Results 11 to 20 of about 3,431,424 (346)

Structures of 9-1-1 DNA checkpoint clamp loading at gaps from start to finish and ramification on biology

Cell Reports, 2023
Summary: Rad24-RFC (replication factor C) loads the 9-1-1 checkpoint clamp onto the recessed 5′ ends by binding a 5′ DNA at an external surface site and threading the 3′ single-stranded DNA (ssDNA) into 9-1-1. We find here that Rad24-RFC loads 9-1-1 onto
Fengwei Zheng, Roxana E. Georgescu, Nina Y. Yao, Michael E. O’Donnell, Huilin Li   +4 more
doaj   +1 more source

Chromatin and DNA Replication [PDF]

Cold Spring Harbor Perspectives in Biology, 2013
The size of a eukaryotic genome presents a unique challenge to the cell: package and organize the DNA to fit within the confines of the nucleus while at the same time ensuring sufficient dynamics to allow access to specific sequences and features such as genes and regulatory elements.
D. M. MacAlpine, G. Almouzni
openaire   +3 more sources

An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases

eLife, 2020
Cell cycle tags allow to restrict target protein expression to specific cell cycle phases. Here, we present an advanced toolbox of cell cycle tag constructs in budding yeast with defined and compatible peak expression that allow comparison of protein ...
Julia Bittmann, Rokas Grigaitis, Lorenzo Galanti, Silas Amarell, Florian Wilfling, Joao Matos, Boris Pfander   +6 more
doaj   +1 more source

Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions

Nature Communications, 2022
Reusswig et al. use engineered systems to force DNA replication in the G1 phase of the cell cycle. This unscheduled G1 replication shows hallmarks of S phase replication, but leads to over-replication and DNA breaks from replication collisions.
Karl-Uwe Reusswig, Julia Bittmann, Martina Peritore, Mathilde Courtes, Benjamin Pardo, Michael Wierer, Matthias Mann, Boris Pfander   +7 more
doaj   +1 more source

Molecular choreography of primer synthesis by the eukaryotic Pol α-primase

Nature Communications, 2023
The eukaryotic polymerase α (Pol α) synthesizes an RNA-DNA hybrid primer of 20–30 nucleotides. Pol α is composed of Pol1, Pol12, Primase 1 (Pri1), and Pri2. Pol1 and Pri1 contain the DNA polymerase and RNA primase activities, respectively.
Zuanning Yuan, Roxana Georgescu, Huilin Li, Michael E. O’Donnell   +3 more
doaj   +1 more source

Reconstitution of a eukaryotic replisome reveals suppression mechanisms that define leading/lagging strand operation

eLife, 2015
We have reconstituted a eukaryotic leading/lagging strand replisome comprising 31 distinct polypeptides. This study identifies a process unprecedented in bacterial replisomes.
Roxana E Georgescu, Grant D Schauer, Nina Y Yao, Lance D Langston, Olga Yurieva, Dan Zhang, Jeff Finkelstein, Mike E O'Donnell   +7 more
doaj   +1 more source

Implementing fluorescence enhancement, quenching, and FRET for investigating flap endonuclease 1 enzymatic reaction at the single-molecule level

Computational and Structural Biotechnology Journal, 2021
Flap endonuclease 1 (FEN1) is an important component of the intricate molecular machinery for DNA replication and repair. FEN1 is a structure-specific 5′ nuclease that cleaves nascent single-stranded 5′ flaps during the maturation of Okazaki fragments ...
Mohamed A. Sobhy, Muhammad Tehseen, Masateru Takahashi, Amer Bralić, Alfredo De Biasio, Samir M. Hamdan   +5 more
doaj   +1 more source

Robust Replication Control Is Generated by Temporal Gaps between Licensing and Firing Phases and Depends on Degradation of Firing Factor Sld2

Cell Reports, 2016
Temporal separation of DNA replication initiation into licensing and firing phases ensures the precise duplication of the genome during each cell cycle.
Karl-Uwe Reusswig, Fabian Zimmermann, Lorenzo Galanti, Boris Pfander   +3 more
doaj   +1 more source

Proteomic data on the nuclear interactome of human MCM9

Data in Brief, 2016
We present data relating to the interactome of MCM9 from the nuclei of human cells. MCM9 belongs to the AAA+ superfamily, and contains an MCM domain and motifs that may confer DNA helicase activity.
James R.A. Hutchins, Sabine Traver, Philippe Coulombe, Isabelle Peiffer, Magali Kitzmann, Daniel Latreille, Marcel Méchali   +6 more
doaj   +1 more source

Origins of DNA replication

PLOS Genetics, 2019
In all kingdoms of life, DNA is used to encode hereditary information. Propagation of the genetic material between generations requires timely and accurate duplication of DNA by semiconservative replication prior to cell division to ensure each daughter cell receives the full complement of chromosomes.
Babatunde Ekundayo, Franziska Bleichert
openaire   +5 more sources