Results 101 to 110 of about 337,484 (235)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

An Efficient Method for Solving the Brusselator System

open access: yesWalailak Journal of Science and Technology, 2013
In this paper, a new efficient recurrence relation is constructed to solve a nonlinear Brusselator equation. The system, known as the reaction-diffusion Brusselator, arises in the modeling of certain diffusion processes.
Hossein AMINIKHAH, Ali JAMALIAN
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

open access: yesFEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui   +10 more
wiley   +1 more source

A finite element data assimilation method for the wave equation

open access: yes, 2018
We design a primal-dual stabilized finite element method for the numerical approximation of a data assimilation problem subject to the acoustic wave equation.
Burman, Erik   +2 more
core  

Dual Boundary Element Method for Instability Analysis of Cracked Plates

open access: yesComputer Modeling in Engineering & Sciences, 2005
This paper presents the dual boundary integral equations for the buckling analysis of the shear deformable cracked plates. The domain integrals which appear in this formulation are transferred to boundary integrals using the dual reciprocity method.
J. Purbolaksono, M. H. Aliabadi
openaire   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

A Comuputerized DRBEM model for generalized magneto-thermo-visco-elastic stress waves in functionally graded anisotropic thin film/substrate structures

open access: yesLatin American Journal of Solids and Structures
A numerical computer model, based on the dual reciprocity boundary element method (DRBEM) for studying the generalized magneto-thermo-visco-elastic stress waves in a rotating functionally graded anisotropic thin film/substrate structure under pulsed ...
Mohamed Abdelsabour Fahmy
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

On Duality, Symmetry and Symmetry lost in Solid Mechanics

open access: yes, 2012
International audienceThe paper recalls the concept of duality in Mathematics and extends it to Solid Mechanics. One important application of duality is to restore some symmetry between current fields and their adjoint ones.
Bui, Hui Duong
core   +1 more source

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