Results 11 to 20 of about 7,181 (295)

In vitro amplification of whole large plasmids via transposon-mediated oriC insertion

BioTechniques, 2021
DNA amplification is a fundamental technique in molecular biology. The replication cycle reaction is a new method for amplification of large circular DNA having oriC sequences, which is a replication initiation site of the Escherichia coli chromosome. We
Seia Nara, Masayuki Su'etsugu
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ORIC central region calculations [PDF]

Proceedings Particle Accelerator Conference, 2002
The central region for the K=100 Oak Ridge Isochronous Cyclotron, ORIC, will be modified to provide better orbit centering, focusing of orbits in the axial direction, and phase selection, in order to improve extraction efficiency, and reduce radioactive activation of cyclotron components. The central region is specifically designed for the acceleration
Bailey, J. D., Dowling, D. T., Lane, S. N., Mosko, S. W., Olsen, D. K., Tatum, B. A.   +5 more
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Mutations in the CCGTTCACA DnaA Box of Mycobacterium tuberculosis oriC That Abolish Replication of oriC Plasmids Are Tolerated on the Chromosome [PDF]

Journal of Bacteriology, 2002
ABSTRACT The origin of replication ( oriC ) region in some clinical strains of Mycobacterium tuberculosis is a hot spot for IS 6110 elements. To understand how clinical strains with insertions in oriC can replicate their DNA, we ...
Jaroslaw, Dziadek, Malini, Rajagopalan, Tanya, Parish, Natalia, Kurepina, Rebecca, Greendyke, Barry N, Kreiswirth, Murty V V S, Madiraju   +6 more
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Development of oriC-plasmids for use in Mycoplasma hyorhinis

Scientific Reports, 2017
AbstractMycoplasma hyorhinis (M. hyorhinis) is an opportunistic pig pathogen, belonging to the class Mollicutes. It causes polyserositis, arthritis and cancers in vitro, increasing attention of the researchers. Currently, there is no available genetic tool to manipulate its genome.
Ishag, Hassan Z. A., Xiong, Qiyan, Liu, Maojun, Feng, Zhixin, Shao, Guoqing   +4 more
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Analysis of transcription at the oriC locus in Mycobacterium tuberculosis

Microbiological Research, 2011
Details of the mechanism of DNA replication in the slow growing pathogen Mycobacterium tuberculosis (M. tb) are unknown. The dnaA and dnaN gene products are essential for chromosome replication and growth of a bacterium. Here we analyzed the transcriptional activity at the oriC locus in M. tb that includes dnaA, dnaN and recF.
Chauhan, Santosh, Tyagi, Jaya Sivaswami
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Assessing the reliability and validity of the Danish version of Organizational Readiness for Implementing Change (ORIC)

Implementation Science, 2018
Background Organizational change initiatives in health care frequently achieve only partial implementation success. Understanding an organizational readiness for change (ORC) may be a way to develop more effective and efficient change strategies. Denmark,
Marie Höjriis Storkholm, Pamela Mazzocato, Mesfin Kassaye Tessma, Carl Savage   +3 more
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Development and host compatibility of plasmids for two important ruminant pathogens, Mycoplasma bovis and Mycoplasma agalactiae. [PDF]

PLoS ONE, 2015
Mycoplasma bovis is a cause of pneumonia, mastitis, arthritis and otitis media in cattle throughout the world. However, despite its clinical significance, there is a paucity of tools to genetically manipulate it, impeding our capacity to further explore ...
Shukriti Sharma, Chistine Citti, Eveline Sagné, Marc S Marenda, Philip F Markham, Glenn F Browning   +5 more
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ATP modulation of osmotically activated anionic current in the membrane of Phycomyces blakesleeanus sporangiophore

Scientific Reports, 2023
Ion channels are vital components of filamentous fungi signaling in communication with their environment. We exploited the ability of the apical region of growing sporangiophores of Phycomyces blakesleeanus to form membrane-enveloped cytoplasmic droplets
Katarina S. Stevanović, Bogdana Čepkenović, Strahinja Križak, Tanja Pajić, Nataša V. Todorović, Miroslav Ž. Živić   +5 more
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Chromosomal Position of Ribosomal Protein Genes Affects Long-Term Evolution of Vibrio cholerae

mBio, 2023
It is unclear how gene order within the chromosome influences genome evolution. Bacteria cluster transcription and translation genes close to the replication origin (oriC).
Leticia Larotonda, Damien Mornico, Varun Khanna, Joaquín Bernal-Bayard, Jean-Marc Ghigo, Marie-Eve Val, Diego Comerci, Didier Mazel, Alfonso Soler-Bistué   +8 more
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Characterization of the oriC region of Mycobacterium smegmatis [PDF]

Journal of Bacteriology, 1997
A 3.5-kb DNA fragment containing the dnaA region of Mycobacterium smegmatis has been hypothesized to be the chromosomal origin of replication or oriC (M. Rajagopalan et al., J. Bacteriol. 177:6527-6535, 1995). This region included the rpmH gene, the dnaA gene, and a major portion of the dnaN gene as well as the rpmH-dnaA and dnaA-dnaN intergenic ...
M H, Qin, M V, Madiraju, S, Zachariah, M, Rajagopalan   +3 more
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