Results 91 to 100 of about 2,513,493 (257)
, 2011 Shows the results of 3' RACE PCR using four different forward primers used in primary and nested PCRs for PKR1 (lanes 1–4) and three different forward primers combinations for PKR2 and PKR3 (lanes 5–7). The smaller fragment represents PKR2 and the larger
Stefan Rothenburg (66518) +4 more
core +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Tat-responsive region RNA of human immunodeficiency virus 1 can prevent activation of the double-stranded-RNA-activated protein kinase [PDF]
, 1990 Transcription from the human immunodeficiency virus type 1 promoter gives rise to short cytoplasmic transcripts of approximately 60 nucleotides as well as to longer mRNAs.
Rice, A. P. +7 more
core +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Activation of PKR by Bunyamwera virus is independent of the viral interferon antagonist NSs [PDF]
, 2003 Double-stranded RNA (dsRNA) is a by-product of viral RNA polymerase activity, and its recognition is one mechanism by which the innate immune system is activated.
Boyd, A. +25 more
core +1 more source
BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells
FEBS Open Bio, EarlyView.BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley +1 more source
FEBS Open Bio, EarlyView.Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada +7 more
wiley +1 more source
Double-Stranded RNA Is Detected by Immunofluorescence Analysis in RNA and DNA Virus Infections, Including Those by Negative-Stranded RNA Viruses. [PDF]
, 2015 Early biochemical studies of viral replication suggested that most viruses produce double-stranded RNA (dsRNA), which is essential for the induction of the host immune response.
Z Liang (7928936) +2 more
core +1 more source
, 2011 Shows the results of 3' RACE PCR using four different forward primers used in primary and nested PCRs for PKR1 (lanes 1–4) and three different forward primers combinations for PKR2 and PKR3 (lanes 5–7). The smaller fragment represents PKR2 and the larger
Stefan Rothenburg (66518) +4 more
core +1 more source
A small RNA response at DNA ends in Drosophila [PDF]
, 2012 Small RNAs have been implicated in numerous cellular processes, including effects on chromatin structure and the repression of transposons. We describe the generation of a small RNA response at DNA ends in Drosophila that is analogous to the recently ...
Förstemann, Klaus +2 more
core +1 more source