Results 161 to 170 of about 55,772 (184)

Evaluation of dot-ELISA for serological diagnosis of amebiasis

Journal of Infection and Chemotherapy, 2003
We improved the dot enzyme-linked immunosorbent assay (dot-ELISA) reported by Itoh and Sato, and assessed the usefulness of this test for the diagnosis of amebiasis. The sensitivity of dot-ELISA was compared with that of plate ELISA, the indirect hemagglutination test (IHA), and the indirect fluorescent antibody test (IFA) for the diagnosis of ...
Hisashi, Yamaura, Kunioki, Araki, Ken, Kikuchi, Ichiro, Itoda, Kyoichi, Totsuka, Takatoshi, Kobayakawa   +5 more
openaire   +2 more sources

Diagnosis of Echinococcosis (Hydatidosis) Using Dot-ELISA

The Egyptian Journal of Hospital Medicine, 2016
Background:cystic echinococcosis (CE) is a complex, chronic and neglected disease caused by the larval stage of Echinococcus granulosus. The effects of this neglection have a powerful impact in remote rural areas whose population has no chances of being diagnosed and treated correctly without leaving their works and travelling long distances, sometimes
A. M. El-Ameer, M. M. Ramadan, I. R. Shalash   +2 more
openaire   +1 more source

Recent applications of the Dot-ELISA in immunoparasitology

Veterinary Parasitology, 1988
The dot enzyme-linked immunosorbent assay (Dot-ELISA) is a highly versatile solid-phase immunoassay for antibody or antigen detection. The assay uses minute amounts of reagent dotted onto solid surfaces such as nitrocellulose and other paper membranes which avidly bind proteins. After incubation with antigen-specific antibody and enzyme-conjugated anti-
openaire   +2 more sources

Simplification and Standardization of Dot-ELISA for Human Schistosomiasis mansoni

The Journal of Parasitology, 1987
Dot-ELISA, a technique that shares the same principles as the enzyme immunoassay, is useful for detection of anti-Schistosoma mansoni antibodies in the sera of patients with Schistosoma mansoni infections. The antigens were fixed to the nitrocellulose strips, blocked with 1% bovine serum albumin in 0.05% Tween 20.
F N, Boctor, M J, Stek, J B, Peter, R, Kamal   +3 more
openaire   +2 more sources

Dot-ELISA in diagnosis of schistosomiasis.

Journal of the Egyptian Society of Parasitology, 1989
One microliter of S. mansoni egg antigen was dotted directly on the nitrocellulose paper sheet acting as the adsorbent surface (9 dots/paper). The sera of 25 Egyptian patients and 15 healthy persons (2 microliters of each) were dotted over the antigen dots, then 2 ml of each of the blocking, washing, HRP-conjugated IgG and DAB adding procedures, were ...
M A, Madwar, M M, Hassan
openaire   +1 more source

[Dot-ELISA in the diagnosis of neurocysticercosis].

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 1991
The dot-enzyme-linked immunosorbent assay was used to detect Cysticercus cellulosae antibodies in sera of patients with neurocysticercosis. Among 108 confirmed cases of neurocysticercosis 81.6-96.1% showed positive reactions. Two out of 54 normal control sera reacted at a serum dilution of 1:20, but none at a 1:40 (range 40-640).
H J, Jiang, L X, Yang, S H, Pang, T, Sun, S P, Xie, J M, Meng, G Z, Wen, G Z, Wang   +7 more
openaire   +1 more source

An improved dot ELISA to detect fowl adenovirus type-1 antigen

Comparative Immunology, Microbiology and Infectious Diseases, 1993
An improved dot immunobinding assay to detect fowl adenovirus type-1 is described. The method consists of spotting of antigen on nitrocellulose membrane sheet, blocking with either 5% acetic acid or with 5% defatted milk powder and fixation of either antigen or antigen-antibody complex, with either 50% methanol or 0.25% glutaraldehyde or 0.2% tannic ...
N K, Maiti, S S, Saini, R, Singh, M S, Oberoi, S N, Sharma   +4 more
openaire   +2 more sources

Rapid detection of potato viruses by dot-ELISA

Potato Research, 1988
The dot-ELISA, an immunosorbent assay on nitrocellulose (NC) membranes, was applied to potato viruses. The sensitivity of the test, as determined by visual assessment of the colour reactions in serial dilutions, allowed a reliable detection of the potato viruses M, S, X, Y, A and of potato leafroll virus in leaves of non-potato hosts and potato plants.
openaire   +1 more source

Detection of leprosy antigenuria through DOT-ELISA.

Indian journal of leprosy, 1989
Fifty-five samples of urine from different grades of leprosy patients and normal persons were processed for detection of PGL-1 antigen through DOT-ELISA on nitrocellulose paper strips using anti-human IgG horse raddish peroxidaseconjugate. About 66.6% of the paucibacillary and 100% of the multibacillary leprosy cases were detectable through this ...
N B, Singh, A, Choudhary
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Detection of Artichoke Latent Virus by an Indirect Dot‐ELISA

Journal of Phytopathology, 1991
AbstractAn indirect Dot‐ELISA was compared with DAS‐ELISA for detecting Artichoke Latent Virus (ALV) both in purified preparations and in crude sap from “Spinoso sardo” artichoke leaves.Antigen diluted samples (1 μl) were first spotted on nitrocellulose (NC) and polyvinylidene difluoride (PVDF) membranes. After blocking, the membranes were incubated in
M. Cugusi, A. Foddai, G. Idini
openaire   +1 more source