Results 121 to 130 of about 397,302 (354)
The role of DNA restriction-modification systems in the biology of Bacillus anthracis
Frontiers in Microbiology, 2016 Restriction-modification (R-M) systems are widespread among prokaryotes and, depending on their type, may be viewed as selfish genetic elements that persist as toxin-antitoxin modules or as cellular defense systems against phage infection.
Ramakrishnan eSitaraman
doaj +1 more source
, 2010 We propose a novel theoretical biomolecular design to implement any Boolean circuit using the mechanism of DNA strand displacement. The design is scalable: all species of DNA strands can in principle be mixed and prepared in a single test tube, rather ...
A. Goel +8 more
core +2 more sources
Isolation of large molecular weight DNA from agarose gels forfurther digestion by restriction enzymes [PDF]
, 1975 Nikolaus Blin +2 more
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Molecular Oncology, EarlyView.This study investigates gene expression differences between two major pediatric acute lymphoblastic leukemia (ALL) subtypes, B‐cell precursor ALL, and T‐cell ALL, using a data‐driven approach consisting of biostatistics and machine learning methods. Following analysis of a discovery dataset, we find a set of 14 expression markers differentiating the ...
Mona Nourbakhsh +8 more
wiley +1 more source
Simple enzymatic means to neutralize DNA contamination in nucleic acid amplification
BioTechniques, 2005 Reverse transcription PCR (RT-PCR) is prone to false positives when contaminating DNA molecules are present at the start of a reaction. Contaminants that derive from earlier work using a given primer pair (carryover PCR products) are of particular ...
John Ashkenas +2 more
doaj +1 more source
Restoration by T4 ligase of DNA sequences sensitive to “flush”-cleaving restriction enzyme [PDF]
, 1977 Monica Mottes +3 more
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Molecular Oncology, EarlyView.mir‐196a promotes Esophagus Adenocarcinoma aggressiveness. On one hand, mir‐196a targets the valosin‐containing protein (VCP) mRNA, causing the accumulation of c‐MYC protein that leads to high amounts of TERT. On the other hand, mir‐196a targets the inhibitor of NFκB (NFKBIA).
Jesús García‐Castillo +8 more
wiley +1 more source
Nature Communications, 2019 Genomics DNA library preparation from formalin-fixed paraffin-embedded tissues is challenging. Here the authors describe CUTseq that uses restriction enzymes and in vitro amplification to barcode samples for reduced representation genome sequencing.
Xiaolu Zhang +11 more
doaj +1 more source
Sites of cleavage by restriction enzymes in viral DNAs: comparison of observed and expected frequencies [PDF]
, 1974 R. A. Elton
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Molecular Oncology, EarlyView.The pan‐HDAC inhibitor belinostat increases the expression of the pro‐apoptotic proteins Bim, Puma, and Noxa and induces apoptosis in ovarian cancer cell lines and patient‐derived tumor organoids when used at high concentrations. Moreover, inhibiting the anti‐apoptotic proteins Bcl‐xL or Mcl‐1 sensitizes these preclinical models to the cytotoxic effect
Cécilia Thomine +10 more
wiley +1 more source