremer   +26 more
core   +1 more source

Chemically programmable bacterial probes for the recognition of cell surface proteins

Materials Today Bio, 2023
Common methods to label cell surface proteins (CSPs) involve the use of fluorescently modified antibodies (Abs) or small-molecule-based ligands. However, optimizing the labeling efficiency of such systems, for example, by modifying them with additional ...
Pragati K. Prasad, Noa Eizenshtadt, Inna Goliand, Liat Fellus-Alyagor, Roni Oren, Ofra Golani, Leila Motiei, David Margulies   +7 more
doaj   +1 more source

Sequence Heterogeneity Accelerates Protein Search for Targets on DNA [PDF]

, 2015
The process of protein search for specific binding sites on DNA is fundamentally important since it marks the beginning of all major biological processes. We present a theoretical investigation that probes the role of DNA sequence symmetry, heterogeneity
Alberts B., Alexey A. Shvets, Anatoly B. Kolomeisky, Dill K. A., Lodish H., Phillips R.   +5 more
core   +2 more sources

DNA probe for Aeromonas salmonicida [PDF]

Applied and Environmental Microbiology, 1992
A DNA fragment that is specific to Aeromonas salmonicida has been isolated from a genomic DNA library by differential hybridization. The specificity of this fragment as a DNA probe for A. salmonicida was shown by hybridization against reference strains and clinical isolates of A. salmonicida, related aeromonads, and species from several other bacterial
M, Hiney, M T, Dawson, D M, Heery, P R, Smith, F, Gannon, R, Powell   +5 more
openaire   +2 more sources

Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization

Nature Communications, 2019
Optimisation of nucleic acid probes and blocker strands can be laborious. Here the authors construct a theoretical model of competitive DNA hybridisation to design DNA probes for optimisation-free mutation detection.
Xin Chen, Na Liu, Liquan Liu, Wei Chen, Na Chen, Meng Lin, Jiaju Xu, Xing Zhou, Hongbo Wang, Meiping Zhao, Xianjin Xiao   +10 more
doaj   +1 more source

Intra- and Interspecies Analyses of the Carcinoembryonic Antigen (CEA) Gene Family Reveal Independent Evolution in Primates and Rodents [PDF]

, 1989
Various rodent and primate DNAs exhibit a stronger intra- than interspecies cross-hybridization with probes derived from the N-terminal domain exons of human and rat carcinoembryonic antigen (CEA)-like genes.
Rudert, Fritz, Thompson, John A., Zimmermann, Wolfgang   +2 more
core   +1 more source