Quantitative sensing and signalling of single-stranded DNA during the DNA damage response
Nature Communications, 2019 DNA damage triggers checkpoint signalling mechanisms. Here the authors reveal differential phosphorylation of targets of the Mec1-Ddc2 checkpoint kinase by analyzing the effect of quantitatively different ssDNA signals.
Susanne C. S. Bantele +2 more
doaj +1 more source
Damage-induced phosphorylation of Sld3 is important to block late origin firing. [PDF]
, 2010 Origins of replication are activated throughout the S phase of the cell cycle such that some origins fire early and others fire late to ensure that each chromosome is completely replicated in a timely fashion.
Defazio-Eli, Lisa G +5 more
core +2 more sources
DNA Replication Fidelity [PDF]
Journal of Biological Chemistry, 1992 ▪ Abstract DNA replication fidelity is a key determinant of genome stability and is central to the evolution of species and to the origins of human diseases. Here we review our current understanding of replication fidelity, with emphasis on structural and biochemical studies of DNA polymerases that provide new insights into the importance of hydrogen ...
openaire +3 more sources
The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress [PDF]
, 2015 The MRE11/RAD50/NBS1 (MRN) complex is a major sensor of DNA double strand breaks, whose role in controlling faithful DNA replication and preventing replication stress is also emerging. Inactivation of the MRN complex invariably leads to developmental and/
A Dupre +70 more
core +1 more source
eLife, 2017 DNA double strand breaks (DSBs) can be repaired by either recombination-based or direct ligation-based mechanisms. Pathway choice is made at the level of DNA end resection, a nucleolytic processing step, which primes DSBs for repair by recombination ...
Susanne CS Bantele +4 more
doaj +1 more source
SARS-CoV-2: from its discovery to genome structure, transcription, and replication
Cell & Bioscience, 2021 SARS-CoV-2 is an extremely contagious respiratory virus causing adult atypical pneumonia COVID-19 with severe acute respiratory syndrome (SARS). SARS-CoV-2 has a single-stranded, positive-sense RNA (+RNA) genome of ~ 29.9 kb and exhibits significant ...
Ayslan Castro Brant +4 more
doaj +1 more source
Molecular Docking for the Development of Alternative Therapies against Leishmaniasis
Chemistry Proceedings, 2023 Topoisomerases play a pivotal role in regulating the topological structure of DNA during fundamental processes such as transcription, DNA repair, or DNA replication; because of this, topoisomerases are biological targets in pathogenic microorganisms or ...
Juan Diego Guarimata +3 more
doaj +1 more source
Evidence for a role of Arabidopsis CDT1 proteins in gametophyte development and maintenance of genome integrity [PDF]
, 2012 Meristems retain the ability to divide throughout the life cycle of plants, which can last for over 1000 years in some species. Furthermore, the germline is not laid down early during embryogenesis but originates from the meristematic cells relatively ...
Benhamed, Moussa +8 more
core +3 more sources
Attenuation of reverse transcriptase facilitates SAMHD1 restriction of HIV-1 in cycling cells
Retrovirology, 2023 Background SAMHD1 is a deoxynucleotide triphosphohydrolase that restricts replication of HIV-1 in differentiated leucocytes. HIV-1 is not restricted in cycling cells and it has been proposed that this is due to phosphorylation of SAMHD1 at T592 in these ...
Ming-Han C. Tsai +8 more
doaj +1 more source
PrimPol is required for replicative tolerance of G quadruplexes in vertebrate cells [PDF]
, 2016 G quadruplexes (G4s) can present potent blocks to DNA replication. Accurate and timely replication of G4s in vertebrates requires multiple specialized DNA helicases and polymerases to prevent genetic and epigenetic instability.
Bailey, Laura J +7 more
core +1 more source