Results 131 to 140 of about 634,910 (347)

A Small Family of Elements with Long Inverted Repeats is Located Near Sites of Developmentally Regulated DNA Rearrangement in \u3cem\u3eTetrahymena thermophila\u3c/em\u3e [PDF]

, 1994
Extensive DNA rearrangement occurs during the development of the somatic macronucleus from the germ line micronucleus in ciliated protozoans. The micronuclear junctions and the macronuclear product of a developmentally regulated DNA rearrangement in ...
Berger, Patricia J., Catt, Diana M., Ellingson, Jay Lee Edward, Karrer, Kathleen M., Wells, John M.   +4 more
core   +1 more source

Glycosylated LGALS3BP is highly secreted by bladder cancer cells and represents a novel urinary disease biomarker

Molecular Oncology, EarlyView.
Urinary LGALS3BP is elevated in bladder cancer patients compared to healthy controls as detected by the 1959 antibody–based ELISA. The antibody shows enhanced reactivity to the high‐mannose glycosylated variant secreted by cancer cells treated with kifunensine (KIF).
Asia Pece, Giulio Lovato, Ilaria Cela, Arianna Mercatelli, Benedetta Ferro, Jussi Nikkola, Sara Pagotto, Tommaso Grottola, Vincenzo De Laurenzi, Rossella Cicchetti, Antonio Marchetti, Luigi Schips, Rossano Lattanzio, Stefano Iacobelli, Emily Capone, Peter Black, Mads Daugaard, Michele Marchioni, Gianluca Sala   +18 more
wiley   +1 more source

Simple enzymatic means to neutralize DNA contamination in nucleic acid amplification

BioTechniques, 2005
Reverse transcription PCR (RT-PCR) is prone to false positives when contaminating DNA molecules are present at the start of a reaction. Contaminants that derive from earlier work using a given primer pair (carryover PCR products) are of particular ...
John Ashkenas, James W. Dennis, Chi Yip Ho   +2 more
doaj   +1 more source

Survivin and Aurora Kinase A control cell fate decisions during mitosis

Molecular Oncology, EarlyView.
Aurora A interacts with survivin during mitosis and regulates its centromeric role. Loss of Aurora A activity mislocalises survivin, the CPC and BubR1, leading to disruption of the spindle checkpoint and triggering premature mitotic exit, which we refer to as ‘mitotic slippage’.
Hana Abdelkabir, Shalitha Wickrama Arachchige, Sally P. Wheatley   +2 more
wiley   +1 more source

CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples

Nature Communications, 2019
Genomics DNA library preparation from formalin-fixed paraffin-embedded tissues is challenging. Here the authors describe CUTseq that uses restriction enzymes and in vitro amplification to barcode samples for reduced representation genome sequencing.
Xiaolu Zhang, Silvano Garnerone, Michele Simonetti, Luuk Harbers, Marcin Nicoś, Reza Mirzazadeh, Tiziana Venesio, Anna Sapino, Johan Hartman, Caterina Marchiò, Magda Bienko, Nicola Crosetto   +11 more
doaj   +1 more source

A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes [PDF]

, 2017
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different Type II enzymes now identified and provides a system for ...
Belfort, Marlene, Bestor, Timothy, Bhagwat, Ashok S., Bickle, Thomas A., Bitinaite, Jurate, Blumenthal, Robert M., Degtyarev, Sergey Kh, Dryden, David T. F., Dybvig, Kevin, Firman, Keith, Gromova, Elizaveta S., Gumport, Richard I., Halford, Stephen E., Hattman, Stanley, Heitman, Joseph, Hornby, David P., Janulaitis, Arvydas, Jeltsch, Albert, Josephsen, Jytte, Kiss, Antal, Klaenhammer, Todd R., Kobayashi, Ichizo, Kong, Huimin, Krüger, Detlev H., Lacks, Sanford, Marinus, Martin G., Miyahara, Michiko, Morgan, Richard D., Murray, Noreen E., Nagaraja, Valakunja, Piekarowicz, Andrzej, Pingoud, Alfred, Raleigh, Elisabeth, Rao, Desirazu N., Reich, Norbert, Repin, Vladimir E., Roberts, Richard J., Selker, Eric U., Shaw, Pang‐Chui, Stein, Daniel C., Stoddard, Barry L., Szybalski, Waclaw, Trautner, Thomas A., Van Etten, James L., Vitor, Jorge M. B., Wilson, Geoffrey G., Xu, Shuang‐yong   +46 more
core  

Scalable, Time-Responsive, Digital, Energy-Efficient Molecular Circuits using DNA Strand Displacement

, 2010
We propose a novel theoretical biomolecular design to implement any Boolean circuit using the mechanism of DNA strand displacement. The design is scalable: all species of DNA strands can in principle be mixed and prepared in a single test tube, rather ...
A. Goel, B. Yurke, D.Y. Zhang, G. Seelig, J. Macdonald, L. Qian, M.N. Stojanovic, M.O. Magnasco, R. Penchovsky   +8 more
core   +2 more sources

Liquid biopsy epigenetics: establishing a molecular profile based on cell‐free DNA

Molecular Oncology, EarlyView.
Cell‐free DNA (cfDNA) fragments in plasma from cancer patients carry epigenetic signatures reflecting their cells of origin. These epigenetic features include DNA methylation, nucleosome modifications, and variations in fragmentation. This review describes the biological properties of each feature and explores optimal strategies for harnessing cfDNA ...
Christoffer Trier Maansson, Anders Lade Nielsen, Boe Sandahl Sorensen   +2 more
wiley   +1 more source

Activity on non-methylated DNA limits the use of endonuclease MspJI for epigenetic analyses

All Results Journals: Biol, 2018
Cytosine methylation of DNA in mammals has been associated with both physiological and pathological changes in gene-expression. DNA treatment with methylation sensitive and/or dependent restriction enzymes, followed by PCR amplification is a widely used ...
María Belén Jerez, Maximiliano Juri Ayub   +1 more
doaj  

Selective microbial genomic DNA isolation using restriction endonucleases.

PLoS ONE, 2014
To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads.
Helen E Barnes, Guohong Liu, Christopher Q Weston, Paula King, Long K Pham, Shannon Waltz, Kimberly T Helzer, Laura Day, Dan Sphar, Robert T Yamamoto, R Allyn Forsyth   +10 more
doaj   +1 more source