Results 11 to 20 of about 3,728,691 (365)
Evaluating the analytical validity of circulating tumor DNA sequencing assays for precision oncology
Nature Biotechnology, 2021 Circulating tumor DNA (ctDNA) sequencing is being rapidly adopted in precision oncology, but the accuracy, sensitivity and reproducibility of ctDNA assays is poorly understood. Here we report the findings of a multi-site, cross-platform evaluation of the
I. Deveson +85 more
semanticscholar +1 more source
Comparison of mitochondrial DNA sequences from whole blood and lymphoblastoid cell lines
Scientific Reports, 2022 Lymphoblastoid cell lines (LCLs) provide an unlimited source of genomic DNA for genetic studies. Here, we compared mtDNA sequence variants, heteroplasmic or homplasmic, between LCL (sequenced by mitoRCA-seq method) and whole blood samples (sequenced ...
Chunyu Liu +8 more
doaj +1 more source
BMC Medical Genomics, 2021 Background Coronary artery calcification (CAC) is a noninvasive measure of coronary atherosclerosis, the proximal pathophysiology underlying most cases of myocardial infarction (MI). We sought to identify expression signatures of early MI and subclinical
Xiaoling Zhang +13 more
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Applications of Single-Cell DNA Sequencing.
Annual review of genomics and human genetics (Print), 2021 Over the past decade, genomic analyses of single cells-the fundamental units of life-have become possible. Single-cell DNA sequencing has shed light on biological questions that were previously inaccessible across diverse fields of research, including ...
Gilad D. Evrony, A. Hinch, C. Luo
semanticscholar +1 more source
BARCOSEL: a tool for selecting an optimal barcode set for high-throughput sequencing
BMC Bioinformatics, 2018 Background Current high-throughput sequencing platforms provide capacity to sequence multiple samples in parallel. Different samples are labeled by attaching a short sample specific nucleotide sequence, barcode, to each DNA molecule prior pooling them ...
Panu Somervuo +5 more
doaj +1 more source
Infectious Diseases and Therapy, 2023 Introduction The introduction of pneumococcal conjugate vaccines (PCV) into the national immunization programs (NIPs) has significantly reduced the number of pneumococcal infections.
Weronika Puzia +9 more
doaj +1 more source
Enzymatic methyl sequencing detects DNA methylation at single-base resolution from picograms of DNA
Genome Research, 2021 Bisulfite sequencing detects 5mC and 5hmC at single-base resolution. However, bisulfite treatment damages DNA, which results in fragmentation, DNA loss, and biased sequencing data.
Romualdas Vaisvila +19 more
semanticscholar +1 more source
Genome Research, 2010 Next-generation DNA sequencing (NGS) projects, such as the 1000 Genomes Project, are already revolutionizing our understanding of genetic variation among individuals.
A. McKenna +10 more
semanticscholar +1 more source
The sequence of sequencers: The history of sequencing DNA
Genomics, 2016 Determining the order of nucleic acid residues in biological samples is an integral component of a wide variety of research applications. Over the last fifty years large numbers of researchers have applied themselves to the production of techniques and technologies to facilitate this feat, sequencing DNA and RNA molecules. This time-scale has witnessed
Heather, JM, Chain, B
openaire +4 more sources
The microbiome of the human lower airways: a next generation sequencing perspective
World Allergy Organization Journal, 2015 For a long time, the human lower airways were considered a sterile environment where the presence of microorganisms, typically revealed by culturing, was interpreted as an abnormal health state.
Velma T.E. Aho +5 more
doaj +1 more source