Results 111 to 120 of about 2,219 (132)
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The Role of DnaA-DnaA Box Interaction in Initiation of Chromosomal Replication in Bacillus subtilis
1992In bacteria, an autonomously replicating sequence (ars) was first isolated from the replication origin region of the Escherichia coli chromosome (Yasuda and Hirota, 1977) and was defined as oriC (origin of the chromosomal replication). Genetic and biochemical studies in E.
N. Ogasawara, S. Moriya, H. Yoshikawa
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Biochimica et Biophysica Acta (BBA) - General Subjects, 2017
DnaA protein is the initiator of genomic DNA replication in prokaryotes. It binds to specific DNA sequences in the origin of DNA replication and unwinds small AT-rich sequences downstream for the assembly of the replisome. The mechanism of activation of DnaA that enables it to bind and organize the origin DNA and leads to replication initiation remains
Meera J, Patel +4 more
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DnaA protein is the initiator of genomic DNA replication in prokaryotes. It binds to specific DNA sequences in the origin of DNA replication and unwinds small AT-rich sequences downstream for the assembly of the replisome. The mechanism of activation of DnaA that enables it to bind and organize the origin DNA and leads to replication initiation remains
Meera J, Patel +4 more
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Specific Binding of DnaA Protein to a DnaA Box in the GuaB Gene of Escherichia Coli K12
European Journal of Biochemistry, 1996Expression of the guaBA operon of Escherichia coli is regulated by the DNA replication‐initiating protein, DnaA. Two DnaA boxes, which are potential binding sites for DnaA, are present in the gua operon. One box (with 819 match to the DnaA box consensus sequence) is at the gua promoter; the other box, which has a consensus sequence, is on the non ...
F, Tesfa-Selase, W T, Drabble
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Gene, 1988
Genes clockwise of oriC, the Escherichia coli replication origin (oriC-mioC-asnC), show anticlockwise transcription. The intergenic region between mioC and asnC contains both a terminator and a consensus DnaA-protein-binding site (dnaA box). We analysed termination in this region using galK expression to monitor for transcription. About 50% of the asnC
C, Schaefer, W, Messer
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Genes clockwise of oriC, the Escherichia coli replication origin (oriC-mioC-asnC), show anticlockwise transcription. The intergenic region between mioC and asnC contains both a terminator and a consensus DnaA-protein-binding site (dnaA box). We analysed termination in this region using galK expression to monitor for transcription. About 50% of the asnC
C, Schaefer, W, Messer
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Replication properties of mini-Rts1 derivatives deleted for DnaA boxes in the replication origin
Plasmid, 1989Mini-Rts1 was found to be unable to replicate in a dnaA-null mutant. However, a mini-Rts1 derivative lacking entire tandem DnaA boxes in the replication origin retained the replication ability in a dnaA+ host although its copy number was about half that of the mini-Rts1 having complete DnaA boxes. Mini-Rts1cop1 that contains a high copy number mutation
Y, Itoh, Y, Terawaki
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Journal of Molecular Biology, 2001
The DnaA protein is essential for initiation of DNA replication in a wide variety of bacterial and plasmid replicons. The replication origin in these replicons invariably contains specific binding sites for the protein, called DnaA boxes. Plasmid P1 contains a set of DnaA boxes at each end of its origin but can function with either one of the sets ...
Dhruba K Chattoraj, Kyusung Park
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The DnaA protein is essential for initiation of DNA replication in a wide variety of bacterial and plasmid replicons. The replication origin in these replicons invariably contains specific binding sites for the protein, called DnaA boxes. Plasmid P1 contains a set of DnaA boxes at each end of its origin but can function with either one of the sets ...
Dhruba K Chattoraj, Kyusung Park
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Journal of Structural Biology, 2012
In complex with ATP, but not ADP, DnaA protein multimers unwind a specific region of duplex DNA within the chromosomal replication origin, oriC, triggering a series of reactions that result in initiation of DNA replication. Following replication initiation, ATP hydrolysis, which is coupled to DNA replication, results in the generation of initiation ...
Tsutomu Katayama, Shogo Ozaki
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In complex with ATP, but not ADP, DnaA protein multimers unwind a specific region of duplex DNA within the chromosomal replication origin, oriC, triggering a series of reactions that result in initiation of DNA replication. Following replication initiation, ATP hydrolysis, which is coupled to DNA replication, results in the generation of initiation ...
Tsutomu Katayama, Shogo Ozaki
exaly +3 more sources
The New biologist, 1991
The dnaA gene of Escherichia coli specifies a product that is a key element in the initiation of DNA replication. Expression of dnaA occurs from two promoters, 1P and 2P, which flank a DnaA protein binding site (DnaA box). In this paper we describe the effects of DnaA box mutations on transcription from the two promoters, measured with the use of dnaA ...
P, Polaczek, A, Wright
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The dnaA gene of Escherichia coli specifies a product that is a key element in the initiation of DNA replication. Expression of dnaA occurs from two promoters, 1P and 2P, which flank a DnaA protein binding site (DnaA box). In this paper we describe the effects of DnaA box mutations on transcription from the two promoters, measured with the use of dnaA ...
P, Polaczek, A, Wright
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A comprehensive set of DnaA‐box mutations in the replication origin, oriC, of Escherichia coli
Molecular Microbiology, 1996We probed the complex between the replication origin, oriC, and the initiator protein DnaA using different types of mutations in the five binding sites for DnaA, DnaA boxes R1–R4 and M: (i) point mutations in individual DnaA boxes and combinations of them; (ii) replacement of the DnaA boxes by a scrambled 9 bp non‐box motif; (iii) positional exchange;
U, Langer +4 more
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