Results 61 to 70 of about 628,414 (263)

Low level sequence variant analysis of recombinant proteins: an optimized approach.

PLoS ONE, 2012
Sequence variants in recombinant biopharmaceuticals may have a relevant and unpredictable impact on clinical safety and efficacy. Hence, their sensitive analysis is important throughout bioprocess development.
Anne Zeck, Jörg Thomas Regula, Vincent Larraillet, Björn Mautz, Oliver Popp, Ulrich Göpfert, Frank Wiegeshoff, Ulrike E E Vollertsen, Ingo H Gorr, Hans Koll, Apollon Papadimitriou   +10 more
doaj   +1 more source

Amino Acid Sequences of Double-headed Proteinase Inhibitors from the Seeds ofCanavalia lineata

Bioscience, Biotechnology, and Biochemistry, 1994
The amino acids of two Bowman-Birk type proteinase inhibitors (CLTI-I and -II) from the seeds of Canavalia lineata were sequenced by a manual Edman degradation using the DABITC/PITC double coupling method after enzymatic digestions with Achromobacter lyticus lysyl endopeptidase, Staphylococcus aureus V8 protease, and chymotrypsin.
Terada, Shigeyuki, Fujimura, Satoshi, Kimoto, Eiji   +2 more
openaire   +2 more sources

ZW4864‐mediated inhibition of the β‐catenin/BCL9/BCL9L complex reveals therapeutic potential in bladder cancer

Molecular Oncology, EarlyView.
BCL9 and BCL9L drive bladder cancer progression by enhancing β‐catenin signaling, promoting proliferation, migration, invasion, and organoid growth. Genetic depletion of BCL9(L) suppresses malignant phenotypes, while pharmacological disruption of the β‐catenin/BCL9(L) complex with ZW4864 inhibits canonical Wnt signaling and tumor‐associated cellular ...
Roland Kotolloshi, Mandy Berndt‐Paetz, Eileen Lerner, Gregoire Najjar, Anca Azoitei, Krishna Pal Singh, Shailendra Kumar Gupta, Olaf Wolkenhauer, Cagatay Günes, Otmar Huber, Marc‐Oliver Grimm, Daniel Steinbach   +11 more
wiley   +1 more source

Different characteristics of 40 kinds of free amino acids and 5-amino levulinic acid between wines and sakes (Japanese rice wines)

Journal of Future Foods
Based on a systemic analysis for the global burden of disease study from 1990 to 2016, the Global Burden of Diseases (GBD) 2016 Alcohol Collaborators reported that alcohol use was a leading risk factor for global disease burden and caused substantial ...
Tohru Kimura
doaj   +1 more source

Targeting transcription factors associated with hemoglobinopathies: Lessons from successful interventions and implications for cancer

Molecular Oncology, EarlyView.
This review summarizes the transcription factors, repressive chromatin‐modifying complexes, and epigenetic mechanisms that control fetal hemoglobin repression. Notably, many regulators of γ‐globin silencing also function in transcriptional and epigenetic networks that drive cancer, highlighting opportunities to translate advances in hemoglobinopathy ...
Meigen Yu, Puspa Das, Xing Zhang, Xiaodong Cheng   +3 more
wiley   +1 more source

High gas barrier coating using non-toxic nanosheet dispersions for flexible food packaging film

Nature Communications, 2019
Food packaging industry is in search of non-toxic, stable, and recyclable coatings acting as gas-barriers. Here the authors synthesise transparent films meeting these requirements, based on high aspect ratio layered double hydroxide nanosheets obtained ...
Jingfang Yu, Kanittika Ruengkajorn, Dana-Georgiana Crivoi, Chunping Chen, Jean-Charles Buffet, Dermot O’Hare   +5 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysis and evaluation of nutritional value of Zhejiang miscellaneous meal protein resources

Siliao yanjiu
This experiment aimed to investigate the nutritional characteristics of major miscellaneous meal protein feed ingredients in Zhejiang Province. A total of 55 samples of rice bran meal, double-low rapeseed meal, and tea seed meal were collected from the ...
WANG Bin, DING Yanxin, LIN Xianjun, RUAN Xin, CHEN Kai, LIU Xia, CHEN Jie   +6 more
doaj  

Properties and Crystal Structure of the Cereibacter sphaeroides Photosynthetic Reaction Center with Double Amino Acid Substitution I(L177)H + F(M197)H. [PDF]

Membranes (Basel), 2023
Fufina TY, Selikhanov GK, Gabdulkhakov AG, Vasilieva LG.   +3 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source