Results 11 to 20 of about 563,532 (298)
The Replication of Polyoma DNA
Journal of General Virology, 1973 Summary Measurement of replicating molecules of polyoma virus DNA after digestion with endonuclease R1 shows that DNA replication is bidirectional, starting predominantly at a specific site. In both large plaque and small plaque polyoma virus DNA this site is 29 ± 2% of the total length of the DNA from the cleavage site of the endonuclease R1.
L. V. Crawford +2 more
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MTBP phosphorylation controls DNA replication origin firing
Scientific Reports, 2021 Faithful genome duplication requires regulation of origin firing to determine loci, timing and efficiency of replisome generation. Established kinase targets for eukaryotic origin firing regulation are the Mcm2-7 helicase, Sld3/Treslin/TICRR and Sld2 ...
Pedro Ferreira +12 more
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Nature Communications, 2022 Here the authors describe multiple structures of the replicative helicase MCM2-7 in complex with Dbf4-dependent kinase (DDK). These structures reveal why the kinase specifically recognizes the MCM2-7 double-hexamer over the single-hexamer and explain how
Almutasem Saleh +9 more
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The Contribution of Lysosomes to DNA Replication
Cells, 2021 Lysosomes, acidic, membrane-bound organelles, are not only the core of the cellular recycling machinery, but they also serve as signaling hubs regulating various metabolic pathways. Lysosomes maintain energy homeostasis and provide pivotal substrates for
Joanna Maria Merchut-Maya +1 more
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eLife, 2022 RFC uses ATP to assemble PCNA onto primed sites for replicative DNA polymerases δ and ε. The RFC pentamer forms a central chamber that binds 3′ ss/ds DNA junctions to load PCNA onto DNA during replication.
Fengwei Zheng +4 more
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Adenovirus DNA Replication [PDF]
Cold Spring Harbor Perspectives in Biology, 2013 Adenoviruses have attracted much attention as probes to study biological processes such as DNA replication, transcription, splicing, and cellular transformation. More recently these viruses have been used as gene-transfer vectors and oncolytic agents.
Hoeben, R.C., Uil, T.G.
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Nature Communications, 2021 Eukaryotic DNA replication is mediated by many proteins which are tightly regulated for an efficient firing of replication at each cell cycle. Here the authors report a cryo-EM structure of the yeast ORC–Cdc6 bound to an 85-bp ARS1 origin DNA revealing ...
Xiang Feng +5 more
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Nature Communications, 2022 Reusswig et al. use engineered systems to force DNA replication in the G1 phase of the cell cycle. This unscheduled G1 replication shows hallmarks of S phase replication, but leads to over-replication and DNA breaks from replication collisions.
Karl-Uwe Reusswig +7 more
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eLife, 2020 Cell cycle tags allow to restrict target protein expression to specific cell cycle phases. Here, we present an advanced toolbox of cell cycle tag constructs in budding yeast with defined and compatible peak expression that allow comparison of protein ...
Julia Bittmann +6 more
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Chromatin and DNA Replication [PDF]
Cold Spring Harbor Perspectives in Biology, 2013 The size of a eukaryotic genome presents a unique challenge to the cell: package and organize the DNA to fit within the confines of the nucleus while at the same time ensuring sufficient dynamics to allow access to specific sequences and features such as genes and regulatory elements.
D. M. MacAlpine, G. Almouzni
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