Results 151 to 160 of about 9,767 (189)
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Human Type I DNA Topoisomerase: Regulation of Expression and Function
1992The replication of DNA requires two fundamental steps, namely an unwinding of the parental DNA double helix and the utilization of the emerging single strand sequences as templates for the synthesis of progeny DNA strands. The unwinding of the double helix is catalyzed by DNA helicases breaking the hydrogen bonds between complementary DNA strands at ...
R. Knippers +4 more
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Biochemical and Biophysical Research Communications, 1983
2-6 dimethyl-9-hydroxyellipticinium inhibited the relaxation of supercoiled DNA by the type I topoisomerase of T. cruzi. Since DNA relaxed in the presence of an intercalating drug prior to electrophoresis became supercoiled when the ligand was removed, we analysed the topoisomerisation in gels containing another ligand, chloroquine.
S, Douc-Rasy, A, Kayser, G, Riou
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2-6 dimethyl-9-hydroxyellipticinium inhibited the relaxation of supercoiled DNA by the type I topoisomerase of T. cruzi. Since DNA relaxed in the presence of an intercalating drug prior to electrophoresis became supercoiled when the ligand was removed, we analysed the topoisomerisation in gels containing another ligand, chloroquine.
S, Douc-Rasy, A, Kayser, G, Riou
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Bacillus anthracis pXO1 virulence plasmid encodes a type 1 DNA topoisomerase
Molecular Microbiology, 1994SummaryThe virulence plasmid pXO1 is responsible for toxin production in Bacillus anthracis. A DNA fragment from pXO1 was isolated and was shown, by sequence analysis, to contain part of a type 1 DNA topoisomerase gene. Attempts to clone the entire wild‐type gene, designated topX, in Escherichia coli, were unsuccessful.
A, Fouet, J C, Sirard, M, Mock
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2000
The mitochondrial type I DNA topoisomerase (mt-topo I) serves an important function in the mitochondrion by relaxing mtDNA supercoils to allow for replication of the mitochondrial genome as well as for gene expression. The mt-topo I's role in essential processes, such as replication and transcription, makes it an ideal candidate as a target for ...
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The mitochondrial type I DNA topoisomerase (mt-topo I) serves an important function in the mitochondrion by relaxing mtDNA supercoils to allow for replication of the mitochondrial genome as well as for gene expression. The mt-topo I's role in essential processes, such as replication and transcription, makes it an ideal candidate as a target for ...
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Escherichia coli DNA topoisomerase III: purification and characterization of a new type I enzyme
Biochemistry, 1984A new topoisomerase capable of relaxing negatively supercoiled DNA in Escherichia coli has been identified during chromatography on novobiocin-Sepharose. A simple and reproducible purification procedure is described to obtain this enzyme, called topoisomerase III (topo III), in a homogeneous form.
K S, Srivenugopal +2 more
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In vitro analysis of a type I DNA topoisomerase activity from cultured tobacco cells
Plant Molecular Biology, 1992The role of DNA topoisomerases in plant cell metabolism is currently under investigation in our laboratory. Using a purified type I topoisomerase from cultured tobacco, we have carried out a biochemical characterization of enzymatic behavior. The enzyme relaxes negatively supercoiled DNA in the presence of MgCl2, and to a lesser extent in the presence ...
A D, Cole +3 more
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Conserved regulatory elements in the type I DNA topoisomerase gene promoters of mouse and man
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1994The gene for mammalian type I DNA topoisomerase is constitutively expressed, but also regulated by a number of external stimuli. We compared the nucleotide sequences of the human and the mouse topoisomerase I gene promoters because promoter elements, essential for basic as well as regulated gene expression, should be conserved during evolution.
B, Baumgärtner +4 more
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Future Medicinal Chemistry, 2011
Many anticancer drugs reduce the integrity of DNA, forming strand breaks. This can cause mutations and cancer or cell death if the lesions are not repaired. Interestingly, DNA repair-deficient cancer cells (e.g., those with BRCA1/2 mutations) have been shown to exhibit increased sensitivity to chemotherapy.
Józefa, Węsierska-Gądek +1 more
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Many anticancer drugs reduce the integrity of DNA, forming strand breaks. This can cause mutations and cancer or cell death if the lesions are not repaired. Interestingly, DNA repair-deficient cancer cells (e.g., those with BRCA1/2 mutations) have been shown to exhibit increased sensitivity to chemotherapy.
Józefa, Węsierska-Gądek +1 more
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Russian Journal of Bioorganic Chemistry, 2003
Interaction of the DNA type I topoisomerases from the murine and human placenta cells with nonspecific oligonucleotides was analyzed. The contributions of strong and week nonspecific electrostatic, van der Waals's, and hydrophobic interactions, and hydrogen bonding of the enzymes to the complex formation with the single- and double-stranded DNAs were ...
D V, Bugreev +3 more
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Interaction of the DNA type I topoisomerases from the murine and human placenta cells with nonspecific oligonucleotides was analyzed. The contributions of strong and week nonspecific electrostatic, van der Waals's, and hydrophobic interactions, and hydrogen bonding of the enzymes to the complex formation with the single- and double-stranded DNAs were ...
D V, Bugreev +3 more
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Cancer Research, 2010
Abstract Developing small molecule inhibitors in anti-cancer drug research is an integral part of clinical oncology. Gefitinib (Iressa) is a promising small molecule inhibitor of epidermal growth factor receptor (EGFR) tyrosine kinase. It was shown to have the potential to improve the clinical effectiveness of EGFR-targeted therapies and
Zeki Topcu, Isa Unlukurt, Sevil Zencir
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Abstract Developing small molecule inhibitors in anti-cancer drug research is an integral part of clinical oncology. Gefitinib (Iressa) is a promising small molecule inhibitor of epidermal growth factor receptor (EGFR) tyrosine kinase. It was shown to have the potential to improve the clinical effectiveness of EGFR-targeted therapies and
Zeki Topcu, Isa Unlukurt, Sevil Zencir
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