Results 221 to 230 of about 17,370 (252)

DNA relaxation mediated by Ustilago maydis type I topoisomerase; modulation by chromatin associated proteins

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1993
Ustilago maydis topoisomerase I relaxes superhelical DNA in the absence of any co-factors. The reaction reaches a defined end-point proportional to the amount of enzyme added and an analysis of the reaction by Hill plot transformation indicates that at least two molecules of topoisomerase must interact with the DNA to catalyze relaxation.
M M, Thiyagarajan, H, Kotani, W K, Holloman, E B, Kmiec   +3 more
openaire   +2 more sources

Mechanistic Characterization of the Mitochondrial Type I DNA Topoisomerase and a Study of Genes Containing Type I DNA Topoisomerase-Related Domains

2000
The mitochondrial type I DNA topoisomerase (mt-topo I) serves an important function in the mitochondrion by relaxing mtDNA supercoils to allow for replication of the mitochondrial genome as well as for gene expression. The mt-topo I's role in essential processes, such as replication and transcription, makes it an ideal candidate as a target for ...
openaire   +1 more source

Therapeutic Intervention by the Simultaneous Inhibition of DNA Repair and Type I or Type II DNA Topoisomerases: One Strategy, Many Outcomes

Future Medicinal Chemistry, 2011
Many anticancer drugs reduce the integrity of DNA, forming strand breaks. This can cause mutations and cancer or cell death if the lesions are not repaired. Interestingly, DNA repair-deficient cancer cells (e.g., those with BRCA1/2 mutations) have been shown to exhibit increased sensitivity to chemotherapy.
Józefa, Węsierska-Gądek, Andrzej, Składanowski   +1 more
openaire   +2 more sources

Effects of bifunctional netropsin-related minor groove-binding ligands on mammalian type I DNA topoisomerase

Biochemical Pharmacology, 1997
We investigated the effects of compounds with two covalently linked netropsin moieties (bis-netropsin) on the function of mammalian type I DNA topoisomerase (topo I) in vitro. We initiated these studies because earlier studies had shown that certain bis-netropsins possess a several-fold higher antitumor and antiviral activity than netropsin.
Z, Wang, C, Zimmer, J W, Lown, R, Knippers   +3 more
openaire   +2 more sources

Studies on Type I and Type II DNA Topoisomerases From Mammalian Mitochondria

1993
Mammalian mitochondria contain a unique DNA topoisomerase I with characteristics of both nuclear and bacterial type I DNA topoisomerases. This enzyme, purified from bovine liver, requires cations as cofactors and possesses optimal activity at pH 7.0, 37°C and either 150 mM K+ or Na+ ions.
openaire   +1 more source

Partial purification and characterization of type I DNA topoisomerase from Bacillus stearothermophilus.

Journal of biochemistry, 1985
Type I DNA topoisomerase was partially purified from Bacillus stearothermophilus by ammonium sulfate precipitation and column chromatographies on phosphocellulose, DEAE-cellulose and heparin-agarose. On heparin-agarose chromatography, topoisomerase I activity was separated into three fractions (designated Fractions A, B, and C).
N, Yamamoto, K, Shishido, H, Ogawa, T, Sekiguchi, Y, Nosoh   +4 more
openaire   +1 more source

Circulating tumor DNA in advanced solid tumors: Clinical relevance and future directions

Ca-A Cancer Journal for Clinicians, 2021
Michael L Cheng, Glenn J Hanna, Heather A Parsons   +2 more
exaly  

Inhibitory activities of benzoaxol derivatives on mammalian type I DNA topoisomerase

2019
Federat European Biochem ...
Soyer, Z., Kocadag, E., Pabuccuoglu, V., Telefoncu, A., Topcu, Z.   +4 more
openaire   +1 more source

Cancer epigenetics in clinical practice

Ca-A Cancer Journal for Clinicians, 2023
Veronica Davalos, Manel Esteller
exaly  

Structure of Eukaryotic Type I DNA Topoisomerase

1994
T, Hsieh, M P, Lee, S D, Brown
openaire   +2 more sources