Results 261 to 270 of about 131,570 (298)

A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3)

2021
Continuous processing allows to maximize space-time yields and is implemented in many industrial branches. However, in manufacturing of value added compounds produced with microbial hosts, continuous processing is not state-of-the-art yet. This is because fluctuating productivity causes unwanted process deviations.
Julian, Kopp, Oliver, Spadiut
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On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains

Journal of Biotechnology, 2019
The CRISPR-Cas9 system is a new tool that has been extensively used for genome editing. The system is composed of a Cas9 endonuclease, which has the function of cleaving DNA at a specific site, and a guide RNA (gRNA), which contains the sequence of the cleavage site that is the target of editing.
Gabriela Pannunzio, Carmignotto, Adriano Rodrigues, Azzoni   +1 more
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Overexpression of a lethal methylase, M.TneDI, in E. coli BL21(DE3)

Biotechnology Letters, 2014
A pET-based vector pDH21 expressing the methylase, M.TneDI (recognizing CGCG) from Thermotoga was constructed, and transformed into E. coli BL21(DE3). Despite E. coli BL21(DE3) being McrBC positive, 30 transformants were isolated, which were suspected to be McrBC(-) mutants. The overexpression of M.TneDI was verified by SDS-PAGE analysis.
Hui, Xu, Dongmei, Han, Zhaohui, Xu
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Rapid label‐free quantitative analysis of the E. coli BL21(DE3) inner membrane proteome

PROTEOMICS, 2015
Biological membranes define cells and cellular compartments and are essential in regulating bidirectional flow of chemicals and signals. Characterizing their protein content therefore is required to determine their function, nevertheless, the comprehensive determination of membrane‐embedded sub‐proteomes remains challenging.
Malvina, Papanastasiou, Georgia, Orfanoudaki, Nikos, Kountourakis, Marina, Koukaki, Marios Frantzeskos, Sardis, Michalis, Aivaliotis, Konstantinos C, Tsolis, Spyridoula, Karamanou, Anastassios, Economou   +8 more
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Characterization of recombinant pectate lyase refolded from inclusion bodies generated in E. coli BL21(DE3)

Protein Expression and Purification, 2015
Pectate lyase (EC 4.2.2.2) gene from Bacillus subtilis RCK was cloned and expressed in Escherichia coli to maximize its production. In addition to soluble fraction, bioactive pectate lyase was also obtained from inclusion body aggregates by urea solubilization and refolding under in vitro conditions. Enzyme with specific activity ∼3194IU/mg and ∼1493IU/
Sandeep, Kumar, Kavish Kumar, Jain, Anupam, Singh, Amulya K, Panda, Ramesh Chander, Kuhad   +4 more
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CLONING AND EXPRESSION OFΒSUBUNIT GENE OF PHYCOCYANINFROM SPIRULINALAXA IN E. COLI BL21(DE3)

Anbar Science Journal
This study focuses on the cloning and expression of the β-subunit gene of C-phycocyanin (C-PC) from Spirulina laxa in Escherichia coli BL21 (DE3). C-phycocyanin, a significant light-harvesting protein found in cyanobacteria, is known for its pharmacological properties, including antioxidant and antifungal activities.
Noor K. Shaker, Harith K. Buniya
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[Cloning and expression of caiDE genes in E. coli BL21(DE3)].

Wei sheng wu xue bao = Acta microbiologica Sinica, 2004
The caiDE genes for carnitine racemase and its relative factor were cloned into pUC18 from the chromosomal DNA of E. coli MC4100 by shot-gun approach and have been verified by sequence analysis. The target plasmid was named as pJX393 and subcloned to form a transcription plasmid pDSW2.
J, Jiang, S, Duan, H, Zhang
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Enhanced anticoagulant activity of hirudin-i analogue co-expressed with arylsulfotransferase in periplasm of E. coli BL21(DE3)

Journal of Biotechnology, 2020
Hirudin, a blood anticoagulant, is the most potent natural thrombin inhibitor of leech origin. Its application is limited because it is difficult to obtain abundant natural hirudin directly from the leech. Although some bioengineering methods can significantly increase the production of hirudin, the reduced efficacy of recombinant hirudin (rH) remains ...
Kamal, Rai, Xiaohui, Chu, Zixian, Bao, Yunlong, Liang, Xingang, Wang, Junqing, Yang, Mo, Xian, Yue, Sun, Rui, Nian   +8 more
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[Fusion expression and identification of angiostatin and endostatin in E. coli BL21(DE3)].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 2007
Angiostatin(AS) and endostatin(ES) are both potent endogenous angiogenesis inhibitors, and the combination of AS and ES has been shown to have synergistic antiangiogenic effects. Here we report the fusion protein AS-ES expressed in E. coli which has antiangiogenic effects.
Xiaoyan, Mao, Weibin, Liu, Shihong, Fu, Rende, Li, Guodong, Liang   +4 more
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Effect of Physical Parameters on Production of ᴅ-Xylonic Acid using Recombinant E. coli BL21 (DE3)

Materials Today: Proceedings, 2019
Abstract ᴅ-xylonic acid is a five-carbon organic acid that has gain increasing interest due to its broadly flexible application in fields such as foods, pharmaceutical and agriculture. Microbial production of ᴅ-xylonic acid can be done by using many species of Pseudomonas, Acetobacter, Gluconobacter and the latest finding was by engineered ...
N.A.M. Rodzri, W.S.W.M. Zain, R.M.A. Hanapiah, R.A. Samah, R.M. Illias   +4 more
openaire   +1 more source