A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome-specific DNA libraries and YAC clones [PDF]
The identification of marker chromosomes in clinical and tumor cytogenetics by chromosome banding analysis can create problems. In this study, we present a strategy to define minute chromosomal rearrangements by multicolor fluorescence in situ ...
A Kallioniemi+31 more
core +1 more source
DNA fingerprinting and cloning of hypervariable minisatellite repeats in salmonids [PDF]
We used heterologous jeffreys' 33.6 core sequence and microsatellites (CAC)(5) and (CA)(12) as probes and compared them with probes based on the minisatellite sequences from tilapia (Oreochromis niloticus) and Atlantic salmon (Salmo salar) in ...
Leung, FCC+3 more
core +1 more source
DNA Stable-Isotope Probing (DNA-SIP) [PDF]
DNA stable-isotope probing (DNA-SIP) is a powerful technique for identifying active microorganisms that assimilate particular carbon substrates and nutrients into cellular biomass. As such, this cultivation-independent technique has been an important methodology for assigning metabolic function to the diverse communities inhabiting a wide range of ...
Eric A. Dunford, Josh D. Neufeld
openaire +3 more sources
DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications
Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes.
Vladimir V. Didenko
doaj +1 more source
Multicolor chromosome bar codes [PDF]
Chromosome bar codes are multicolor banding patterns produced by fluorescence in situ hybridization (FISH) with differentially labeled and pooled sub-regional DNA probes.
Müller, Stefan, Wienberg, Johannes
core +1 more source
Probing for DNA methylation with a voltammetric DNA detector [PDF]
Label-free electrochemical method for the detection of prostate cancer using a DNA biomarker.
Amir Saheb+2 more
openaire +3 more sources
Biosensing using hairpin DNA probes
Hairpin DNA probes (HDPs) are specially designed single-stranded DNA and have excellent sensing specificity. The past decade has witnessed the fast development of HDP-based biosensors due to the tremendous applications in biology, medicine, environmental
Huang Jiahao, Wu Jueqi, Li Zhigang
doaj +1 more source
Evaluation of dual-labeled fluorescent DNA probe purity versus performance in real-time PCR
Real-time PCR technology using dual-labeled fluorescent oligonucleotide probes allows for sensitive, specific, and quantitative determination of mRNA or DNA targets.
Anthony T. Yeung+3 more
doaj +1 more source
Sequence-specific cleavage of double helical DNA by triple helix formation [PDF]
Homopyrimidine oligodeoxyribonucleotides with EDTA-Fe attached at a single position bind the corresponding homopyrimidine-homopurine tracts within large double-stranded DNA by triple helix formation and cleave at that site.
Dervan, Peter B., Moser, Heinz E.
core +1 more source
DNA multi-bit non-volatile memory and bit-shifting operations using addressable electrode arrays and electric field-induced hybridization. [PDF]
DNA has been employed to either store digital information or to perform parallel molecular computing. Relatively unexplored is the ability to combine DNA-based memory and logical operations in a single platform.
Heller, Michael J+3 more
core +3 more sources