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Proceedings of the second annual international conference on Computational molecular biology - RECOMB '98, 1998
In vitro evolution is a new, important laboratory method to evolve molecules with desired properties. It has been used in a variety of biological studies and drug development. In this paper, we study one important mutagenesis method used in in vitro evolution experiments called DNA shuffling.
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In vitro evolution is a new, important laboratory method to evolve molecules with desired properties. It has been used in a variety of biological studies and drug development. In this paper, we study one important mutagenesis method used in in vitro evolution experiments called DNA shuffling.
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DNA shuffling of subgenomic sequences of subtilisin
Nature Biotechnology, 1999DNA family shuffling of 26 protease genes was used to create a library of chimeric proteases that was screened for four distinct enzymatic properties. Multiple clones were identified that were significantly improved over any of the parental enzymes for each individual property. Family shuffling, also known as molecular breeding, efficiently created all
J E, Ness +7 more
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DNA Shuffling of Cytochrome P450 Enzymes
2013DNA family shuffling is an efficient method for creating libraries of novel enzymes, in which a high proportion of mutants exhibit correct folding and possess catalytic properties distinct from the starting material. The evolutionary arrangement of cytochromes P450 into subfamilies of enzymes with highly similar nucleotide sequences but distinct ...
Behrendorff, James B.Y.H. +2 more
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Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineering
2016DNA shuffling is a powerful tool to develop libraries of variants for protein engineering. Here, we present a protocol to use our freely available and easy-to-use computer program, Shuffle Optimizer. Shuffle Optimizer is written in the Python computer language and increases the nucleotide homology between two pieces of DNA desired to be shuffled ...
John N, Milligan, Daniel J, Garry
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Evolution of a cytokine using DNA family shuffling
Nature Biotechnology, 1999DNA shuffling of a family of over 20 human interferon-alpha (Hu-IFN-alpha) genes was used to derive variants with increased antiviral and antiproliferation activities in murine cells. A clone with 135,000-fold improved specific activity over Hu-IFN-alpha2a was obtained in the first cycle of shuffling.
C C, Chang +6 more
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Single-Stranded DNA Family Shuffling
2004Publisher Summary This chapter examines the various aspects of single-stranded DNA family shuffling. DNA shuffling is a technique that allows accelerated and directed protein evolution in vitro . This technique has been shown to be powerful in combining independently isolated mutations of a gene into a single progeny. By using the sense strand ssDNA
Osamu, Kagami +2 more
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Predicting Out-of-Sequence Reassembly in DNA Shuffling
Journal of Theoretical Biology, 2002We present an analysis for calculating the frequency of out-of-sequence reassembly in DNA shuffling experiments. Out-of-sequence annealing events are undesirable since they typically encode non-functional proteins with missing or repetitive regions.
Gregory L, Moore, Costas D, Maranas
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Current opinion in molecular therapeutics, 2001
One challenge of biotechnology is to find ways to optimize enzymes, cytokines, vaccines or transgenes in new contexts that are typically not found in nature. The approach of DNA shuffling is a test-tube process that takes advantage of recombination to generate libraries of chimeric genes, which can then be screened to identify the encoded proteins ...
R G, Whalen +3 more
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One challenge of biotechnology is to find ways to optimize enzymes, cytokines, vaccines or transgenes in new contexts that are typically not found in nature. The approach of DNA shuffling is a test-tube process that takes advantage of recombination to generate libraries of chimeric genes, which can then be screened to identify the encoded proteins ...
R G, Whalen +3 more
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RNA editing meets DNA shuffling
Nature, 2000The immune system can tailor its production of antibodies to the type of infection. Three sequential DNA-shuffling processes underlie this ability. The same protein is responsible for the last two of the processes, and oddly it is an RNA-editing rather than a DNA-recombining enzyme.
Ming Tian, Frederick W. Alt
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Science, 2001
The core technology behind directed molecular evolution is the polymerase chain reaction, the "molecular photocopier" that9s used to amplify small amounts of DNA. But scientists at one biotech that is using the technique to produce vaccines have given it a special tweak to produce what they call "DNA shuffling."
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The core technology behind directed molecular evolution is the polymerase chain reaction, the "molecular photocopier" that9s used to amplify small amounts of DNA. But scientists at one biotech that is using the technique to produce vaccines have given it a special tweak to produce what they call "DNA shuffling."
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