Results 21 to 30 of about 649,005 (267)
eLife, 2015 We have reconstituted a eukaryotic leading/lagging strand replisome comprising 31 distinct polypeptides. This study identifies a process unprecedented in bacterial replisomes.
Roxana E Georgescu +7 more
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Molecular choreography of primer synthesis by the eukaryotic Pol α-primase
Nature Communications, 2023 The eukaryotic polymerase α (Pol α) synthesizes an RNA-DNA hybrid primer of 20–30 nucleotides. Pol α is composed of Pol1, Pol12, Primase 1 (Pri1), and Pri2. Pol1 and Pri1 contain the DNA polymerase and RNA primase activities, respectively.
Zuanning Yuan +3 more
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Cell Reports, 2016 Temporal separation of DNA replication initiation into licensing and firing phases ensures the precise duplication of the genome during each cell cycle.
Karl-Uwe Reusswig +3 more
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Computational and Structural Biotechnology Journal, 2021 Flap endonuclease 1 (FEN1) is an important component of the intricate molecular machinery for DNA replication and repair. FEN1 is a structure-specific 5′ nuclease that cleaves nascent single-stranded 5′ flaps during the maturation of Okazaki fragments ...
Mohamed A. Sobhy +5 more
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Proteomic data on the nuclear interactome of human MCM9
Data in Brief, 2016 We present data relating to the interactome of MCM9 from the nuclei of human cells. MCM9 belongs to the AAA+ superfamily, and contains an MCM domain and motifs that may confer DNA helicase activity.
James R.A. Hutchins +6 more
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Getting ready for DNA duplication
eLife, 2019 The discovery of a biomolecular condensate involved in DNA replication has wide-ranging implications.
Nina Y Yao, Michael E O'Donnell
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Nucleosome-directed replication origin licensing independent of a consensus DNA sequence
Nature Communications, 2022 Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions
Sai Li +5 more
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Quantitative sensing and signalling of single-stranded DNA during the DNA damage response
Nature Communications, 2019 DNA damage triggers checkpoint signalling mechanisms. Here the authors reveal differential phosphorylation of targets of the Mec1-Ddc2 checkpoint kinase by analyzing the effect of quantitatively different ssDNA signals.
Susanne C. S. Bantele +2 more
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SARS-CoV-2: from its discovery to genome structure, transcription, and replication
Cell & Bioscience, 2021 SARS-CoV-2 is an extremely contagious respiratory virus causing adult atypical pneumonia COVID-19 with severe acute respiratory syndrome (SARS). SARS-CoV-2 has a single-stranded, positive-sense RNA (+RNA) genome of ~ 29.9 kb and exhibits significant ...
Ayslan Castro Brant +4 more
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Molecular Docking for the Development of Alternative Therapies against Leishmaniasis
Chemistry Proceedings, 2023 Topoisomerases play a pivotal role in regulating the topological structure of DNA during fundamental processes such as transcription, DNA repair, or DNA replication; because of this, topoisomerases are biological targets in pathogenic microorganisms or ...
Juan Diego Guarimata +3 more
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