FEBS Letters, EarlyView.Single‐cell RNA sequencing reveals an opposite role of SLPI in basal tumors based on metastatic spread, along with shared activation of specific regulons in cancer cells and mature luminal lactocytes, as well as downregulation of MALAT1 and NEAT1 in the latter.
Pietro Ancona +4 more
wiley +1 more source
Genetic architecture of the Sindhi Indian population: a 19X-STR forensic analysis
BMC GenomicsThe X chromosome accounts for approximately 5% of the human genome and serves as a valuable marker in forensic and population genetics due to its uneven and asymmetrical pattern of inheritance.
Khushboo Gautam +6 more
doaj +1 more source
Systematic evaluation of genome-wide methylated DNA enrichment using a CpG island array
BMC Genomics, 2011 Background Recent progress in high-throughput technologies has greatly contributed to the development of DNA methylation profiling. Although there are several reports that describe methylome detection of whole genome bisulfite sequencing, the high cost ...
Zhao Qian +6 more
doaj +1 more source
Systems biology in inflammatory bowel diseases [PDF]
, 2014 Purpose of review: Ulcerative colitis (UC) and Crohn’s Disease (CD) are the two predominant types of inflammatory bowel disease (IBD), affecting over 1.4 million individuals in the US.
Iliopoulos, D, Koukos, G, Polytarchou, C
core +1 more source
Cancer cell death induced by the NAD antimetabolite Vacor discloses the antitumor potential of SARM1
FEBS Letters, EarlyView.Vacor, a compound converted into the toxic metabolite Vacor adenine dinucleotide (VAD) by the nicotinamide salvage pathway enzymes NAMPT and NMNAT2, exhibits antitumor activity by inducing rapid and complete NAD depletion. We report that Vacor toxicity is limited to cell lines expressing high levels of SARM1, a NAD glycohydrolase.
Giuseppe Ranieri +5 more
wiley +1 more source
DNA-Based Kinship Analysis [PDF]
, 2008 Relatedness between individuals and groups can be investigated using DNA markers. A child’s DNA profile is a combination of alleles passed down from the father and mother.
Maguire, Christopher, Woodward, Michael
core
Establishment and characterization of two human breast carcinoma cell lines by spontaneous immortalization: Discordance between Estrogen, Progesterone and HER2/neu receptors of breast carcinoma tissues with derived cell lines [PDF]
, 2012 Background: Breast cancer is one of the most common cancers among women throughout the world. Therefore, established cell lines are widely used as in vitro experimental models in cancer research.Methods: Two continuous human breast cell lines, designated
Akmal, S.N. +6 more
core +1 more source
RAD50 missense variants differentially affect the DNA damage response and mitotic progression
FEBS Letters, EarlyView.RAD50 incorporates into the MRN complex and initiates the DNA damage response. Furthermore, RAD50 promotes mitotic progression. RAD50 missense variants capable of forming an MRN complex supported the DNA damage response and mitotic features to different extents in complementation experiments, indicating these functions are separable and might impact ...
Hanna Redeker +9 more
wiley +1 more source
Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]
, 2019 IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
Ackerman, A Lenore +8 more
core +3 more sources
Cell‐free DNA aneuploidy score as a dynamic early response marker in prostate cancer
Molecular Oncology, EarlyView.mFast‐SeqS‐based genome‐wide aneuploidy scores are concordant with aneuploidy scores obtained by whole genome sequencing from tumor tissue and can predict response to ARSI treatment at baseline and, at an early time point, to ARSI and taxanes. This assay can be easily performed at low cost and requires little input of cfDNA. Cell‐free circulating tumor
Khrystany T. Isebia +17 more
wiley +1 more source