Results 201 to 210 of about 17,272 (247)

Development of an Effective Multiepitope Vaccine Against Infectious Laryngotracheitis Virus Based on Envelope Glycoproteins by Immunoinformatics Approaches. [PDF]

Arch Razi Inst
Parisa J, Maryam M, Narjes NG, Behrouz E, Asghar A.   +4 more
europepmc   +1 more source

Adaptive laboratory evolution and transcriptomic profiling reveal carbon-nitrogen metabolic reprogramming enabling aerobic co-fermentation of glucose and xylose in Saccharomyces cerevisiae. [PDF]

PLoS One
López-deÁvila LM, Monsalve-Fonnegra ZI, Rodríguez-Cabal HA.   +2 more
europepmc   +1 more source

Some of the next articles are maybe not open access.

Related searches:

Artificial evolution by DNA shuffling

Trends in Biotechnology, 1998
Improvement of enzymes is one of the important objectives of biotechnology. In vitro evolution of enzymes using DNA shuffling involves the assembly of two or more DNA segments into a full-length gene by homologous, or site-specific, recombination. Before the assembly, the segments are often subjected to random mutagenesis by error-prone PCR, random ...
Shigeaki Harayama
exaly   +3 more sources

The analysis of DNA shuffling by nMDS

2011 IEEE International Conference on Bioinformatics and Biomedicine Workshops (BIBMW), 2011
DNA shuffling is widely used for optimizing complex properties contained within DNA and proteins. However, success rate of it is deeply dependent upon which pair of DNAs is employed for DNA shuffling. In this paper, we have used non-metric multidimensional scaling (nMDS) to select best pair of DNAs for it.
Y-H Taguchi
exaly   +2 more sources

Rapid evolution of a protein in vitro by DNA shuffling

Nature, 1994
DNA shuffling is a method for in vitro homologous recombination of pools of selected mutant genes by random fragmentation and polymerase chain reaction (PCR) reassembly. Computer simulations called genetic algorithms have demonstrated the importance of iterative homologous recombination for sequence evolution.
Willem P C Stemmer
exaly   +3 more sources

DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production

Methods in Molecular Biology, 2013
DNA family shuffling is a powerful method of directed evolution applied for the generation of novel enzymes with the aim of improving their existing features or creating completely new enzyme properties. This method of evolution in vitro requires parental sequences containing a high level of sequence similarity, such as is found in family members of ...
Nedeljka Rosic
exaly   +5 more sources

ShuffleAnalyzer: A Comprehensive Tool to Visualize DNA Shuffling

ACS Synthetic Biology
DNA shuffling is a powerful technique for generating synthetic DNA via recombination of homologous parental sequences. Resulting chimeras are often incorporated into complex libraries for functionality screenings that identify novel variants with improved characteristics.
Franz Schweiggert, Patrick Most, Jörg Schweiggert   +2 more
exaly   +3 more sources

Applications of DNA shuffling to pharmaceuticals and vaccines

Current Opinion in Biotechnology, 1997
DNA shuffling is a practical process for directed molecular evolution which uses recombination to dramatically accelerate the rate at which one can evolve genes. Single and multigene traits that require many mutations for improved phenotypes can be evolved rapidly. DNA shuffling technology has been significantly enhanced in the past year, extending its
P A, Patten, R J, Howard, W P, Stemmer
openaire   +2 more sources