Results 71 to 80 of about 935,473 (268)

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Quantifying protein residues in APIs: Bradford assay mechanism and limitations, outperformed by HILIC-MS/MS

Journal of Pharmaceutical Analysis
The molecular complexity of active pharmaceutical ingredients (APIs) has increased over recent decades, with production largely relying on microbial processes such as fermentation.
Yangrui Zhang, Yizhen Liu, Yangyang Chen, Chen Guo, Fengting Ou, Junhuan Lin, Hanmeng Guo, Tao Ke, Lushan Yu   +8 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Chemical Evolution and Biological Evaluation of Natural Products for Efficient Therapy of Acute Lung Injury

Advanced Science
Acute lung injury (ALI) is one of the most common complications in COVID‐19 and also a syndrome of acute respiratory failure with high mortality rates, but lacks effective therapeutic drugs.
Chengcheng Fan, Zeyi Zhang, Zhencheng Lai, Yanzi Yang, Jiaming Li, Lei Liu, Siyu Chen, Xueping Hu, Huajun Zhao, Sunliang Cui   +9 more
doaj   +1 more source

The autophagic regulation of rosiglitazone-promoted adipocyte browning

Frontiers in Pharmacology
Objective: Browning of white adipocytes is considered an efficient approach to combat obesity. Rosiglitazone induces the thermogenetic program of white adipocytes, but the underlying mechanisms remain elusive.Methods: Expression levels of browning and ...
Yue Li, Wanqing Zheng, Xinhang Li, Zhengwei Lue, Yun Liu, Jiaying Wu, Xiangnan Zhang, Xiangnan Zhang   +7 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

An inflammation-responsive therapeutic gel for precise microbiota modulation in colitis

Journal of Nanobiotechnology
Microbiome dysbiosis is a hallmark of inflammatory bowel disease (IBD), and its reprogramming represents a promising intervention strategy. Existing treatments are often limited by gastrointestinal instability and non-specific microbial toxicity, leading
Yanchen Liu, Yang Liu, Wenpeng Huang, Yazhou Liu, Jian Sun   +4 more
doaj   +1 more source

Deep Learning for Predicting Biomolecular Binding Sites of Proteins

Research
The rapid evolution of deep learning has markedly enhanced protein–biomolecule binding site prediction, offering insights essential for drug discovery, mutation analysis, and molecular biology.
Minjie Mou, Zhichao Zhang, Ziqi Pan, Feng Zhu   +3 more
doaj   +1 more source

Phosphoinositides and inositol phosphates as molecular glues

FEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry, Alexander J. Cutright, Mong Na Claire Loi, Jessica Martin, Joseph W. Gilligan, Jakub N. Kubina, Michael J. Arambula, Susanna Tanada Palmu, Daniella Zhou, Raymond D. Blind   +9 more
wiley   +1 more source

Accurate Identification of Protein Binding Sites for All Drug Modalities Using ALLSites

Advanced Science
Proteins interact with diverse molecular modalities, yet the incomplete identification of their binding sites has left the proteome‐wide druggability largely underexplored.
Minjie Mou, Mingkun Lu, Zhimeng Zhou, Yanlin Ren, Xinyuan Yu, Ziqi Pan, Yuan Zhou, Hao Yang, Lingyan Zheng, Shukai Gu, Yang Zhang, Wei Hu, Fengcheng Li, Haibin Dai, Feng Zhu   +14 more
doaj   +1 more source