Results 71 to 80 of about 3,677,492 (346)
Molecular genetic features of the development of restrictive cardiomyopathy in Russian children
Российский кардиологический журнал, 2021 Aim. To identify the proportion of restrictive cardiomyopathy (RCM), as well as cardiomyopathy (CMP) with a restrictive type of hemodynamics among all cases of genetic CMP and to determine the relative frequencies and spectrum of nucleotide variants in ...
K. V. Savostyanov +9 more
doaj +1 more source
A new method for sequencing DNA.
Proceedings of the National Academy of Sciences of the United States of America, 1977 DNA can be sequenced by a chemical procedure that breaks a terminally labeled DNA molecule partially at each repetition of a base. The lengths of the labeled fragments then identify the positions of that base.
A. Maxam, W. Gilbert
semanticscholar +1 more source
Evolutionary interplay between viruses and R‐loops
FEBS Letters, EarlyView.Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi +4 more
wiley +1 more source
Circulating Tumor DNA Sequencing Analysis of Gastroesophageal Adenocarcinoma
Clinical Cancer Research, 2019 Purpose: Gastroesophageal adenocarcinoma (GEA) has a poor prognosis and few therapeutic options. Utilizing a 73-gene plasma-based next-generation sequencing (NGS) cell-free circulating tumor DNA (ctDNA-NGS) test, we sought to evaluate the role of ctDNA ...
S. Maron +13 more
semanticscholar +1 more source
Circulating histones as clinical biomarkers in critically ill conditions
FEBS Letters, EarlyView.Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez +17 more
wiley +1 more source
Increasing the accuracy of nanopore DNA sequencing using a time-varying cross membrane voltage
Nature Biotechnology, 2019 Nanopore DNA sequencing is limited by low base-calling accuracy. Improved base-calling accuracy has so far relied on specialized base-calling algorithms, different nanopores and motor enzymes, or biochemical methods to re-read DNA molecules.
Matthew Travis Noakes +10 more
semanticscholar +1 more source
FEBS Letters, EarlyView.This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao +7 more
wiley +1 more source
JAMA Oncology, 2019 This pilot cohort study estimates sensitivity and specificity of plasma microbial cell-free DNA sequencing for its ability to predict bloodstream infection in patients at high risk of life-threatening infection.
Kathryn P Goggin +18 more
semanticscholar +1 more source
FEBS Letters, EarlyView.In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi +5 more
wiley +1 more source
High-intensity sequencing reveals the sources of plasma circulating cell-free DNA variants
Nature Network Boston, 2019 Accurate identification of tumor-derived somatic variants in plasma circulating cell-free DNA (cfDNA) requires understanding of the various biological compartments contributing to the cfDNA pool.
P. Razavi +42 more
semanticscholar +1 more source