Results 31 to 40 of about 910,557 (358)

The dynamics of replication licensing in live Caenorhabditis elegans embryos [PDF]

, 2012
Accurate DNA replication requires proper regulation of replication licensing, which entails loading MCM-2-7 onto replication origins. In this paper, we provide the first comprehensive view of replication licensing in vivo, using video microscopy of ...
Anton Gartner, Ashley Craig, Baldinger, Blow, Blow, Brauchle, Brenner, Cheeseman, Chen, Clarey, Clarey, DePamphilis, Drury, Edgar, Encalada, Evrin, Galy, Gambus, Gillespie, Hadwiger, Harvey, Ilves, J. Julian Blow, Kamath, Kim, Kisielewska, Korzelius, Kuipers, Li, Li, Li, Liu, Matthieu Querenet, McNairn, McNally, Moyer, Oehlmann, Praitis, Prokhorova, Quinn, Randell, Remi Sonneville, Remus, Rothbauer, Rowles, Shelton, Siddiqui, Speck, Sönnichsen, Tada, Timmons, Waga, Wallenfang, Wohlschlegel, Yanagi, Yang, Zhong   +56 more
core   +3 more sources

Replicative DNA Polymerases [PDF]

Cold Spring Harbor Perspectives in Biology, 2013
In 1959, Arthur Kornberg was awarded the Nobel Prize for his work on the principles by which DNA is duplicated by DNA polymerases. Since then, it has been confirmed in all branches of life that replicative DNA polymerases require a single-stranded template to build a complementary strand, but they cannot start a new DNA strand de novo.
Johansson, Erik, Dixon, Nicholas
openaire   +4 more sources

Nucleosome-directed replication origin licensing independent of a consensus DNA sequence

Nature Communications, 2022
Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions
Sai Li, Michael R. Wasserman, Olga Yurieva, Lu Bai, Michael E. O’Donnell, Shixin Liu   +5 more
doaj   +1 more source

DNA Replication Fidelity [PDF]

Journal of Biological Chemistry, 1992
▪ Abstract DNA replication fidelity is a key determinant of genome stability and is central to the evolution of species and to the origins of human diseases. Here we review our current understanding of replication fidelity, with emphasis on structural and biochemical studies of DNA polymerases that provide new insights into the importance of hydrogen ...
openaire   +3 more sources

Persistence length of chromatin determines origin spacing in Xenopus early-embryo DNA replication: Quantitative comparisons between theory and experiment [PDF]

Cell Cycle 3, 223-229 (2004), 2003
In Xenopus early embryos, replication origins neither require specific DNA sequences nor is there an efficient S/M checkpoint, even though the whole genome (3 billion bases) is completely duplicated within 10-20 minutes. This leads to the"random-completion problem" of DNA replication in embryos, where one needs to find a mechanism that ensures complete,
arxiv   +1 more source

Impaired DNA replication within progenitor cell pools promotes leukemogenesis. [PDF]

, 2005
Impaired cell cycle progression can be paradoxically associated with increased rates of malignancies. Using retroviral transduction of bone marrow progenitors followed by transplantation into mice, we demonstrate that inhibition of hematopoietic ...
Ames, Anderson, Andriy Marusyk, Begg, Blagosklonny, Calabretta, Castor, Chang, Choi, Christensen, Christopher C Porter, Christopher Kemp, DeGregori, Dimova, Field, Furgerson, Ganna Bilousova, Gottlieb, Greaves, Hanahan, Hariharan, Jacks, James DeGregori, Jamieson, Just, Kinder, Li, Li, Lightfoot, Lin, Luch, Lundin, Matioli, Milner, Murga, Passegue, Pear, Penit, Pui, Robert D Cardiff, Ryan, Saintigny, Sawyers, Schaefer, Shechter, Sirard, Skibola, Skorski, Spangrude, Sterkers, Van Etten, Wallace-Brodeur, Wong, Wu, Yamasaki, Zhang, Zhu, Zimmerman   +57 more
core   +3 more sources

Quantitative sensing and signalling of single-stranded DNA during the DNA damage response

Nature Communications, 2019
DNA damage triggers checkpoint signalling mechanisms. Here the authors reveal differential phosphorylation of targets of the Mec1-Ddc2 checkpoint kinase by analyzing the effect of quantitatively different ssDNA signals.
Susanne C. S. Bantele, Michael Lisby, Boris Pfander   +2 more
doaj   +1 more source

Nucleation and growth in one dimension, part II: Application to DNA replication kinetics [PDF]

Phys. Rev. E 71, 011909 (2005), 2004
Inspired by recent experiments on DNA replication, we apply a one-dimensional nucleation-and-growth model to DNA-replication kinetics, focusing on how to extract the time-dependent nucleation rate I(t) and growth speed v from data. We discuss generic experimental problems, namely spatial inhomogeneity, measurement noise, and finite-size effects.
arxiv   +1 more source

Error correction during DNA replication [PDF]

Physical Review E, VOL.86, 011913 (2012), 2011
DNA polymerase (DNAP) is a dual-purpose enzyme that plays two opposite roles in two different situations during DNA replication. It plays its normal role as a {\it polymerase} catalyzing the elongation of a new DNA molecule by adding a monomer. However, it can switch to the role of an {\it exonuclease} and shorten the same DNA by cleavage of the last ...
arxiv   +1 more source

Genetic and physical mapping of DNA replication origins in Haloferax volcanii [PDF]

, 2007
The halophilic archaeon Haloferax volcanii has a multireplicon genome, consisting of a main chromosome, three secondary chromosomes, and a plasmid. Genes for the initiator protein Cdc6/Orc1, which are commonly located adjacent to archaeal origins of DNA ...
Allers, T, Eisen, JA, Hartman, AL, Hawkins, M, Myllykallio, H, Norais, C   +5 more
core   +6 more sources