Results 111 to 120 of about 673,449 (404)

Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase.

BioTechniques, 2001
Here, we describe a method that offers a unique way to engineer plasmids with precision but without digestion using restriction enzymes for the insertion of DNA.
M. Geiser, R. Cébe, Delia Drewello, R. Schmitz   +3 more
semanticscholar   +1 more source

The MmeI family: type II restriction–modification enzymes that employ single-strand modification for host protection [PDF]

, 2009
The type II restriction endonucleases form one of the largest families of biochemically-characterized proteins. These endonucleases typically share little sequence similarity, except among isoschizomers that recognize the same sequence.
Altschul, Barker, Bath, Bennett-Lovsey, Brown, Bujnicki, Cesnaviciene, Dunin-Horkawicz, Elizabeth A. Dwinell, Elizabeth M. Lang, Embleton, Janulaitis, Janulaitis, Kelleher, Kim, Kinch, Kong, Kong, Malone, Marchler-Bauer, Morgan, Nakonieczna, Niv, Pawlak, Pei, Perler, Posfai, Raleigh, Richard D. Morgan, Roberts, Roberts, Roberts, Samuelson, Schildkraut, Shinomiya, Skoglund, Skowron, Tanya K. Bhatia, Vanamee, Xu, Yvette A. Luyten   +40 more
core   +2 more sources

Combined spatially resolved metabolomics and spatial transcriptomics reveal the mechanism of RACK1‐mediated fatty acid synthesis

Molecular Oncology, EarlyView.
The authors analyzed the spatial distributions of gene and metabolite profiles in cervical cancer through spatial transcriptomic and spatially resolved metabolomic techniques. Pivotal genes and metabolites within these cases were then identified and validated.
Lixiu Xu, Jinqiu Li, Junqi Ma, Ayshamgul Hasim   +3 more
wiley   +1 more source

Restriction and modification of deoxyarchaeosine (dG+)-containing phage 9 g DNA

Scientific Reports, 2017
E. coli phage 9 g contains the modified base deoxyarchaeosine (dG+) in its genome. The phage encodes its own primase, DNA ligase, DNA polymerase, and enzymes necessary to synthesize and incorporate dG+.
Rebecca Tsai, Ivan R. Corrêa, Michael Y. Xu, Shuang-yong Xu   +3 more
doaj   +1 more source

Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5' overhangs with run through stop DNA synthesis utilizing Ara-C

BMC Biotechnology, 2005
Background Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize ...
Silverman Mel, Goldenberg Neil M, Ailenberg Menachem   +2 more
doaj   +1 more source

A prediction of the amino acids and structures involved in DNA recognition by type I DNA restriction and modification enzymes.

Nucleic Acids Research, 1997
The S subunits of type I DNA restriction/modification enzymes are responsible for recognising the DNA target sequence for the enzyme. They contain two domains of approximately 150 amino acids, each of which is responsible for recognising one half of the ...
S. Sturrock, D. Dryden
semanticscholar   +1 more source

Characterization of the DNA of Canine Adenovirus by Restriction Enzyme Analysis

Intervirology, 1985
The DNA of canine adenovirus (canAV 1, strain Behring H.c.c. 269) was characterized in detail. The molecular weight of canAV DNA was (20.1 +/- 0.1) x 10(6) daltons as determined by contour length measurements, and 19.9 x 10(6) daltons as determined by restriction enzyme analysis.
Angela Rösen, Gholamreza Darai, Rolf M. Flügel, Hajo Delius   +3 more
openaire   +3 more sources

Replenishing co‐downregulated miR‐100‐5p and miR‐125b‐5p in malignant germ cell tumors causes growth inhibition through cell cycle disruption

Molecular Oncology, EarlyView.
MiR‐99a‐5p/miR‐100‐5p (functionally identical) and miR‐125b‐5p microRNAs are downregulated in malignant germ cell tumors (GCTs). Combination replenishment of these microRNAs using mimics resulted in growth inhibition in representative cell lines, with consequent downregulation of target genes involved in cell cycle (confirmed by flow cytometry) and ...
Marta Ferraresso, Shivani Bailey, Luz Alonso‐Crisostomo, Dawn Ward, Christos Panayi, Zachary G. L. Scurlock, Harpreet K. Saini, Stephen P. Smith, James C. Nicholson, Anton J. Enright, Cinzia G. Scarpini, Nicholas Coleman, Matthew J. Murray   +12 more
wiley   +1 more source

Investigation of DNA-protein Sequence-Specific Interactions with a ds-DNA Array

Molecules, 2005
The sequence specific recognitions between DNAs and proteins play important roles in many biological functions. The use of double-stranded DNA arrays (ds-DNA arrays) for studying sequence specific recognition between DNAs and proteins is a promising ...
Zuhong Lu, Quanjun Liu, Tongxiang Li, Jinke Wang, Qinyu Ge, Yunfei Bai   +5 more
doaj   +1 more source

Cleavage patterns of Drosophila melanogaster satellite DNA by restriction enzymes.

Nucleic Acids Research, 1976
The five satellite DNAs of Drosophila melanogaster have been isolated by the combined use of different equilibrium density gradients and hydrolyzed by seven different restriction enzymes; Hae III, Hind II + Hind III, Hinf, Hpa II, EcoR I and EcoR II. The
C. Shen, G. Wiesehahn, J. Hearst
semanticscholar   +1 more source