Results 131 to 140 of about 465,116 (357)
Application of Oligonucleotide Activation to Restriction Endonuclease NarI
BioTechniques, 1997 Restriction endonuclease NarI cleaves DNA using a two-site mechanism, placing it in the Type IIe class of restriction endonucleases. Although these enzymes have very useful recognition sequences, the two-site mechanism limits the practical application ...
Joseph H. Senesac, Jennifer K. Romanin
doaj +1 more source
Quantitative approach to facilitated diffusion with intersegmental jumping [PDF]
Proc. Natl. Acad. Sci. USA 106, 8204 (2009), 2008 We study the impact of DNA coiling on the search rate of proteins moving along the DNA contour interspersed by three-dimensional (3D) bulk excursions. When the DNA is coiled proteins performing short 3D hops along a DNA segment can be captured by foreign DNA segments that have looped back close to the original segment.
arxiv
Molecular Oncology, EarlyView.Analysis of ESR1 mutations in plasma cell‐free DNA (cfDNA) is highly important for the selection of treatment in patients with breast cancer. Using multiplex‐ddPCR and identical blood draws, we investigated whether circulating tumor cells (CTCs) and cfDNA provide similar or complementary information for ESR1 mutations.
Stavroula Smilkou +11 more
wiley +1 more source
Sliding and jumping of single EcoRV restriction enzymes on non-cognate DNA
Nucleic Acids Research, 2008 The restriction endonuclease EcoRV can rapidly locate a short recognition site within long non-cognate DNA using ‘facilitated diffusion’. This process has long been attributed to a sliding mechanism, in which the enzyme first binds to the DNA via ...
I. Bonnet +9 more
semanticscholar +1 more source
Water-mediated correlations in DNA-enzyme interactions [PDF]
arXiv, 2016 In this paper we consider dipole-mediated correlations between DNA and enzymes in the context of their water environment. Such correlations emerge from electric dipole-dipole interactions between aromatic ring structures in DNA and in enzymes. We show that there are matching collective modes between DNA and enzyme dipole fields, and that a dynamic time-
arxiv
Novel non-specific DNA adenine methyltransferases [PDF]
, 2011 The mom gene of bacteriophage Mu encodes an enzyme that converts adenine to N6-(1-acetamido)-adenine in the phage DNA and thereby protects the viral genome from cleavage by a wide variety of restriction endonucleases.
Altschul +46 more
core +2 more sources
Targeted metabolomics reveals novel diagnostic biomarkers for colorectal cancer
Molecular Oncology, EarlyView.This study employed targeted metabolomic profiling to identify 302 distinct metabolites present in platelet‐rich plasma (PRP), revealing aberrant metabolic profiles amongst individuals diagnosed with colorectal cancer (CRC). Compared to carcinoembryonic antigen (CEA) and cancer antigen 19‐9 (CA199), our metabolite panel showed improved sensitivity ...
Zuojian Hu +7 more
wiley +1 more source
Terminal Restriction Fragments (TRF) Method to Analyze Telomere Lengths
Bio-Protocol, 2015 Chromosome ends - telomeres - are a focus of intensive research due to their importance for the maintenance of chromosome stability. Their shortening due to incomplete replication functions as a molecular clock counting the number of cell divisions, and ...
Miloslava Fojtová +3 more
doaj +1 more source
Epidemiology and Infection, 1989 SUMMARY A series of clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) from two hospitals in China was examined. Fragment patterns obtained by digestion of total cellular DNA with restriction enzymes were used to characterize the ...
L. Hall, J. Jordens, F. Wang
semanticscholar +1 more source
MITOCHONDRIAL DNA POLYMORPHISMS AND FERTILITY IN BEEF CATTLE [PDF]
, 2002 Two regions of mitochondrial DNA, D-loop and ND-5 were characterized using polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) involving 422 beef cattle of Hereford and composite breeds from Wokalup’s ...
., Sutarno +3 more
core