Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Convective drying is one of the most commonly employed preservation techniques for food. However, the use of high temperatures and extended drying times often leads to a reduction in product quality and increased energy consumption.
Dominik Mierzwa, Grzegorz Musielak
doaj +1 more source
Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho +4 more
wiley +1 more source
Effect of supplementary cementitious materials on capillary sorption : relation with drying rate and testing time [PDF]
The water sorption phenomenon in a cementitious matrix is responsible for the ingress of several deleterious agents, and it is directly related to pore connectivity and pore volume.
Alderete, Natalia Mariel +2 more
core
Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source
Thin Layer Drying Characteristic of Morinda Citrifolia Fruit and Drying Effects on 6 – Methoxy-7-Hydoxy-Coumarin Content [PDF]
This study presents the thin layer drying characteristic of Morinda Citrifolia Linn fruit and drying effects on scopoletin content. The optimum mature stage of M.citrifolia fruit was found to be four months after bud flowering with high scopoletin ...
Sulaiman, Rabiha
core
Research on dynamics and drying time in microwave paper drying
Drying kinetics of paperboard with different grammages have been investigated in a laboratory microwave drier. The experiments were performed at four different values of specific microwave power between 440 W kg-1 and 1730 W kg-1. Experimental data of time varying paper moisture content were approximated with exponential Tomas&Skansi model and neural ...
Sander, Aleksandra +2 more
openaire +2 more sources
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Microwave Vacuum Drying Characteristics of Noni Fruits (Morinda Citrifolia L.) and Their Effects on Scopoletin Content [PDF]
The main objective of this study was to investigate microwave-vacuum drying characteristics of Morinda citrifolia fruit slices and drying effects on Scopoletin content.
Asr, Mina Habibi
core
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero +5 more
wiley +1 more source

