Results 31 to 40 of about 1,811,241 (359)
Defects Can Increase the Melting Temperature of DNA-Nanoparticle Assemblies [PDF]
J. Phys. Chem. B, 110 (2006) 16393-16396, 2007 DNA-gold nanoparticle assemblies have shown promise as an alternative technology to DNA microarrays for DNA detection and RNA profiling. Understanding the effect of DNA sequences on the melting temperature of the system is central to developing reliable detection technology.
arxiv +1 more source
Identification of single nucleotides in MoS2 nanopores [PDF]
, 2015 Ultrathin membranes have drawn much attention due to their unprecedented spatial resolution for DNA nanopore sequencing. However, the high translocation velocity (3000-50000 nt/ms) of DNA molecules moving across such membranes limits their usability.
Bulushev, Roman D. +6 more
core +2 more sources
ELECTROPHORESIS, 2009 AbstractSequencing of human and other genomes has been at the center of interest in the biomedical field over the past several decades and is now leading toward an era of personalized medicine. During this time, DNA‐sequencing methods have evolved from the labor‐intensive slab gel electrophoresis, through automated multiCE systems using fluorophore ...
András Guttman, Barry L. Karger
openaire +3 more sources
Molecular Therapy: Methods & Clinical Development, 2022 With the aim of expediting drug target discovery and validation for respiratory diseases, we developed an optimized method for in situ somatic gene disruption in murine lung epithelial cells via AAV6-mediated CRISPR-Cas9 delivery.
Honglin Chen +13 more
doaj
Highly accurate long reads are crucial for realizing the potential of biodiversity genomics
BMC Genomics, 2023 Background Generating the most contiguous, accurate genome assemblies given available sequencing technologies is a long-standing challenge in genome science.
Scott Hotaling +4 more
doaj +1 more source
Functionalized nanopore-embedded electrodes for rapid DNA sequencing [PDF]
, 2007 The determination of a patient's DNA sequence can, in principle, reveal an increased risk to fall ill with particular diseases [1,2] and help to design "personalized medicine" [3].
Ahuja, Rajeev +7 more
core +1 more source
Bisulfite Sequencing of DNA [PDF]
Current Protocols in Molecular Biology, 2010 AbstractExact positions of 5‐methylcytosine (m5C) on a single strand of DNA can be determined by bisulfite genomic sequencing (BGS). Treatment with bisulfite ion preferentially deaminates unmethylated cytosines, which are then converted to uracil upon desulfonation.
Russell P. Darst +4 more
openaire +3 more sources
Complete mitochondrial genome of the freshwater prawn Palaemon capensis (Crustacea: Palaemonidae)
Mitochondrial DNA. Part B. Resources, 2017 The complete mitogenome of Palaemon capensis is presented here. The mitogenome is 15,925 bp in length and comprises 13 protein coding genes, 2 ribosomal subunit genes, 22 transfer RNAs, and a non-coding AT-rich region.
Louisa E. Wood +3 more
doaj +1 more source
Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]
, 2019 IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
Ackerman, A Lenore +8 more
core +3 more sources
A new species of Xenoturbella from the western Pacific Ocean and the evolution of Xenoturbella
BMC Evolutionary Biology, 2017 Background Xenoturbella is a group of marine benthic animals lacking an anus and a centralized nervous system. Molecular phylogenetic analyses group the animal together with the Acoelomorpha, forming the Xenacoelomorpha.
Hiroaki Nakano +9 more
doaj +1 more source