Results 141 to 150 of about 649,005 (267)

A novel ψ-χ fusion protein for unravelling the contributions of χ to DNA replication and repair. [PDF]

Nucleic Acids Res
Padgett-Pagliai KA, Wimer E, Grant JD, Petrides MJ, Newcomb ESP, Sutera VA, Lovett ST, Bloom LB.   +7 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Continuously tunable multistability in DNA replication networks. [PDF]

Nat Commun
Zhong R, Fu Y, Jiang S, Wang S, Yue L, Tan W.   +5 more
europepmc   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

PAF15-PCNA exhaustion governs the strand-specific control of DNA replication. [PDF]

Nature
Chhetri G, Badugu SB, Petriman NA, Petersen MB, Güller AS, Fajri N, Coulée M, Pitchai GP, Novotný J, Larsen FT, Møller AF, Ebbesen MF, Ravnsborg T, Yadav AK, Balarasa B, Lunding A, Polasek-Sedlackova H, Jensen ON, Ravnskjaer K, Brewer JR, Madsen JGS, Petryk N, Andersen JS, Somyajit K.   +23 more
europepmc   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

TOP1 and TOP2 complementarily maintain DNA replication fork progression in vertebrates. [PDF]

J Biol Chem
Umemura K, Ooka M, Sojo M, Seki M, Xia M, Hirota K, Abe T.   +6 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Minute amounts of helicase-deficient truncated RECQL4 are sufficient for DNA replication. [PDF]

EMBO Rep
Buco PAV, Castillo-Tandazo W, Chalk AM, Pilcher C, Holien JK, Heierhorst J, Tan TY, Koren A, Smeets MF, Walkley CR.   +9 more
europepmc   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source