SMC ensures efficient chromosome replication and oriC positioning during Streptomyces spore germination [PDF]
Scientific ReportsBacterial chromosomes are organized by condensins, such as Structural Maintenance of Chromosomes (SMC) proteins. In Streptomyces, a genus of sporulating bacteria, SMC proteins align chromosomal arms and promote efficient compaction of chromosomal DNA ...
Katarzyna Pawlikiewicz +7 more
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Organizational readiness for implementing clinical decision support tools in chronic pain care: a mixed methods evaluation [PDF]
Frontiers in Health ServicesIntroductionClinical decision support (CDS) tools have the potential to enhance treatment outcomes in chronic pain care, yet their successful implementation depends on the readiness of both providers and clinical staff.
Miranda J. Reid +9 more
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Measuring Organizational Readiness for Implementing Change in Primary Care Facilities in Rural Bushbuckridge, South Africa [PDF]
International Journal of Health Policy and Management, 2022 Meaningful gains in health outcomes require successful implementation of evidence-based interventions. Organizations such as health facilities must be ready to implement efficacious interventions, but tools to measure organizational readiness have rarely
Hannah H. Leslie +6 more
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Frontiers in Microbiology, 2021 The structure and function of bacterial chromosomes are dynamically regulated by a wide variety of nucleoid-associated proteins (NAPs) and DNA superstructures, such as DNA supercoiling.
Kazutoshi Kasho +5 more
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Development and host compatibility of plasmids for two important ruminant pathogens, Mycoplasma bovis and Mycoplasma agalactiae. [PDF]
PLoS ONE, 2015 Mycoplasma bovis is a cause of pneumonia, mastitis, arthritis and otitis media in cattle throughout the world. However, despite its clinical significance, there is a paucity of tools to genetically manipulate it, impeding our capacity to further explore ...
Shukriti Sharma +5 more
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In vitro amplification of whole large plasmids via transposon-mediated oriC insertion
BioTechniques, 2021 DNA amplification is a fundamental technique in molecular biology. The replication cycle reaction is a new method for amplification of large circular DNA having oriC sequences, which is a replication initiation site of the Escherichia coli chromosome. We
Seia Nara, Masayuki Su'etsugu
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Scientific Reports, 2023 Ion channels are vital components of filamentous fungi signaling in communication with their environment. We exploited the ability of the apical region of growing sporangiophores of Phycomyces blakesleeanus to form membrane-enveloped cytoplasmic droplets
Katarina S. Stevanović +5 more
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Chromosomal Position of Ribosomal Protein Genes Affects Long-Term Evolution of Vibrio cholerae
mBio, 2023 It is unclear how gene order within the chromosome influences genome evolution. Bacteria cluster transcription and translation genes close to the replication origin (oriC).
Leticia Larotonda +8 more
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AdpA, key regulator for morphological differentiation regulates bacterial chromosome replication [PDF]
Open Biology, 2012 AdpA, one of the most pleiotropic transcription regulators in bacteria, controls expression of several dozen genes during Streptomyces differentiation.
Marcin Wolański +2 more
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Evolutionary Changes in DnaA-Dependent Chromosomal Replication in Cyanobacteria
Frontiers in Microbiology, 2020 Replication of the circular bacterial chromosome is initiated at a unique origin (oriC) in a DnaA-dependent manner in which replication proceeds bidirectionally from oriC to ter. The nucleotide compositions of most bacteria differ between the leading and
Ryudo Ohbayashi +11 more
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