Results 241 to 250 of about 34,995 (285)
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DNA shuffling of subgenomic sequences of subtilisin
Nature Biotechnology, 1999DNA family shuffling of 26 protease genes was used to create a library of chimeric proteases that was screened for four distinct enzymatic properties. Multiple clones were identified that were significantly improved over any of the parental enzymes for each individual property. Family shuffling, also known as molecular breeding, efficiently created all
J E, Ness +7 more
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Restriction Enzyme-Mediated DNA Family Shuffling
2014DNA shuffling is an established recombinatorial method that was originally developed to increase the speed of directed evolution experiments beyond what could be accomplished using error-prone PCR alone. To achieve this, mutated copies of a protein-coding sequence are fragmented with DNase I and the fragments are then reassembled in a PCR without ...
Behrendorff, James B.Y.H. +2 more
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Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineering
2016DNA shuffling is a powerful tool to develop libraries of variants for protein engineering. Here, we present a protocol to use our freely available and easy-to-use computer program, Shuffle Optimizer. Shuffle Optimizer is written in the Python computer language and increases the nucleotide homology between two pieces of DNA desired to be shuffled ...
John N, Milligan, Daniel J, Garry
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DNA ALGORITHMS BASED ON EXON SHUFFLING
IFAC Proceedings Volumes, 2005Abstract An understanding of a natural system's information handling can lead to more effective artificial optimization techniques. There are successful optimization algorithms represented in biosystems that have proven useful in engineering applications (artificial neural networks, immune system algorithms, etc).
Tsuyoshi Okayama, Haruhiko Murase
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Evolution of a cytokine using DNA family shuffling
Nature Biotechnology, 1999DNA shuffling of a family of over 20 human interferon-alpha (Hu-IFN-alpha) genes was used to derive variants with increased antiviral and antiproliferation activities in murine cells. A clone with 135,000-fold improved specific activity over Hu-IFN-alpha2a was obtained in the first cycle of shuffling.
C C, Chang +6 more
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Current opinion in molecular therapeutics, 2001
One challenge of biotechnology is to find ways to optimize enzymes, cytokines, vaccines or transgenes in new contexts that are typically not found in nature. The approach of DNA shuffling is a test-tube process that takes advantage of recombination to generate libraries of chimeric genes, which can then be screened to identify the encoded proteins ...
R G, Whalen +3 more
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One challenge of biotechnology is to find ways to optimize enzymes, cytokines, vaccines or transgenes in new contexts that are typically not found in nature. The approach of DNA shuffling is a test-tube process that takes advantage of recombination to generate libraries of chimeric genes, which can then be screened to identify the encoded proteins ...
R G, Whalen +3 more
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Tailoring vectors through DNA shuffling
Nature Genetics, 2000Rational vector design is pivotal for successful human gene therapy and the application of high-throughput methods may provide a means for the efficient realization of vectors with desired properties. A method involving DNA shuffling represents a powerful new paradigm in this regard.
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Applications of DNA shuffling to pharmaceuticals and vaccines
Current Opinion in Biotechnology, 1997DNA shuffling is a practical process for directed molecular evolution which uses recombination to dramatically accelerate the rate at which one can evolve genes. Single and multigene traits that require many mutations for improved phenotypes can be evolved rapidly. DNA shuffling technology has been significantly enhanced in the past year, extending its
P A, Patten, R J, Howard, W P, Stemmer
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Predicting Out-of-Sequence Reassembly in DNA Shuffling
Journal of Theoretical Biology, 2002We present an analysis for calculating the frequency of out-of-sequence reassembly in DNA shuffling experiments. Out-of-sequence annealing events are undesirable since they typically encode non-functional proteins with missing or repetitive regions.
Gregory L, Moore, Costas D, Maranas
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Retroviral DNA integration: lessons for transposon shuffling
Gene, 1993Phylogenetic comparisons of retroviral IN (integrase) protein sequences have revealed homologies that extend to the retrotransposon and bacterial transposase families and have provided evidence for at least two functional domains. The N-terminal region is characterized by a Zn-finger-like array which is conserved in retrotransposons. The central region
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