Results 41 to 50 of about 98,431 (180)

Comparison of two laboratory-developed PCR methods for the diagnosis of Pulmonary Tuberculosis in Brazilian patients with and without HIV infection

open access: yesBMC Pulmonary Medicine, 2011
Background Direct smear examination with Ziehl-Neelsen (ZN) staining for the diagnosis of pulmonary tuberculosis (PTB) is cheap and easy to use, but its low sensitivity is a major drawback, particularly in HIV seropositive patients.
Netto Antonio   +9 more
doaj   +1 more source

Immunoblotting and dot blotting

open access: yesJournal of Immunological Methods, 1989
A variety of methods has been available for many years for the analytical separation of mixtures of proteins into their component parts by electrophoresis in a gel, usually agarose or polyacrylamide. Popular techniques are: zonal electrophoresis in agarose gel or on cellulose acetate membranes; discontinuous electrophoresis in polyacrylamide gel (PAGE);
openaire   +2 more sources

Identification of BmhGH-like protein by Western blot and Dot blot. [PDF]

open access: yes, 2014
Western blot. M: LMW protein marker; 1: total protein sample; 2: absorption peak #1; 3: absorption peak #2; 4: absorption peak #3; 5: absorption peak #4. B. Dot blot.
Zuoming Nie (50318)   +8 more
core   +1 more source

Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation [PDF]

open access: yes, 1992
Aim: To determine the relative diagnostic performance of non-isotopic in situ hybridisation (NISH) and a dot-blot assay for detecting human papillomavirus (HPV) on exfoliated cervical cells; and to correlate the results with cytopathological assessment ...
TRONCONE, GIANCARLO   +13 more
core   +1 more source

Detection and characterization of purified antigenic proteins from culture filtrate of Mycobacterium bovis strain AN5

open access: yesIranian Journal of Microbiology, 2020
Background and Objectives: Bovine tuberculosis diagnosis is usually performed by various tests with specific limitations. Mycobacterium bovis culture filtrate contains antigenic proteins that could be used to improve the sensitivity of bovine ...
Malihe Honarmand Kashi   +3 more
doaj   +1 more source

Novel molecular diagnostic system of limbal stem cell deficiency based on MUC5AC transcript detection in corneal epithelium by PCR-reverse dot blot [PDF]

open access: yes, 2013
Purpose. To evaluate the sensitivity and specificity of a PCR-strip system based on reverse dot blot for detection of MUC5AC mRNA in corneal epithelium samples from patients with limbal stem cell deficiency (LSCD), and to determine the correlation with ...
Torras, Josep   +12 more
core   +1 more source

Affiblot: screeningové dot-blot zařízení pro selekci vhodných protilátek [PDF]

open access: yes, 2021
The key factor in the development of antibody-based assays is to find an antibody that has an appropriate affinity, high specificity, and low cross-reactivity.
Novotný, Jakub   +5 more
core   +1 more source

Quantification of matrix metalloproteinases MMP-8 and MMP-9 in gingival overgrowth

open access: yesSaudi Dental Journal, 2021
Background: Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in extracellular matrix remodeling of all body tissues, including oral tissues such as gingival tissue. Expression levels of MMPs are widely studied as important biomarkers for
Jennifer Orozco-Páez   +3 more
doaj   +1 more source

Southern dot blot analysis of selected SCOTS clones. [PDF]

open access: yes, 2014
Southern dot blot showing (I) SCOTS clones (each dot corresponds to one clone in Table 2) and (II) 0.1 µg (top) or 1 µg (bottom) of genomic S. agalactiae DNA as positive controls, hybridized to a digoxigenin (DIG)-labeled probe generated from RAW264.7 ...
Dildar Hussain Kalhoro (518943)   +6 more
core   +1 more source

Dot blot of all 38 defatted, buffer D extracted peanut samples (1μg/dot). [PDF]

open access: yes, 2016
(A) Dot blot layout. Samples were blotted with serum from an atopic (mold sensitive), non-food allergic patient with presumably no peanut-specific IgE (B) and a peanut allergic individual (C).
Soheila J. Maleki (2838623)   +2 more
core   +1 more source

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