Results 21 to 30 of about 3,728,691 (365)

Case Report of COVID-19 after Full Vaccination: Viral Loads and Anti-SARS-CoV-2 Antibodies

Diagnostics, 2021
The introduction of effective vaccines against SARS-CoV-2 is expected to prevent COVID-19. However, sporadic cases of infection in vaccinated persons have been reported. We describe a case of a double-dose vaccinated woman with COVID-19.
Magdalena Komiazyk, Jaroslaw Walory, Jan Gawor, Iza Ksiazek, Robert Gromadka, Anna Baraniak   +5 more
doaj   +1 more source

A framework for variation discovery and genotyping using next-generation DNA sequencing data

Nature Genetics, 2011
Recent advances in sequencing technology make it possible to comprehensively catalog genetic variation in population samples, creating a foundation for understanding human disease, ancestry and evolution.
M. DePristo, E. Banks, R. Poplin, K. Garimella, J. Maguire, C. Hartl, A. Philippakis, G. Del Angel, M. Rivas, M. Hanna, A. McKenna, T. Fennell, A. Kernytsky, A. Sivachenko, K. Cibulskis, S. Gabriel, D. Altshuler, M. Daly   +17 more
semanticscholar   +1 more source

Enhanced detection of minimal residual disease by targeted sequencing of phased variants in circulating tumor DNA

Nature Biotechnology, 2021
Circulating tumor-derived DNA (ctDNA) is an emerging biomarker for many cancers, but the limited sensitivity of current detection methods reduces its utility for diagnosing minimal residual disease.
D. Kurtz, Joanne Soo, Lyron Co Ting Keh, S. Alig, J. Chabon, B. Sworder, A. Schultz, M. Jin, F. Scherer, A. Garofalo, C. Macaulay, Emily G. Hamilton, Binbin Chen, M. Olsen, J. Schroers-Martin, A. Craig, E. Moding, M. S. Esfahani, C. Liu, U. Dührsen, A. Hüttmann, R. Casasnovas, J. Westin, M. Roschewski, W. Wilson, G. Gaidano, D. Rossi, M. Diehn, Ash A. Alizadeh   +28 more
semanticscholar   +1 more source

Discovering multiple types of DNA methylation from bacteria and microbiome using nanopore sequencing

Nature Methods, 2021
Bacterial DNA methylation occurs at diverse sequence contexts and plays important functional roles in cellular defense and gene regulation. Existing methods for detecting DNA modification from nanopore sequencing data do not effectively support de novo ...
Alan Tourancheau, E. Mead, Xue-Song Zhang, Gang Fang   +3 more
semanticscholar   +1 more source

Exoquence DNA sequencing [PDF]

Nucleic Acids Research, 1993
We have developed a strategy for DNA sequencing based on exonuclease III digestion followed by double strand specific endonuclease digestion and direct dideoxynucleotide sequencing reaction. This strategy eliminates the need for subcloning, oligonucleotide primers, and prior knowledge of the DNA to be sequenced.
Chuan Li, Philip W. Tucker
openaire   +3 more sources

A first insight into the genome of Prototheca wickerhamii, a major causative agent of human protothecosis

BMC Genomics, 2021
Background Colourless microalgae of the Prototheca genus are the only known plants that have consistently been implicated in a range of clinically relevant opportunistic infections in both animals and humans.
Zofia Bakuła, Paweł Siedlecki, Robert Gromadka, Jan Gawor, Agnieszka Gromadka, Jan J. Pomorski, Hanna Panagiotopoulou, Tomasz Jagielski   +7 more
doaj   +1 more source

DNA methylation-calling tools for Oxford Nanopore sequencing: a survey and human epigenome-wide evaluation

Genome Biology, 2021
Nanopore long-read sequencing technology greatly expands the capacity of long-range, single-molecule DNA-modification detection. A growing number of analytical tools have been developed to detect DNA methylation from nanopore sequencing reads.
Yang Liu, Wojciech Rosikiewicz, Ziwei Pan, Nathaniel Jillette, Ping Wang, A. Taghbalout, J. Foox, C. Mason, M. Carroll, A. Cheng, Sheng Li   +10 more
semanticscholar   +1 more source

An optimized method for high quality DNA extraction from microalga Prototheca wickerhamii for genome sequencing

Plant Methods, 2017
Background The complex cell wall structure of algae often precludes efficient extraction of their genetic material. The purpose of this study was to design a next-generation sequencing-suitable DNA isolation method for unicellular, achlorophyllous, yeast-
Tomasz Jagielski, Jan Gawor, Zofia Bakuła, Karolina Zuchniewicz, Iwona Żak, Robert Gromadka   +5 more
doaj   +1 more source

Bacterial Communities Associated with Poa annua Roots in Central European (Poland) and Antarctic Settings (King George Island)

Microorganisms, 2021
Poa annua (annual bluegrass) is one of the most ubiquitous grass species in the world. In isolated regions of maritime Antarctica, it has become an invasive organism threatening native tundra communities.
Anna Znój, Jakub Grzesiak, Jan Gawor, Robert Gromadka, Katarzyna J. Chwedorzewska   +4 more
doaj   +1 more source

Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads

bioRxiv, 2016
The Illumina DNA sequencing platform generates accurate but short reads, which can be used to produce accurate but fragmented genome assemblies. Pacific Biosciences and Oxford Nanopore Technologies DNA sequencing platforms generate long reads that can ...
R. Wick, L. Judd, C. Gorrie, K. Holt
semanticscholar   +1 more source