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An in vitro transcription-based protocol for mapping HIV integration sites using lentiviral integration site sequencing. [PDF]
STAR ProtocWięcek K, Wiśniewski J, Chen HC.
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Programmable DNA-Guided Artificial Restriction Enzymes
ACS Synthetic Biology, 2017Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized modern biological research. However, they have limited sequence specificity and availability. Here we report a Pyrococcus furiosus Argonaute (PfAgo) based platform for generating artificial restriction enzymes (AREs) capable of recognizing and cleaving DNA ...
Behnam Enghiad, Huimin Zhao
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DNA Restriction Enzyme from E. coli
Nature, 1968An endonuclease which degrades foreign DNA has been isolated. The enzyme requires S-adenosylmethionine, ATP and Mg++.
M, Meselson, R, Yuan
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Restriction enzyme cleavage of ultraviolet-damaged DNA
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1982SV40 and pBR322 DNAs damaged by ultraviolet light were cleaved abnormally by several restriction enzymes because of damage to pyrimidines in the recognition sequences. The use of a tandemly duplicated plasmid provided a particularly sensitive target molecule for detecting pyrimidine dimers and other possible photoproducts.
J E, Cleaver, L, Samson, G H, Thomas
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Inhibition of DNA restriction enzyme digestion by anthracyclines
La Ricerca in Clinica e in Laboratorio, 1988The inhibition of restriction enzyme digestion of lambda phage DNA by anthracyclines (i.e., adriamycin, daunomycin, epirubicin, idarubicin and esorubicin) commonly used in the treatment of human leukemia and cancer has been studied in vitro. The anthracyclines used inhibit DNA digestion by SmaI, AvaII, HaeIII, HhaI and HpaII, which cut DNA at guanine ...
G. Corneo +3 more
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Restriction Enzyme-Mediated DNA Family Shuffling
2014DNA shuffling is an established recombinatorial method that was originally developed to increase the speed of directed evolution experiments beyond what could be accomplished using error-prone PCR alone. To achieve this, mutated copies of a protein-coding sequence are fragmented with DNase I and the fragments are then reassembled in a PCR without ...
Behrendorff, James B.Y.H. +2 more
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1987
The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
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The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
openaire +1 more source