Results 221 to 230 of about 17,272 (247)

Restriction Enzyme-Mediated DNA Family Shuffling

2014
DNA shuffling is an established recombinatorial method that was originally developed to increase the speed of directed evolution experiments beyond what could be accomplished using error-prone PCR alone. To achieve this, mutated copies of a protein-coding sequence are fragmented with DNase I and the fragments are then reassembled in a PCR without ...
Behrendorff, James B.Y.H., Johnston, Wayne A., Gillam, Elizabeth M.J.   +2 more
openaire   +5 more sources

DNA ALGORITHMS BASED ON EXON SHUFFLING

IFAC Proceedings Volumes, 2005
Abstract An understanding of a natural system's information handling can lead to more effective artificial optimization techniques. There are successful optimization algorithms represented in biosystems that have proven useful in engineering applications (artificial neural networks, immune system algorithms, etc).
Tsuyoshi Okayama, Haruhiko Murase
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Retroviral DNA integration: lessons for transposon shuffling

Gene, 1993
Phylogenetic comparisons of retroviral IN (integrase) protein sequences have revealed homologies that extend to the retrotransposon and bacterial transposase families and have provided evidence for at least two functional domains. The N-terminal region is characterized by a Zn-finger-like array which is conserved in retrotransposons. The central region
openaire   +2 more sources

Development of metagenomic DNA shuffling for the construction of a xenobiotic gene

Gene, 2006
We describe a metagenomic DNA shuffling process by combining protein engineering process mutation generator and the high potential diversity of metagenomic DNA derived from the environment. Numerous previous shuffling processes attempted to recombine more or less related parental sequences.
Boubakri, Hasna, Beuf, M., Simonet, Pascal, Vogel, Timothy   +3 more
openaire   +3 more sources

Molecular evolution of an arsenate detoxification pathway by DNA shuffling

Nature Biotechnology, 1997
Functional evolution of an arsenic resistance operon has been accomplished by DNA shuffling, involving multiple rounds of in vitro recombination and mutation of a pool of related sequences, followed by selection for increased resistance in vivo.
A, Crameri, G, Dawes, E, Rodriguez, S, Silver, W P, Stemmer   +4 more
openaire   +2 more sources

DNA Shuffling for Whole Cell Engineering

2003
Pasteur patented the use of whole microbial cells in fermentation in the latter part of the nineteenth century. However, there are much earlier references to microbial fermentations, dating back to alcohol fermentation thousands of years ago. Although more recent times have seen the introduction of many novel processes.
Steve del Cardayre, Keith Powell
openaire   +1 more source

Tailoring vectors through DNA shuffling

Nature Genetics, 2000
Rational vector design is pivotal for successful human gene therapy and the application of high-throughput methods may provide a means for the efficient realization of vectors with desired properties. A method involving DNA shuffling represents a powerful new paradigm in this regard.
openaire   +1 more source

DNA Shuffling and Family Shuffling for In Vitro Gene Evolution

2002
Miho, Kikuchi, Shigeaki, Harayama
openaire   +2 more sources

Family Shuffling with Single-Stranded DNA

2003
Wenjuan, Zha, Tongbo, Zhu, Huimin, Zhao
openaire   +2 more sources

DNA droplets shuffle in the spotlight

Nature Materials
Brigitta Dúzs, Andreas Walther
openaire   +2 more sources