Results 191 to 200 of about 1,188,878 (324)

Effects of different DNA polymerases in ligation-mediated PCR: enhanced genomic sequencing and in vivo footprinting. [PDF]

, 1992
Paul Garrity, B Wold
openalex   +1 more source

Ubiquitination of transcription factors in cancer: unveiling therapeutic potential

Molecular Oncology, EarlyView.
In cancer, dysregulated ubiquitination of transcription factors contributes to the uncontrolled growth and survival characteristics of tumors. Tumor suppressors are degraded by aberrant ubiquitination, or oncogenic transcription factors gain stability through ubiquitination, thereby promoting tumorigenesis.
Dongha Kim, Hye Jin Nam, Sung Hee Baek
wiley   +1 more source

Proviral HIV DNA sequencing to inform suitability for long-acting injectable cabotegravir/rilpivirine. [PDF]

AIDS
Alagaratnam J, Kelly N, Dustan S, Naous N, Nwanbila M, Byrne R, Girometti N, Asboe D, Taylor GP, Boffito M.   +9 more
europepmc   +1 more source

Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas [PDF]

, 1994
Vivek Kapur, L L Li, S Iordănescu, Marcie R. Hamrick, Audrey Wanger, Barry N. Kreiswirth, James M. Musser   +6 more
openalex   +1 more source

A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.

, 1992
Marianne Frommer, Louise E. McDonald, David Millar, Christina M. Collis, Fujiko Watt, G. W. GRIGG, Peter L. Molloy, Cheryl Paul   +7 more
openalex   +1 more source

Expression and purification of E140 protein antigen fragments of Plasmodium vivax and Plasmodium berghei for serological assays

FEBS Open Bio, EarlyView.
We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques, Edit Ábrahám, Hiromi Muramatsu, Daniel Youssef Bargieri, Norbert Pardi, Zoltán Lipinszki   +5 more
wiley   +1 more source

Phylogenetic analysis of the family Rhizobiaceae and related bacteria by sequencing of 16S rRNA gene using PCR and DNA sequencer [PDF]

, 1993
M Yanagi
openalex   +1 more source

FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production

FEBS Open Bio, EarlyView.
FAM136A depletion upregulated ROS production, reduced mitochondrial membrane potential (ΔΨ) and ATP production, and upregulated expression of PCK1, PCK2, HMGCS1, and HMGCS2. The expression of both TOMM22 and TOMM20 was also upregulated. FAM136A depletion reduced HCCS that produce holocytochrome c by combining heme to apocytochrome c, and reduced the ...
Yushi Otsuka, Masato Yano
wiley   +1 more source

Retrospective analysis of 300 microbial cell-free DNA sequencing results in routine blood stream infection diagnostics. [PDF]

Front Cell Infect Microbiol
Neidhöfer C, Klein N, Yürüktümen A, Hattenhauer T, Mispelbaum R, Bode C, Holderried TAW, Hoerauf A, Parčina M.   +8 more
europepmc   +1 more source

Pharmacological effects of osimertinib on a chicken chorioallantoic membrane xenograft model with the EGFR exon‐19‐deleted advanced NSCLC mutation

FEBS Open Bio, EarlyView.
Osimertinib reduces angiogenesis and PDL1 expression in in ovo tumors, transforming them into ‘cold tumors’ with lower immune activity. Anatomopathological and transcriptomic analyses highlight its therapeutic impact on tumor biology. This study underscores osimertinib's potential to reshape the tumor microenvironment and provides insights into its ...
David Barthélémy, Arnaud Vigneron, Xavier Rousset, Jérome Guitton, Emmanuel Grolleau, Margaux Raffin, Julie Balandier, Gaëlle Lescuyer, Mathilde Bardou, Florence Geiguer, Sébastien Couraud, Claire Bardel, Jean Viallet, Nazim Benzerdjeb, Léa Payen   +14 more
wiley   +1 more source