Results 161 to 170 of about 137,150 (318)

Dynamic analysis of the vehicle-structure interaction: a direct and efficient computer implementation

open access: yes
The simulation of the dynamic behavior of a structure subjected to sets of moving loads originated by vehicles whose structural behavior is also considered corresponds to a task not efficiently addressed by standard finite element packages.
Neves, Sérgio   +2 more
core  

Toward a general framework for dynamic road pricing

open access: yes, 2005
This paper develops a general framework for analysing and calculating dynamic road toll. The optimal network flow is first determined by solving an optimal control problem with statedependent responses such that the overall benefit of the network ...
Chow, A.
core  

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

open access: yesFEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Dynamics of Vehicle Loads on the Asphalt Pavement of European Roads Which Cross Lithuania

open access: yesThe Baltic Journal of Road and Bridge Engineering, 2007
The largest damage to the road pavements is caused by the heavy goods vehicles (HGV's). Due to large axle loads, which often assume a dynamic effect, the HGV's account for almost the total destructive impact on the road pavement, though they make about ...
Henrikas Sivilevicius   +1 more
doaj  

Time domain simulation of hydroelastic response of ships in large amplitude waves

open access: yes, 2006
The influence of non-linearities on wave-induced motions and loads has been the focus of many investigations in the past few years and continues to be an important issue.
Park, Jae-Hong
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

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