Results 231 to 240 of about 230,024 (263)
Some of the next articles are maybe not open access.
Structures and mechanisms of DNA restriction and modification enzymes
Quarterly Reviews of Biophysics, 1979DNA restriction and modification enzymes are responsible for the hostspecific barriers to interstrain and interspecies transfer of genetic information that have been observed in a variety of bacterial cell types. Although the phenomenon of host specificity was initially observed in the early 1950s (Luria & Human, 1952; Bertani & Weigle, 1953 ...
openaire +2 more sources
XcmI as a universal restriction enzyme for single-stranded DNA
Gene, 1993Single-stranded DNA can be cleaved into defined fragments at any predetermined site by interaction with a specially designed oligodeoxyribonucleotide (oligo) adaptor and the class-IIN restriction endonuclease, XcmI. The oligo adaptor has the structure [sequence: see text]. Upon hybridization to the target DNA through the central 9-nucleotide region and
P C, Shaw, Y K, Mok
openaire +2 more sources
The manipulation of DNA with restriction enzymes in low water systems
Biochimica et Biophysica Acta (BBA) - General Subjects, 1991The cleavage of phage lambda (lambda) DNA by the restriction enzyme HindIII in low water systems has been investigated. Two types of low water systems have been studied--those which contain a surfactant in a reverse micelle environment and a surfactant-free system in which a solid support (celite) is used.
A B, Hanley +3 more
openaire +2 more sources
DNA fingerprinting of yeast strains by restriction enzyme analysis
Research in Microbiology, 1994Restriction endonuclease analysis was used as a new method to obtain genomic DNA fingerprints in yeast. Fifteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces were examined. Restriction fragments obtained with ApaI or KspI endonucleases were separated by SDS-PAGE and silver-stained.
BARBERIO, CLAUDIA +4 more
openaire +3 more sources
Restriction enzyme analysis of mitochondrial DNA in colorectal tumours
Biochemical and Biophysical Research Communications, 1991Total cellular DNA samples were isolated from 15 colorectal adenocarcinomas, 8 colon adenomas and their adjacent histologically normal colon mucosa. These DNA samples were digested separately with 13 different restriction endonucleases and analysed by Southern blot hybridization using a purified 32P-labelled human mtDNA probe.
W, Cui, I C, Talbot, J M, Northover
openaire +2 more sources
What is the function of DNA restriction enzymes?
Trends in Biochemical Sciences, 1977The message of these figures is that in terms purely of economic cost it would make sense to mount a global vaccina- tion program against smallpox, even if the disease could never be eliminated. The hope of eradicating smallpox altogether rests on the fact that there seems to be no animal reservoir from which the human population can be reinfected, nor
openaire +1 more source
Restriction enzyme studies on human highly repeated DNAs
Experientia, 1982Various restriction enzymes digest human highly repeated homogeneous DNA to discrete fragments, some of which are present in the male and absent in the female. The male specific 2.4 kilobase HaeIII fragment corresponds to human male satellite DNA IV.
G. Corneo +4 more
openaire +3 more sources
An assay for DNA photolyases using non-radioactive DNA and restriction enzymes
Journal of Photochemistry and Photobiology B: Biology, 1993Restriction enzymes, such as Eco RI, Hind III, etc., which have a potential pyrimidine dimer site in their recognition sequence, fail to cleave DNA if their recognition site is modified by the formation of pyrimidine dimers as a result of UV irradiation of DNA (J. E. Cleaver, J. Mol. Biol., 170 (1983) 305-317).
K, Dutta, V S, Hejmadi, N C, Verma
openaire +2 more sources
A continuous electroelution method for the recovery of DNA restriction enzyme fragments
Analytical Biochemistry, 1974Bacterial restriction enzymes are deoxyribonucleases which cleave double-stranded DNA at specific sites and produce unique, high molecular-weight fragments. These enzymes provide a most powerful tool for studying the structure and function of DNA. The principal method for separation of the DNA fragments is electrophoresis through acrylamide or agarose ...
Lee, Amy Shiu, Sinsheimer, Robert L.
openaire +3 more sources
Restriction enzyme mapping of a bacteriophage lambda DNA
Biochemical Education, 1983NOREEN CUNNINGHAM +2 more
openaire +1 more source