Results 11 to 20 of about 566,456 (323)
Nature Communications, 2022 Reusswig et al. use engineered systems to force DNA replication in the G1 phase of the cell cycle. This unscheduled G1 replication shows hallmarks of S phase replication, but leads to over-replication and DNA breaks from replication collisions.
Karl-Uwe Reusswig +7 more
doaj +1 more source
Chromatin and DNA Replication [PDF]
Cold Spring Harbor Perspectives in Biology, 2013 The size of a eukaryotic genome presents a unique challenge to the cell: package and organize the DNA to fit within the confines of the nucleus while at the same time ensuring sufficient dynamics to allow access to specific sequences and features such as genes and regulatory elements.
D. M. MacAlpine, G. Almouzni
openaire +3 more sources
Molecular choreography of primer synthesis by the eukaryotic Pol α-primase
Nature Communications, 2023 The eukaryotic polymerase α (Pol α) synthesizes an RNA-DNA hybrid primer of 20–30 nucleotides. Pol α is composed of Pol1, Pol12, Primase 1 (Pri1), and Pri2. Pol1 and Pri1 contain the DNA polymerase and RNA primase activities, respectively.
Zuanning Yuan +3 more
doaj +1 more source
eLife, 2015 We have reconstituted a eukaryotic leading/lagging strand replisome comprising 31 distinct polypeptides. This study identifies a process unprecedented in bacterial replisomes.
Roxana E Georgescu +7 more
doaj +1 more source
PLOS Genetics, 2019 In all kingdoms of life, DNA is used to encode hereditary information. Propagation of the genetic material between generations requires timely and accurate duplication of DNA by semiconservative replication prior to cell division to ensure each daughter cell receives the full complement of chromosomes.
Babatunde Ekundayo, Franziska Bleichert
openaire +5 more sources
Cell Reports, 2016 Temporal separation of DNA replication initiation into licensing and firing phases ensures the precise duplication of the genome during each cell cycle.
Karl-Uwe Reusswig +3 more
doaj +1 more source
Getting ready for DNA duplication
eLife, 2019 The discovery of a biomolecular condensate involved in DNA replication has wide-ranging implications.
Nina Y Yao, Michael E O'Donnell
doaj +1 more source
Cold Spring Harbor Perspectives in Biology, 2013 The onset of genomic DNA synthesis requires precise interactions of specialized initiator proteins with DNA at sites where the replication machinery can be loaded. These sites, defined as replication origins, are found at a few unique locations in all of the prokaryotic chromosomes examined so far.
A. C. Leonard, M. Mechali
openaire +3 more sources
Nucleosome-directed replication origin licensing independent of a consensus DNA sequence
Nature Communications, 2022 Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions
Sai Li +5 more
doaj +1 more source
DNA Replication Fidelity [PDF]
Journal of Biological Chemistry, 1992 ▪ Abstract DNA replication fidelity is a key determinant of genome stability and is central to the evolution of species and to the origins of human diseases. Here we review our current understanding of replication fidelity, with emphasis on structural and biochemical studies of DNA polymerases that provide new insights into the importance of hydrogen ...
openaire +3 more sources