Results 311 to 320 of about 390,387 (330)
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Restriction enzyme analysis of human leukemic mitochondrial DNA
Leukemia Research, 1980Abstract Mitochondrial DNA from both normal human tissue and leukemic human leukocytes (AML and CML) were analyzed by restriction-enzyme digestion, polyacrylamide gradient gel electrophoresis and ethidium bromide staining. Both normal and leukemic human mitochondrial DNA show molecular heterogeneity from individual to individual.
A.M. Gianni, R. Dalla Favera, E. Polli
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DNA fingerprinting of yeast strains by restriction enzyme analysis
Research in Microbiology, 1994Restriction endonuclease analysis was used as a new method to obtain genomic DNA fingerprints in yeast. Fifteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces were examined. Restriction fragments obtained with ApaI or KspI endonucleases were separated by SDS-PAGE and silver-stained.
BARBERIO, CLAUDIA +4 more
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Structures and mechanisms of DNA restriction and modification enzymes
Quarterly Reviews of Biophysics, 1979DNA restriction and modification enzymes are responsible for the hostspecific barriers to interstrain and interspecies transfer of genetic information that have been observed in a variety of bacterial cell types. Although the phenomenon of host specificity was initially observed in the early 1950s (Luria & Human, 1952; Bertani & Weigle, 1953 ...
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What is the function of DNA restriction enzymes?
Trends in Biochemical Sciences, 1977The message of these figures is that in terms purely of economic cost it would make sense to mount a global vaccina- tion program against smallpox, even if the disease could never be eliminated. The hope of eradicating smallpox altogether rests on the fact that there seems to be no animal reservoir from which the human population can be reinfected, nor
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Resistance of Tumor-Derived DNA to Restriction Enzyme Digestion
Cancer Investigation, 1990The major finding of this work is that there are specific restriction enzyme inhibitors present in "purified" tumor DNA which cause partial digestion patterns when tumor DNA is digested by standard procedures with any of three commonly employed restriction enzymes (HindIII, KpnI, XbaI).
Joan Lee Parkes +2 more
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Restriction enzyme analysis of mitochondrial DNA in colorectal tumours
Biochemical and Biophysical Research Communications, 1991Total cellular DNA samples were isolated from 15 colorectal adenocarcinomas, 8 colon adenomas and their adjacent histologically normal colon mucosa. These DNA samples were digested separately with 13 different restriction endonucleases and analysed by Southern blot hybridization using a purified 32P-labelled human mtDNA probe.
Wei Cui +2 more
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On the structure and operation of type I DNA restriction enzymes
Journal of Molecular Biology, 1999Type I DNA restriction enzymes are large, molecular machines possessing DNA methyltransferase, ATPase, DNA translocase and endonuclease activities. The ATPase, DNA translocase and endonuclease activities are specified by the restriction (R) subunit of the enzyme.
David T. F. Dryden +5 more
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The manipulation of DNA with restriction enzymes in low water systems
Biochimica et Biophysica Acta (BBA) - General Subjects, 1991The cleavage of phage lambda (lambda) DNA by the restriction enzyme HindIII in low water systems has been investigated. Two types of low water systems have been studied--those which contain a surfactant in a reverse micelle environment and a surfactant-free system in which a solid support (celite) is used.
Caroline S.M. Furniss +3 more
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XcmI as a universal restriction enzyme for single-stranded DNA
Gene, 1993Single-stranded DNA can be cleaved into defined fragments at any predetermined site by interaction with a specially designed oligodeoxyribonucleotide (oligo) adaptor and the class-IIN restriction endonuclease, XcmI. The oligo adaptor has the structure [sequence: see text]. Upon hybridization to the target DNA through the central 9-nucleotide region and
Yu-Keung Mok, Pang-Chui Shaw
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Restriction enzyme mapping of a bacteriophage lambda DNA [PDF]
Biochemical Education, 1983 Frances Jurnak +2 more
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