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Current Protocols in Molecular Biology, 2006
AbstractPolony DNA sequencing provides an inexpensive, accurate, high‐throughput way to resequence genomes of interest by comparison to a reference genome. Mate‐paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on
George M. Church +2 more
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AbstractPolony DNA sequencing provides an inexpensive, accurate, high‐throughput way to resequence genomes of interest by comparison to a reference genome. Mate‐paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on
George M. Church +2 more
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Analytical Chemistry, 1995
Faster methods than are currently available will be needed to sequence the billions of base pairs of DNA in the human genome in a reasonable number of years and without exhausting available funds. A new sequencing technology in which DNA binds or hybridizes' to an array of oligonucleotides on a silicon chip appears to be promising as a high-throughput ...
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Faster methods than are currently available will be needed to sequence the billions of base pairs of DNA in the human genome in a reasonable number of years and without exhausting available funds. A new sequencing technology in which DNA binds or hybridizes' to an array of oligonucleotides on a silicon chip appears to be promising as a high-throughput ...
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2003
The polymerase chain reaction (PCR), first published by Mullis and Faloona in 1987 (1), has become an invaluable molecular biology tool in both research and routine applications. The combination of PCR with the chain-termination sequencing technique developed by Sanger et al.
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The polymerase chain reaction (PCR), first published by Mullis and Faloona in 1987 (1), has become an invaluable molecular biology tool in both research and routine applications. The combination of PCR with the chain-termination sequencing technique developed by Sanger et al.
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2010
Fluorescent cycle sequencing of PCR products is a multistage process and several methodologies are available to perform each stage. This chapter will describe the more commonly utilised dye-terminator cycle sequencing approach using BigDye® terminator chemistry (Applied Biosystems) ready for analysis on a 3730 DNA genetic analyzer.
Natalie Morrell, Yvonne Wallis
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Fluorescent cycle sequencing of PCR products is a multistage process and several methodologies are available to perform each stage. This chapter will describe the more commonly utilised dye-terminator cycle sequencing approach using BigDye® terminator chemistry (Applied Biosystems) ready for analysis on a 3730 DNA genetic analyzer.
Natalie Morrell, Yvonne Wallis
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Statistical Methods in Medical Research, 1993
Recent developments in the statistical analysis of DNA sequences are reviewed. The pace with which sequence data are being generated and analysed has increased with the growth of the human genome project. Two areas of activity are emphasized: attention to error rates in recorded sequences, and heterogeneity in structure of sequences.
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Recent developments in the statistical analysis of DNA sequences are reviewed. The pace with which sequence data are being generated and analysed has increased with the growth of the human genome project. Two areas of activity are emphasized: attention to error rates in recorded sequences, and heterogeneity in structure of sequences.
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Next-generation DNA sequencing
Nature Biotechnology, 2008J. Shendure, Hanlee P. Ji
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Science, 1999
The News Focus article by Eliot Marshall titled “A high-stakes gamble on genome sequencing” (18 June, p. [1906][1]) states that the PE Biosystems model 3700 DNA sequencer was developed by industry. While PE Biosystems invested significant resources in their outstanding engineering work for the instrument, the original prototypes were developed by H ...
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The News Focus article by Eliot Marshall titled “A high-stakes gamble on genome sequencing” (18 June, p. [1906][1]) states that the PE Biosystems model 3700 DNA sequencer was developed by industry. While PE Biosystems invested significant resources in their outstanding engineering work for the instrument, the original prototypes were developed by H ...
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Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods in Enzymology, 1980A. Maxam, W. Gilbert
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