Results 131 to 140 of about 397,302 (354)

Activity on non-methylated DNA limits the use of endonuclease MspJI for epigenetic analyses

All Results Journals: Biol, 2018
Cytosine methylation of DNA in mammals has been associated with both physiological and pathological changes in gene-expression. DNA treatment with methylation sensitive and/or dependent restriction enzymes, followed by PCR amplification is a widely used ...
María Belén Jerez, Maximiliano Juri Ayub   +1 more
doaj  

Selective microbial genomic DNA isolation using restriction endonucleases.

PLoS ONE, 2014
To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads.
Helen E Barnes, Guohong Liu, Christopher Q Weston, Paula King, Long K Pham, Shannon Waltz, Kimberly T Helzer, Laura Day, Dan Sphar, Robert T Yamamoto, R Allyn Forsyth   +10 more
doaj   +1 more source

Cleavage of Simian Virus 40 DNA at a Unique Site by a Bacterial Restriction Enzyme [PDF]

, 1972
John F. Morrow, Paul Berg
openalex   +1 more source

Molecular imaging predicts trastuzumab‐deruxtecan (T‐DXd) response in head and neck cancer xenograft models

Molecular Oncology, EarlyView.
Trastuzumab‐deruxtecan, a HER2‐targeting antibody‐drug conjugate, shows promising antitumor activity in head and neck squamous cell carcinoma with low HER2 expression. In vitro and in vivo studies demonstrated dose‐dependent cell death and tumor growth reduction in low HER2‐expressing cell lines, which correlated with drug accumulation measured using a
Abdullah Bin Naveed, Lucas Mani, Muhammad Bilal Mirza, Ashtyn McAdoo, Takahito Kondo, Hidenori Tanaka, Nicole Meeks, Eben Rosenthal, Marisa Hom   +8 more
wiley   +1 more source

A key to selected rockfishes (Sebastes spp.) based on mitochondrial DNA restriction fragment analysis [PDF]

, 2006
Larval and juvenile rockfishes (Sebastes spp.) are difficult to identify using morphological characters. We developed a key based on sizes of restriction endonuclease fragments of the NADH dehydrogenase-3 and -4 (ND3/ND4) and 12S and 16S ribosomal RNA ...
Asahida, Takashi, Gharrett, Anthony J., Goto, Akira, Gray, Andrew K., Li, Zhuozhuo, Love, Milton S.   +5 more
core  

A nucleotide‐independent, pan‐RAS‐targeted DARPin elicits anti‐tumor activity in a multimodal manner

Molecular Oncology, EarlyView.
We report a Designed Ankyrin Repeat Protein that binds and inhibits RAS proteins, which serve as central cell signaling hubs and are essential for the progression of many cancers. Its unique feature is that it does not discriminate between different RAS isoforms or mutations and is capable of binding to RAS in both its active (GTP‐bound) and inactive ...
Jonas N. Kapp, Wouter P. R. Verdurmen, Jonas V. Schaefer, Kari Kopra, Gabriela Nagy‐Davidescu, Elodie Richard, Marie‐Julie Nokin, Patrick Ernst, Rastislav Tamaskovic, Martin Schwill, Ralph Degen, Claudia Scholl, David Santamaria, Andreas Plückthun   +13 more
wiley   +1 more source

Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments [PDF]

, 1984
A.A. Yolov, Е. С. Громова, Е. А. Романова, T. S. Oretskaya, A.A. Oganov, Yа. I. Buryanov, З.А. Шабарова   +6 more
openalex   +1 more source

Tumor clusters with divergent inflammation and human retroelement expression determine the clinical outcome of patients with serous ovarian cancer

Molecular Oncology, EarlyView.
Analysis of treatment‐naïve high‐grade serous ovarian carcinoma (HGSOC) and control tissues for ERVs, LINE‐1 (L1), inflammation, and immune checkpoints identified five clusters with diverse patient recurrence‐free survivals. An inflammation score was calculated and correlated with retroelement expression, where one novel cluster (Triple‐I) with high ...
Laura Glossner, Markus Eckstein, Christoph Mark, Matthias W. Beckmann, Arndt Hartmann, Pamela L. Strissel, Reiner Strick   +6 more
wiley   +1 more source

A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes [PDF]

, 2017
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different Type II enzymes now identified and provides a system for ...
Belfort, Marlene, Bestor, Timothy, Bhagwat, Ashok S., Bickle, Thomas A., Bitinaite, Jurate, Blumenthal, Robert M., Degtyarev, Sergey Kh, Dryden, David T. F., Dybvig, Kevin, Firman, Keith, Gromova, Elizaveta S., Gumport, Richard I., Halford, Stephen E., Hattman, Stanley, Heitman, Joseph, Hornby, David P., Janulaitis, Arvydas, Jeltsch, Albert, Josephsen, Jytte, Kiss, Antal, Klaenhammer, Todd R., Kobayashi, Ichizo, Kong, Huimin, Krüger, Detlev H., Lacks, Sanford, Marinus, Martin G., Miyahara, Michiko, Morgan, Richard D., Murray, Noreen E., Nagaraja, Valakunja, Piekarowicz, Andrzej, Pingoud, Alfred, Raleigh, Elisabeth, Rao, Desirazu N., Reich, Norbert, Repin, Vladimir E., Roberts, Richard J., Selker, Eric U., Shaw, Pang‐Chui, Stein, Daniel C., Stoddard, Barry L., Szybalski, Waclaw, Trautner, Thomas A., Van Etten, James L., Vitor, Jorge M. B., Wilson, Geoffrey G., Xu, Shuang‐yong   +46 more
core  

The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA [PDF]

, 1985
John W. Taylor, Walter Schmidt, Richard Cosstick, And̀rzej Okruszek, Fritz Eckstein   +4 more
openalex   +1 more source