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Restriction enzyme analysis of the Plasmid ColIb DNA
Molecular and General Genetics MGG, 1979Plasmid ColIb (61.5 Mdal) was digested with restriction enzymes EcoRI and HindIII. The DNA digestion products were separated by electrophoresis on 1.2% agarose gels. There were identified 22 fragments of ColIb DNA generated by the endonuclease EcoRI and 21 fragments produced by HindIII. Molecular weights of the fragments were estimated.
Anna Skorupska +2 more
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Restriction enzyme cleavage of ultraviolet-damaged DNA
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1982SV40 and pBR322 DNAs damaged by ultraviolet light were cleaved abnormally by several restriction enzymes because of damage to pyrimidines in the recognition sequences. The use of a tandemly duplicated plasmid provided a particularly sensitive target molecule for detecting pyrimidine dimers and other possible photoproducts.
James E. Cleaver, L. Samson, G.H. Thomas
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1987
The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
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The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
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Restriction Enzyme-Mediated DNA Family Shuffling
2014DNA shuffling is an established recombinatorial method that was originally developed to increase the speed of directed evolution experiments beyond what could be accomplished using error-prone PCR alone. To achieve this, mutated copies of a protein-coding sequence are fragmented with DNase I and the fragments are then reassembled in a PCR without ...
Behrendorff, James B.Y.H. +2 more
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Programmable DNA-Guided Artificial Restriction Enzymes
ACS Synthetic Biology, 2017Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized modern biological research. However, they have limited sequence specificity and availability. Here we report a Pyrococcus furiosus Argonaute (PfAgo) based platform for generating artificial restriction enzymes (AREs) capable of recognizing and cleaving DNA ...
Behnam Enghiad, Huimin Zhao
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Recognition of DNA by Type II Restriction Enzymes
1989Publisher Summary This chapter discusses the recognition of DNA by type II restriction enzymes. A restriction/modification (R/M) system must possess two enzyme activities, the restriction endonuclease and the modification methylase, both of which are dependent on the recognition of the same DNA sequence.
Bennett Sp, Halford Se
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Restriction enzyme studies on human highly repeated DNAs
Experientia, 1982Various restriction enzymes digest human highly repeated homogeneous DNA to discrete fragments, some of which are present in the male and absent in the female. The male specific 2.4 kilobase HaeIII fragment corresponds to human male satellite DNA IV.
G. Corneo +4 more
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Restriction enzyme analysis of human leukemic mitochondrial DNA
Leukemia Research, 1980Abstract Mitochondrial DNA from both normal human tissue and leukemic human leukocytes (AML and CML) were analyzed by restriction-enzyme digestion, polyacrylamide gradient gel electrophoresis and ethidium bromide staining. Both normal and leukemic human mitochondrial DNA show molecular heterogeneity from individual to individual.
A.M. Gianni, R. Dalla Favera, E. Polli
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DNA fingerprinting of yeast strains by restriction enzyme analysis
Research in Microbiology, 1994Restriction endonuclease analysis was used as a new method to obtain genomic DNA fingerprints in yeast. Fifteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces were examined. Restriction fragments obtained with ApaI or KspI endonucleases were separated by SDS-PAGE and silver-stained.
BARBERIO, CLAUDIA +4 more
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Structures and mechanisms of DNA restriction and modification enzymes
Quarterly Reviews of Biophysics, 1979DNA restriction and modification enzymes are responsible for the hostspecific barriers to interstrain and interspecies transfer of genetic information that have been observed in a variety of bacterial cell types. Although the phenomenon of host specificity was initially observed in the early 1950s (Luria & Human, 1952; Bertani & Weigle, 1953 ...
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