Results 221 to 230 of about 230,024 (263)
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On the structure and operation of type I DNA restriction enzymes
Journal of Molecular Biology, 1999Type I DNA restriction enzymes are large, molecular machines possessing DNA methyltransferase, ATPase, DNA translocase and endonuclease activities. The ATPase, DNA translocase and endonuclease activities are specified by the restriction (R) subunit of the enzyme.
David T F Dryden, Noreen E Murray
exaly +3 more sources
Inhibition of DNA restriction enzyme digestion by anthracyclines
La Ricerca in Clinica e in Laboratorio, 1988The inhibition of restriction enzyme digestion of lambda phage DNA by anthracyclines (i.e., adriamycin, daunomycin, epirubicin, idarubicin and esorubicin) commonly used in the treatment of human leukemia and cancer has been studied in vitro. The anthracyclines used inhibit DNA digestion by SmaI, AvaII, HaeIII, HhaI and HpaII, which cut DNA at guanine ...
G. Corneo +3 more
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Restriction enzyme analysis of the Plasmid ColIb DNA
Molecular and General Genetics MGG, 1979Plasmid ColIb (61.5 Mdal) was digested with restriction enzymes EcoRI and HindIII. The DNA digestion products were separated by electrophoresis on 1.2% agarose gels. There were identified 22 fragments of ColIb DNA generated by the endonuclease EcoRI and 21 fragments produced by HindIII. Molecular weights of the fragments were estimated.
A, Skorupska +2 more
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1987
The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
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The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
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Restriction enzyme cleavage of ultraviolet-damaged DNA
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1982SV40 and pBR322 DNAs damaged by ultraviolet light were cleaved abnormally by several restriction enzymes because of damage to pyrimidines in the recognition sequences. The use of a tandemly duplicated plasmid provided a particularly sensitive target molecule for detecting pyrimidine dimers and other possible photoproducts.
J E, Cleaver, L, Samson, G H, Thomas
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DNA Restriction Enzyme from E. coli
Nature, 1968An endonuclease which degrades foreign DNA has been isolated. The enzyme requires S-adenosylmethionine, ATP and Mg++.
M, Meselson, R, Yuan
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Recognition of DNA by Type II Restriction Enzymes
1989Publisher Summary This chapter discusses the recognition of DNA by type II restriction enzymes. A restriction/modification (R/M) system must possess two enzyme activities, the restriction endonuclease and the modification methylase, both of which are dependent on the recognition of the same DNA sequence.
S P, Bennett, S E, Halford
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Restriction Enzyme-Mediated DNA Family Shuffling
2014DNA shuffling is an established recombinatorial method that was originally developed to increase the speed of directed evolution experiments beyond what could be accomplished using error-prone PCR alone. To achieve this, mutated copies of a protein-coding sequence are fragmented with DNase I and the fragments are then reassembled in a PCR without ...
Behrendorff, James B.Y.H. +2 more
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Restriction enzyme analysis of human leukemic mitochondrial DNA
Leukemia Research, 1980Abstract Mitochondrial DNA from both normal human tissue and leukemic human leukocytes (AML and CML) were analyzed by restriction-enzyme digestion, polyacrylamide gradient gel electrophoresis and ethidium bromide staining. Both normal and leukemic human mitochondrial DNA show molecular heterogeneity from individual to individual.
A.M. Gianni, R. Dalla Favera, E. Polli
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Resistance of Tumor-Derived DNA to Restriction Enzyme Digestion
Cancer Investigation, 1990The major finding of this work is that there are specific restriction enzyme inhibitors present in "purified" tumor DNA which cause partial digestion patterns when tumor DNA is digested by standard procedures with any of three commonly employed restriction enzymes (HindIII, KpnI, XbaI).
J L, Parkes, F C, Hubbard, A, Penn
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